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Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection

Immunodiagnostics have been widely used in the detection of disease biomarkers. The conventional immunological tests in central laboratories require expensive equipment and, for non-specialists, the tests are technically demanding and time-consuming, which has prevented their use by the public. Thus...

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Detalles Bibliográficos
Autores principales: Lian, Cheng, Young, Dan, Randall, Richard E., Samuel, Ifor D. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775261/
https://www.ncbi.nlm.nih.gov/pubmed/36551092
http://dx.doi.org/10.3390/bios12121125
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author Lian, Cheng
Young, Dan
Randall, Richard E.
Samuel, Ifor D. W.
author_facet Lian, Cheng
Young, Dan
Randall, Richard E.
Samuel, Ifor D. W.
author_sort Lian, Cheng
collection PubMed
description Immunodiagnostics have been widely used in the detection of disease biomarkers. The conventional immunological tests in central laboratories require expensive equipment and, for non-specialists, the tests are technically demanding and time-consuming, which has prevented their use by the public. Thus, point-of-care tests (POCT), such as lateral flow immunoassays, are being, or have been, developed as more convenient and low-cost methods for immunodiagnostics. However, the sensitivity of such tests is often a concern. Here, a fluorescence-linked immunosorbent assay (FLISA) using organic light-emitting diodes (OLEDs) as excitation light sources was investigated as a way forward for the development of compact and sensitive POCTs. Phycoerythrin (PE) was selected as the fluorescent dye, and OLEDs were designed with different emission spectra. The leakage light of different OLEDs for exciting PE was then investigated to reduce the background noise and improve the sensitivity of the system. Finally, as proof-of-principle that OLED-based technology can be successfully further developed for POCT, antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human serum was detected by OLED−FLISA.
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spelling pubmed-97752612022-12-23 Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection Lian, Cheng Young, Dan Randall, Richard E. Samuel, Ifor D. W. Biosensors (Basel) Article Immunodiagnostics have been widely used in the detection of disease biomarkers. The conventional immunological tests in central laboratories require expensive equipment and, for non-specialists, the tests are technically demanding and time-consuming, which has prevented their use by the public. Thus, point-of-care tests (POCT), such as lateral flow immunoassays, are being, or have been, developed as more convenient and low-cost methods for immunodiagnostics. However, the sensitivity of such tests is often a concern. Here, a fluorescence-linked immunosorbent assay (FLISA) using organic light-emitting diodes (OLEDs) as excitation light sources was investigated as a way forward for the development of compact and sensitive POCTs. Phycoerythrin (PE) was selected as the fluorescent dye, and OLEDs were designed with different emission spectra. The leakage light of different OLEDs for exciting PE was then investigated to reduce the background noise and improve the sensitivity of the system. Finally, as proof-of-principle that OLED-based technology can be successfully further developed for POCT, antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human serum was detected by OLED−FLISA. MDPI 2022-12-05 /pmc/articles/PMC9775261/ /pubmed/36551092 http://dx.doi.org/10.3390/bios12121125 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lian, Cheng
Young, Dan
Randall, Richard E.
Samuel, Ifor D. W.
Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
title Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
title_full Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
title_fullStr Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
title_full_unstemmed Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
title_short Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
title_sort organic light-emitting diode based fluorescence-linked immunosorbent assay for sars-cov-2 antibody detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775261/
https://www.ncbi.nlm.nih.gov/pubmed/36551092
http://dx.doi.org/10.3390/bios12121125
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