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Distal Mutations Shape Substrate-Binding Sites during Evolution of a Metallo-Oxidase into a Laccase

[Image: see text] Laccases are in increasing demand as innovative solutions in the biorefinery fields. Here, we combine mutagenesis with structural, kinetic, and in silico analyses to characterize the molecular features that cause the evolution of a hyperthermostable metallo-oxidase from the multico...

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Detalles Bibliográficos
Autores principales:	Brissos, Vânia, Borges, Patrícia T., Núñez-Franco, Reyes, Lucas, Maria Fátima, Frazão, Carlos, Monza, Emanuele, Masgrau, Laura, Cordeiro, Tiago N., Martins, Lígia O.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775220/ https://www.ncbi.nlm.nih.gov/pubmed/36567772 http://dx.doi.org/10.1021/acscatal.2c00336

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