Cargando…
Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection
Immunodiagnostics have been widely used in the detection of disease biomarkers. The conventional immunological tests in central laboratories require expensive equipment and, for non-specialists, the tests are technically demanding and time-consuming, which has prevented their use by the public. Thus...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775261/ https://www.ncbi.nlm.nih.gov/pubmed/36551092 http://dx.doi.org/10.3390/bios12121125 |
_version_ | 1784855600673325056 |
---|---|
author | Lian, Cheng Young, Dan Randall, Richard E. Samuel, Ifor D. W. |
author_facet | Lian, Cheng Young, Dan Randall, Richard E. Samuel, Ifor D. W. |
author_sort | Lian, Cheng |
collection | PubMed |
description | Immunodiagnostics have been widely used in the detection of disease biomarkers. The conventional immunological tests in central laboratories require expensive equipment and, for non-specialists, the tests are technically demanding and time-consuming, which has prevented their use by the public. Thus, point-of-care tests (POCT), such as lateral flow immunoassays, are being, or have been, developed as more convenient and low-cost methods for immunodiagnostics. However, the sensitivity of such tests is often a concern. Here, a fluorescence-linked immunosorbent assay (FLISA) using organic light-emitting diodes (OLEDs) as excitation light sources was investigated as a way forward for the development of compact and sensitive POCTs. Phycoerythrin (PE) was selected as the fluorescent dye, and OLEDs were designed with different emission spectra. The leakage light of different OLEDs for exciting PE was then investigated to reduce the background noise and improve the sensitivity of the system. Finally, as proof-of-principle that OLED-based technology can be successfully further developed for POCT, antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human serum was detected by OLED−FLISA. |
format | Online Article Text |
id | pubmed-9775261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97752612022-12-23 Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection Lian, Cheng Young, Dan Randall, Richard E. Samuel, Ifor D. W. Biosensors (Basel) Article Immunodiagnostics have been widely used in the detection of disease biomarkers. The conventional immunological tests in central laboratories require expensive equipment and, for non-specialists, the tests are technically demanding and time-consuming, which has prevented their use by the public. Thus, point-of-care tests (POCT), such as lateral flow immunoassays, are being, or have been, developed as more convenient and low-cost methods for immunodiagnostics. However, the sensitivity of such tests is often a concern. Here, a fluorescence-linked immunosorbent assay (FLISA) using organic light-emitting diodes (OLEDs) as excitation light sources was investigated as a way forward for the development of compact and sensitive POCTs. Phycoerythrin (PE) was selected as the fluorescent dye, and OLEDs were designed with different emission spectra. The leakage light of different OLEDs for exciting PE was then investigated to reduce the background noise and improve the sensitivity of the system. Finally, as proof-of-principle that OLED-based technology can be successfully further developed for POCT, antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human serum was detected by OLED−FLISA. MDPI 2022-12-05 /pmc/articles/PMC9775261/ /pubmed/36551092 http://dx.doi.org/10.3390/bios12121125 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lian, Cheng Young, Dan Randall, Richard E. Samuel, Ifor D. W. Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection |
title | Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection |
title_full | Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection |
title_fullStr | Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection |
title_full_unstemmed | Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection |
title_short | Organic Light-Emitting Diode Based Fluorescence-Linked Immunosorbent Assay for SARS-CoV-2 Antibody Detection |
title_sort | organic light-emitting diode based fluorescence-linked immunosorbent assay for sars-cov-2 antibody detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775261/ https://www.ncbi.nlm.nih.gov/pubmed/36551092 http://dx.doi.org/10.3390/bios12121125 |
work_keys_str_mv | AT liancheng organiclightemittingdiodebasedfluorescencelinkedimmunosorbentassayforsarscov2antibodydetection AT youngdan organiclightemittingdiodebasedfluorescencelinkedimmunosorbentassayforsarscov2antibodydetection AT randallricharde organiclightemittingdiodebasedfluorescencelinkedimmunosorbentassayforsarscov2antibodydetection AT samuelifordw organiclightemittingdiodebasedfluorescencelinkedimmunosorbentassayforsarscov2antibodydetection |