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Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma
Background: Oral squamous cell carcinoma (OSCC) is one of the most commonly detected neoplasms worldwide. Not all mechanisms associated with cell cycle disturbances are known in OSCC. Examples of genes involved in the control of the cell cycle are CDKN2A, MDM2, E2F2 and LTF. The aim of this study wa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775533/ https://www.ncbi.nlm.nih.gov/pubmed/36551770 http://dx.doi.org/10.3390/biomedicines10123011 |
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author | Gołąbek, Karolina Rączka, Grzegorz Gaździcka, Jadwiga Miśkiewicz-Orczyk, Katarzyna Zięba, Natalia Krakowczyk, Łukasz Misiołek, Maciej Strzelczyk, Joanna Katarzyna |
author_facet | Gołąbek, Karolina Rączka, Grzegorz Gaździcka, Jadwiga Miśkiewicz-Orczyk, Katarzyna Zięba, Natalia Krakowczyk, Łukasz Misiołek, Maciej Strzelczyk, Joanna Katarzyna |
author_sort | Gołąbek, Karolina |
collection | PubMed |
description | Background: Oral squamous cell carcinoma (OSCC) is one of the most commonly detected neoplasms worldwide. Not all mechanisms associated with cell cycle disturbances are known in OSCC. Examples of genes involved in the control of the cell cycle are CDKN2A, MDM2, E2F2 and LTF. The aim of this study was to examine the possible association between CDKN2A, MDM2, E2F2 and LTF mRNA expression and influence on clinical variables. Methods: The study group consisted of 88 Polish patients. The gene expression levels were assessed by quantitative reverse transcription PCR. Results: We found no statistically significant differences in the expression level of CDKN2A, MDM2, E2F2 and LTF genes in tumour samples compared to margin samples. No association was found between the gene expression levels and clinical parameters, except E2F2. The patients with G2 tumours had a significantly higher gene expression level of E2F2 than patients with low-grade G1 tumours. Conclusions: We have not demonstrated that a change in expression profiles of genes has a significant impact on the pathogenesis of OSCC. It may also be useful to conduct further studies on the use of E2F2 expression profile changes as a factor to describe the invasiveness and dynamics of OSCC development. |
format | Online Article Text |
id | pubmed-9775533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97755332022-12-23 Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma Gołąbek, Karolina Rączka, Grzegorz Gaździcka, Jadwiga Miśkiewicz-Orczyk, Katarzyna Zięba, Natalia Krakowczyk, Łukasz Misiołek, Maciej Strzelczyk, Joanna Katarzyna Biomedicines Article Background: Oral squamous cell carcinoma (OSCC) is one of the most commonly detected neoplasms worldwide. Not all mechanisms associated with cell cycle disturbances are known in OSCC. Examples of genes involved in the control of the cell cycle are CDKN2A, MDM2, E2F2 and LTF. The aim of this study was to examine the possible association between CDKN2A, MDM2, E2F2 and LTF mRNA expression and influence on clinical variables. Methods: The study group consisted of 88 Polish patients. The gene expression levels were assessed by quantitative reverse transcription PCR. Results: We found no statistically significant differences in the expression level of CDKN2A, MDM2, E2F2 and LTF genes in tumour samples compared to margin samples. No association was found between the gene expression levels and clinical parameters, except E2F2. The patients with G2 tumours had a significantly higher gene expression level of E2F2 than patients with low-grade G1 tumours. Conclusions: We have not demonstrated that a change in expression profiles of genes has a significant impact on the pathogenesis of OSCC. It may also be useful to conduct further studies on the use of E2F2 expression profile changes as a factor to describe the invasiveness and dynamics of OSCC development. MDPI 2022-11-23 /pmc/articles/PMC9775533/ /pubmed/36551770 http://dx.doi.org/10.3390/biomedicines10123011 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gołąbek, Karolina Rączka, Grzegorz Gaździcka, Jadwiga Miśkiewicz-Orczyk, Katarzyna Zięba, Natalia Krakowczyk, Łukasz Misiołek, Maciej Strzelczyk, Joanna Katarzyna Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma |
title | Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma |
title_full | Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma |
title_fullStr | Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma |
title_full_unstemmed | Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma |
title_short | Expression Profiles of CDKN2A, MDM2, E2F2 and LTF Genes in Oral Squamous Cell Carcinoma |
title_sort | expression profiles of cdkn2a, mdm2, e2f2 and ltf genes in oral squamous cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775533/ https://www.ncbi.nlm.nih.gov/pubmed/36551770 http://dx.doi.org/10.3390/biomedicines10123011 |
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