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Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously eval...

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Autores principales: Vasilakopoulou, Paraskevi B., Gousgouni, Aimilia-Tatiana, Yanni, Amalia E., Kostomitsopoulos, Nikolaos, Karathanos, Vaios T., Chiou, Antonia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775334/
https://www.ncbi.nlm.nih.gov/pubmed/36551268
http://dx.doi.org/10.3390/biom12121838
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author Vasilakopoulou, Paraskevi B.
Gousgouni, Aimilia-Tatiana
Yanni, Amalia E.
Kostomitsopoulos, Nikolaos
Karathanos, Vaios T.
Chiou, Antonia
author_facet Vasilakopoulou, Paraskevi B.
Gousgouni, Aimilia-Tatiana
Yanni, Amalia E.
Kostomitsopoulos, Nikolaos
Karathanos, Vaios T.
Chiou, Antonia
author_sort Vasilakopoulou, Paraskevi B.
collection PubMed
description Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food.
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spelling pubmed-97753342022-12-23 Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats Vasilakopoulou, Paraskevi B. Gousgouni, Aimilia-Tatiana Yanni, Amalia E. Kostomitsopoulos, Nikolaos Karathanos, Vaios T. Chiou, Antonia Biomolecules Article Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food. MDPI 2022-12-08 /pmc/articles/PMC9775334/ /pubmed/36551268 http://dx.doi.org/10.3390/biom12121838 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vasilakopoulou, Paraskevi B.
Gousgouni, Aimilia-Tatiana
Yanni, Amalia E.
Kostomitsopoulos, Nikolaos
Karathanos, Vaios T.
Chiou, Antonia
Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
title Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
title_full Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
title_fullStr Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
title_full_unstemmed Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
title_short Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats
title_sort polar phenol detection in plasma and serum: insights on sample pre-treatment for lc/ms analysis and application on the serum of corinthian currant-fed rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775334/
https://www.ncbi.nlm.nih.gov/pubmed/36551268
http://dx.doi.org/10.3390/biom12121838
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