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Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats
Cardiovascular (CV) disease is the main cause of death in nonalcoholic fatty liver disease (NAFLD), a clinical condition without any approved pharmacological therapy. Thus, we investigated the effects of ornithine aspartate (LOLA) and/or Vitamin E (VitE) on CV parameters in a steatohepatitis experim...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775092/ https://www.ncbi.nlm.nih.gov/pubmed/36551202 http://dx.doi.org/10.3390/biom12121773 |
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author | de Freitas, Laura Bainy Rodrigues Longo, Larisse Filippi-Chiela, Eduardo de Souza, Valessa Emanoele Gabriel Behrens, Luiza Pereira, Matheus Henrique Mariano Leonhard, Luiza Cecília Zanettini, Giulianna Pinzon, Carlos Eduardo Luchese, Eduardo Lima, Guilherme Jorge Semmelmann Pereira Cerski, Carlos Thadeu Uribe-Cruz, Carolina Álvares-da-Silva, Mário Reis |
author_facet | de Freitas, Laura Bainy Rodrigues Longo, Larisse Filippi-Chiela, Eduardo de Souza, Valessa Emanoele Gabriel Behrens, Luiza Pereira, Matheus Henrique Mariano Leonhard, Luiza Cecília Zanettini, Giulianna Pinzon, Carlos Eduardo Luchese, Eduardo Lima, Guilherme Jorge Semmelmann Pereira Cerski, Carlos Thadeu Uribe-Cruz, Carolina Álvares-da-Silva, Mário Reis |
author_sort | de Freitas, Laura Bainy Rodrigues |
collection | PubMed |
description | Cardiovascular (CV) disease is the main cause of death in nonalcoholic fatty liver disease (NAFLD), a clinical condition without any approved pharmacological therapy. Thus, we investigated the effects of ornithine aspartate (LOLA) and/or Vitamin E (VitE) on CV parameters in a steatohepatitis experimental model. Adult Sprague Dawley rats were randomly assigned (10 animals each) and treated from 16 to 28 weeks with gavage as follows: controls (standard diet plus distilled water (DW)), NAFLD (high-fat choline-deficient diet (HFCD) plus DW), NAFLD+LOLA (HFCD plus LOLA (200 mg/kg/day)), NAFLD+VitE (HFCD plus VitE (150 mg twice a week)) or NAFLD+LOLA+VitE in the same doses. Atherogenic ratios were higher in NAFLD when compared with NAFLD+LOLA+VitE and controls (p < 0.05). Serum concentration of IL-1β, IL-6, TNF-α, MCP-1, e-selectin, ICAM-1, and PAI-1 were not different in intervention groups and controls (p > 0.05). NAFLD+LOLA decreased miR-122, miR-33a, and miR-186 (p < 0.05, for all) in relation to NAFLD. NAFLD+LOLA+VitE decreased miR-122, miR-33a and miR-186, and increased miR-126 (p < 0.05, for all) in comparison to NAFLD and NAFLD+VitE. NAFLD+LOLA and NAFLD+LOLA+VitE prevented liver collagen deposition (p = 0.006) in comparison to NAFLD. Normal cardiac fibers (size and shape) were lower in NAFLD in relation to the others; and the inverse was reported for the percentage of regular hypertrophic cardiomyocytes. NAFLD+LOLA+VitE promoted a significant improvement in atherogenic dyslipidemia, liver fibrosis, and paracrine signaling of lipid metabolism and endothelial dysfunction. This association should be further explored in the treatment of NAFLD-associated CV risk factors. |
format | Online Article Text |
id | pubmed-9775092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97750922022-12-23 Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats de Freitas, Laura Bainy Rodrigues Longo, Larisse Filippi-Chiela, Eduardo de Souza, Valessa Emanoele Gabriel Behrens, Luiza Pereira, Matheus Henrique Mariano Leonhard, Luiza Cecília Zanettini, Giulianna Pinzon, Carlos Eduardo Luchese, Eduardo Lima, Guilherme Jorge Semmelmann Pereira Cerski, Carlos Thadeu Uribe-Cruz, Carolina Álvares-da-Silva, Mário Reis Biomolecules Article Cardiovascular (CV) disease is the main cause of death in nonalcoholic fatty liver disease (NAFLD), a clinical condition without any approved pharmacological therapy. Thus, we investigated the effects of ornithine aspartate (LOLA) and/or Vitamin E (VitE) on CV parameters in a steatohepatitis experimental model. Adult Sprague Dawley rats were randomly assigned (10 animals each) and treated from 16 to 28 weeks with gavage as follows: controls (standard diet plus distilled water (DW)), NAFLD (high-fat choline-deficient diet (HFCD) plus DW), NAFLD+LOLA (HFCD plus LOLA (200 mg/kg/day)), NAFLD+VitE (HFCD plus VitE (150 mg twice a week)) or NAFLD+LOLA+VitE in the same doses. Atherogenic ratios were higher in NAFLD when compared with NAFLD+LOLA+VitE and controls (p < 0.05). Serum concentration of IL-1β, IL-6, TNF-α, MCP-1, e-selectin, ICAM-1, and PAI-1 were not different in intervention groups and controls (p > 0.05). NAFLD+LOLA decreased miR-122, miR-33a, and miR-186 (p < 0.05, for all) in relation to NAFLD. NAFLD+LOLA+VitE decreased miR-122, miR-33a and miR-186, and increased miR-126 (p < 0.05, for all) in comparison to NAFLD and NAFLD+VitE. NAFLD+LOLA and NAFLD+LOLA+VitE prevented liver collagen deposition (p = 0.006) in comparison to NAFLD. Normal cardiac fibers (size and shape) were lower in NAFLD in relation to the others; and the inverse was reported for the percentage of regular hypertrophic cardiomyocytes. NAFLD+LOLA+VitE promoted a significant improvement in atherogenic dyslipidemia, liver fibrosis, and paracrine signaling of lipid metabolism and endothelial dysfunction. This association should be further explored in the treatment of NAFLD-associated CV risk factors. MDPI 2022-11-28 /pmc/articles/PMC9775092/ /pubmed/36551202 http://dx.doi.org/10.3390/biom12121773 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Freitas, Laura Bainy Rodrigues Longo, Larisse Filippi-Chiela, Eduardo de Souza, Valessa Emanoele Gabriel Behrens, Luiza Pereira, Matheus Henrique Mariano Leonhard, Luiza Cecília Zanettini, Giulianna Pinzon, Carlos Eduardo Luchese, Eduardo Lima, Guilherme Jorge Semmelmann Pereira Cerski, Carlos Thadeu Uribe-Cruz, Carolina Álvares-da-Silva, Mário Reis Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats |
title | Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats |
title_full | Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats |
title_fullStr | Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats |
title_full_unstemmed | Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats |
title_short | Ornithine Aspartate and Vitamin-E Combination Has Beneficial Effects on Cardiovascular Risk Factors in an Animal Model of Nonalcoholic Fatty Liver Disease in Rats |
title_sort | ornithine aspartate and vitamin-e combination has beneficial effects on cardiovascular risk factors in an animal model of nonalcoholic fatty liver disease in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775092/ https://www.ncbi.nlm.nih.gov/pubmed/36551202 http://dx.doi.org/10.3390/biom12121773 |
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