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Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers

SIMPLE SUMMARY: The implementation of new strategies to define beneficial bacteria for improving tolerance to stressful conditions in plants is becoming a very relevant topic. The use of microbiota associated with seeds may generate a new solution to improving the efficiency of bioinoculants. Legume...

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Autores principales: Niza-Costa, Marla, Rodríguez-dos Santos, Ana Sofía, Rebelo-Romão, Inês, Ferrer, María Victoria, Sequero López, Cristina, Vílchez, Juan Ignacio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775229/
https://www.ncbi.nlm.nih.gov/pubmed/36552347
http://dx.doi.org/10.3390/biology11121838
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author Niza-Costa, Marla
Rodríguez-dos Santos, Ana Sofía
Rebelo-Romão, Inês
Ferrer, María Victoria
Sequero López, Cristina
Vílchez, Juan Ignacio
author_facet Niza-Costa, Marla
Rodríguez-dos Santos, Ana Sofía
Rebelo-Romão, Inês
Ferrer, María Victoria
Sequero López, Cristina
Vílchez, Juan Ignacio
author_sort Niza-Costa, Marla
collection PubMed
description SIMPLE SUMMARY: The implementation of new strategies to define beneficial bacteria for improving tolerance to stressful conditions in plants is becoming a very relevant topic. The use of microbiota associated with seeds may generate a new solution to improving the efficiency of bioinoculants. Legume production suffers from increasingly accentuated climatic conditions such as heat and drought periods. This group of plants can be especially protected against stress by applying strains isolated from seeds of wild legume varieties. This study shows how strains isolated from seeds of alfalfa and pitch clover are capable of improving the development of lentil plants under drought conditions. ABSTRACT: Agricultural production is being affected by increasingly harsh conditions caused by climate change. The vast majority of crops suffer growth and yield declines due to a lack of water or intense heat. Hence, commercial legume crops suffer intense losses of production (20–80%). This situation is even more noticeable in plants used as fodder for animals, such as alfalfa and pitch trefoil, since their productivity is linked not only to the number of seeds produced, but also to the vegetative growth of the plant itself. Thus, we decided to study the microbiota associated with their seeds in different locations on the Iberian Peninsula, with the aim of identifying culturable bacteria strains that have adapted to harsh environments and that can be used as biotreatments to improve plant growth and resistance to stress. As potentially inherited microbiota, they may also represent a treatment with medium- and long-term adaptative effects. Hence, isolated strains showed no clear relationship with their geographical sampling location, but had about 50% internal similarity with their model plants. Moreover, out of the 51 strains isolated, about 80% were capable of producing biofilms; around 50% produced mid/high concentrations of auxins and grew notably in ACC medium; only 15% were characterized as xerotolerant, while more than 75% were able to sporulate; and finally, 65% produced siderophores and more than 40% produced compounds to solubilize phosphates. Thus, Paenibacillus amylolyticus BB B2-A, Paenibacillus xylanexedens MS M1-C, Paenibacillus pabuli BB Oeiras A, Stenotrophomonas maltophilia MS M1-B and Enterobacter hormaechei BB B2-C strains were tested as plant bioinoculants in lentil plants (Lens culinaris Medik.), showing promising results as future treatments to improve plant growth under stressful conditions.
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spelling pubmed-97752292022-12-23 Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers Niza-Costa, Marla Rodríguez-dos Santos, Ana Sofía Rebelo-Romão, Inês Ferrer, María Victoria Sequero López, Cristina Vílchez, Juan Ignacio Biology (Basel) Article SIMPLE SUMMARY: The implementation of new strategies to define beneficial bacteria for improving tolerance to stressful conditions in plants is becoming a very relevant topic. The use of microbiota associated with seeds may generate a new solution to improving the efficiency of bioinoculants. Legume production suffers from increasingly accentuated climatic conditions such as heat and drought periods. This group of plants can be especially protected against stress by applying strains isolated from seeds of wild legume varieties. This study shows how strains isolated from seeds of alfalfa and pitch clover are capable of improving the development of lentil plants under drought conditions. ABSTRACT: Agricultural production is being affected by increasingly harsh conditions caused by climate change. The vast majority of crops suffer growth and yield declines due to a lack of water or intense heat. Hence, commercial legume crops suffer intense losses of production (20–80%). This situation is even more noticeable in plants used as fodder for animals, such as alfalfa and pitch trefoil, since their productivity is linked not only to the number of seeds produced, but also to the vegetative growth of the plant itself. Thus, we decided to study the microbiota associated with their seeds in different locations on the Iberian Peninsula, with the aim of identifying culturable bacteria strains that have adapted to harsh environments and that can be used as biotreatments to improve plant growth and resistance to stress. As potentially inherited microbiota, they may also represent a treatment with medium- and long-term adaptative effects. Hence, isolated strains showed no clear relationship with their geographical sampling location, but had about 50% internal similarity with their model plants. Moreover, out of the 51 strains isolated, about 80% were capable of producing biofilms; around 50% produced mid/high concentrations of auxins and grew notably in ACC medium; only 15% were characterized as xerotolerant, while more than 75% were able to sporulate; and finally, 65% produced siderophores and more than 40% produced compounds to solubilize phosphates. Thus, Paenibacillus amylolyticus BB B2-A, Paenibacillus xylanexedens MS M1-C, Paenibacillus pabuli BB Oeiras A, Stenotrophomonas maltophilia MS M1-B and Enterobacter hormaechei BB B2-C strains were tested as plant bioinoculants in lentil plants (Lens culinaris Medik.), showing promising results as future treatments to improve plant growth under stressful conditions. MDPI 2022-12-16 /pmc/articles/PMC9775229/ /pubmed/36552347 http://dx.doi.org/10.3390/biology11121838 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Niza-Costa, Marla
Rodríguez-dos Santos, Ana Sofía
Rebelo-Romão, Inês
Ferrer, María Victoria
Sequero López, Cristina
Vílchez, Juan Ignacio
Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers
title Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers
title_full Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers
title_fullStr Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers
title_full_unstemmed Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers
title_short Geographically Disperse, Culturable Seed-Associated Microbiota in Forage Plants of Alfalfa (Medicago sativa L.) and Pitch Clover (Bituminaria bituminosa L.): Characterization of Beneficial Inherited Strains as Plant Stress-Tolerance Enhancers
title_sort geographically disperse, culturable seed-associated microbiota in forage plants of alfalfa (medicago sativa l.) and pitch clover (bituminaria bituminosa l.): characterization of beneficial inherited strains as plant stress-tolerance enhancers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775229/
https://www.ncbi.nlm.nih.gov/pubmed/36552347
http://dx.doi.org/10.3390/biology11121838
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