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Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously eval...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775334/ https://www.ncbi.nlm.nih.gov/pubmed/36551268 http://dx.doi.org/10.3390/biom12121838 |
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author | Vasilakopoulou, Paraskevi B. Gousgouni, Aimilia-Tatiana Yanni, Amalia E. Kostomitsopoulos, Nikolaos Karathanos, Vaios T. Chiou, Antonia |
author_facet | Vasilakopoulou, Paraskevi B. Gousgouni, Aimilia-Tatiana Yanni, Amalia E. Kostomitsopoulos, Nikolaos Karathanos, Vaios T. Chiou, Antonia |
author_sort | Vasilakopoulou, Paraskevi B. |
collection | PubMed |
description | Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food. |
format | Online Article Text |
id | pubmed-9775334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97753342022-12-23 Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats Vasilakopoulou, Paraskevi B. Gousgouni, Aimilia-Tatiana Yanni, Amalia E. Kostomitsopoulos, Nikolaos Karathanos, Vaios T. Chiou, Antonia Biomolecules Article Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food. MDPI 2022-12-08 /pmc/articles/PMC9775334/ /pubmed/36551268 http://dx.doi.org/10.3390/biom12121838 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vasilakopoulou, Paraskevi B. Gousgouni, Aimilia-Tatiana Yanni, Amalia E. Kostomitsopoulos, Nikolaos Karathanos, Vaios T. Chiou, Antonia Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats |
title | Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats |
title_full | Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats |
title_fullStr | Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats |
title_full_unstemmed | Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats |
title_short | Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats |
title_sort | polar phenol detection in plasma and serum: insights on sample pre-treatment for lc/ms analysis and application on the serum of corinthian currant-fed rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775334/ https://www.ncbi.nlm.nih.gov/pubmed/36551268 http://dx.doi.org/10.3390/biom12121838 |
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