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Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha

Antioxidants are responsible for many beneficial health effects and are highly present in natural products, such as kombucha. Biosensors’ development targeting antioxidants and phytomarkers are an active research field. This work aimed to propose a voltammetric polyphenolxidase (Cordia superba) bios...

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Autores principales: Batista, Erica A., Pereira, Marx O. A., Macêdo, Isaac Y. L., Machado, Fabio B., Moreno, Emily K. G., Diniz, Elgia P., Frazzão, Italo G. V., Bernardes, Lorrayne S. C., Oliveira, Severino C. B., Gil, Eric S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775402/
https://www.ncbi.nlm.nih.gov/pubmed/36551079
http://dx.doi.org/10.3390/bios12121112
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author Batista, Erica A.
Pereira, Marx O. A.
Macêdo, Isaac Y. L.
Machado, Fabio B.
Moreno, Emily K. G.
Diniz, Elgia P.
Frazzão, Italo G. V.
Bernardes, Lorrayne S. C.
Oliveira, Severino C. B.
Gil, Eric S.
author_facet Batista, Erica A.
Pereira, Marx O. A.
Macêdo, Isaac Y. L.
Machado, Fabio B.
Moreno, Emily K. G.
Diniz, Elgia P.
Frazzão, Italo G. V.
Bernardes, Lorrayne S. C.
Oliveira, Severino C. B.
Gil, Eric S.
author_sort Batista, Erica A.
collection PubMed
description Antioxidants are responsible for many beneficial health effects and are highly present in natural products, such as kombucha. Biosensors’ development targeting antioxidants and phytomarkers are an active research field. This work aimed to propose a voltammetric polyphenolxidase (Cordia superba) biosensor for catechin and total phenolic compounds quantification in kombucha samples. Optimizations were performed on the biosensor of Cordia superba to improve the accuracy and selectivity, such as enzyme–substrate interaction time, analytical responses for different patterns and signal differences with the carbon paste and modified carbon paste electrode. Kombucha probiotic drink samples were fermented for 7 to 14 days at a controlled temperature (28 ± 2 °C). A linear curve was made for catechin with a range of 10.00 to 60.00 µM, with a limit of detection of 0.13 µM and limit of quantification of 0.39 µM. The biosensor proposed in this work was efficient in determining the patterns of phenolic compounds in kombucha.
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spelling pubmed-97754022022-12-23 Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha Batista, Erica A. Pereira, Marx O. A. Macêdo, Isaac Y. L. Machado, Fabio B. Moreno, Emily K. G. Diniz, Elgia P. Frazzão, Italo G. V. Bernardes, Lorrayne S. C. Oliveira, Severino C. B. Gil, Eric S. Biosensors (Basel) Communication Antioxidants are responsible for many beneficial health effects and are highly present in natural products, such as kombucha. Biosensors’ development targeting antioxidants and phytomarkers are an active research field. This work aimed to propose a voltammetric polyphenolxidase (Cordia superba) biosensor for catechin and total phenolic compounds quantification in kombucha samples. Optimizations were performed on the biosensor of Cordia superba to improve the accuracy and selectivity, such as enzyme–substrate interaction time, analytical responses for different patterns and signal differences with the carbon paste and modified carbon paste electrode. Kombucha probiotic drink samples were fermented for 7 to 14 days at a controlled temperature (28 ± 2 °C). A linear curve was made for catechin with a range of 10.00 to 60.00 µM, with a limit of detection of 0.13 µM and limit of quantification of 0.39 µM. The biosensor proposed in this work was efficient in determining the patterns of phenolic compounds in kombucha. MDPI 2022-12-01 /pmc/articles/PMC9775402/ /pubmed/36551079 http://dx.doi.org/10.3390/bios12121112 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Batista, Erica A.
Pereira, Marx O. A.
Macêdo, Isaac Y. L.
Machado, Fabio B.
Moreno, Emily K. G.
Diniz, Elgia P.
Frazzão, Italo G. V.
Bernardes, Lorrayne S. C.
Oliveira, Severino C. B.
Gil, Eric S.
Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha
title Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha
title_full Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha
title_fullStr Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha
title_full_unstemmed Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha
title_short Electroanalytical Enzyme Biosensor Based on Cordia superba Enzyme Extract for the Detection of Phytomarkers in Kombucha
title_sort electroanalytical enzyme biosensor based on cordia superba enzyme extract for the detection of phytomarkers in kombucha
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775402/
https://www.ncbi.nlm.nih.gov/pubmed/36551079
http://dx.doi.org/10.3390/bios12121112
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