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A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair

Phytochemicals extracted from plant sources have potential remedial effects to cure a broad range of acute to severe illnesses and ailments. Quercetin is a flavonoid isolated from different dietary sources such as vegetables and fruits, exhibiting strong anti-inflammatory, anti-oxidative and non-tox...

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Autores principales: Dhasmana, Archna, Malik, Sumira, Ranjan, Anuj, Chauhan, Abhishek, Tashkandi, Hanaa M., Haque, Shafiul, Al-Raddadi, Rajaa, Harakeh, Steve, Zengin, Gökhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775630/
https://www.ncbi.nlm.nih.gov/pubmed/36551913
http://dx.doi.org/10.3390/biomedicines10123157
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author Dhasmana, Archna
Malik, Sumira
Ranjan, Anuj
Chauhan, Abhishek
Tashkandi, Hanaa M.
Haque, Shafiul
Al-Raddadi, Rajaa
Harakeh, Steve
Zengin, Gökhan
author_facet Dhasmana, Archna
Malik, Sumira
Ranjan, Anuj
Chauhan, Abhishek
Tashkandi, Hanaa M.
Haque, Shafiul
Al-Raddadi, Rajaa
Harakeh, Steve
Zengin, Gökhan
author_sort Dhasmana, Archna
collection PubMed
description Phytochemicals extracted from plant sources have potential remedial effects to cure a broad range of acute to severe illnesses and ailments. Quercetin is a flavonoid isolated from different dietary sources such as vegetables and fruits, exhibiting strong anti-inflammatory, anti-oxidative and non-toxic effects on the biological system. However, the direct uptake or administration of quercetin results in loss of functionality, poor activity, and reduced shelf-life of the bioactive component. In this regard, to improve the uptake, potential, and efficiency of natural components with prolonged storage in the host’s body after administration, numerous polymer drug delivery systems have been created. In the current study, three-dimensional (3D) porous (porosity: 92%; pore size: 81 µm) bio-polymeric foaming gelatin–alginate (GA) beads were fabricated for the entrapment of quercetin as therapeutic drug molecules—gelatin–alginate–quercetin (GAQ). The GAQ beads showed a significant uptake of quercetin molecules resulting in a reduction of reduced porosity up to 64% and pore size 63 µm with a controlled release profile in the PBS medium, showing ~80% release within 24 h. Subsequently, the GAQ beads showed remarkable antioxidant effects, and 95% anti-inflammatory activities along with remarkable in vitro cell culture growth and the observed proliferation of seeded fibroblast cells. Thus, we can conclude that the consistent release of quercetin showed non-toxic effects on normal cell lines and the bioactive surface of the GAQ beads enhances cell adhesion, proliferation, and differentiation more effectively than control GA polymeric beads and tissue culture plates (TCP). In summary, these findings show that these GAQ beads act as a biocompatible 3D construct with enormous potential in medicinal administration and tissue regeneration for accelerated healing.
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spelling pubmed-97756302022-12-23 A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair Dhasmana, Archna Malik, Sumira Ranjan, Anuj Chauhan, Abhishek Tashkandi, Hanaa M. Haque, Shafiul Al-Raddadi, Rajaa Harakeh, Steve Zengin, Gökhan Biomedicines Article Phytochemicals extracted from plant sources have potential remedial effects to cure a broad range of acute to severe illnesses and ailments. Quercetin is a flavonoid isolated from different dietary sources such as vegetables and fruits, exhibiting strong anti-inflammatory, anti-oxidative and non-toxic effects on the biological system. However, the direct uptake or administration of quercetin results in loss of functionality, poor activity, and reduced shelf-life of the bioactive component. In this regard, to improve the uptake, potential, and efficiency of natural components with prolonged storage in the host’s body after administration, numerous polymer drug delivery systems have been created. In the current study, three-dimensional (3D) porous (porosity: 92%; pore size: 81 µm) bio-polymeric foaming gelatin–alginate (GA) beads were fabricated for the entrapment of quercetin as therapeutic drug molecules—gelatin–alginate–quercetin (GAQ). The GAQ beads showed a significant uptake of quercetin molecules resulting in a reduction of reduced porosity up to 64% and pore size 63 µm with a controlled release profile in the PBS medium, showing ~80% release within 24 h. Subsequently, the GAQ beads showed remarkable antioxidant effects, and 95% anti-inflammatory activities along with remarkable in vitro cell culture growth and the observed proliferation of seeded fibroblast cells. Thus, we can conclude that the consistent release of quercetin showed non-toxic effects on normal cell lines and the bioactive surface of the GAQ beads enhances cell adhesion, proliferation, and differentiation more effectively than control GA polymeric beads and tissue culture plates (TCP). In summary, these findings show that these GAQ beads act as a biocompatible 3D construct with enormous potential in medicinal administration and tissue regeneration for accelerated healing. MDPI 2022-12-07 /pmc/articles/PMC9775630/ /pubmed/36551913 http://dx.doi.org/10.3390/biomedicines10123157 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dhasmana, Archna
Malik, Sumira
Ranjan, Anuj
Chauhan, Abhishek
Tashkandi, Hanaa M.
Haque, Shafiul
Al-Raddadi, Rajaa
Harakeh, Steve
Zengin, Gökhan
A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
title A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
title_full A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
title_fullStr A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
title_full_unstemmed A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
title_short A Bioengineered Quercetin-Loaded 3D Bio-Polymeric Graft for Tissue Regeneration and Repair
title_sort bioengineered quercetin-loaded 3d bio-polymeric graft for tissue regeneration and repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775630/
https://www.ncbi.nlm.nih.gov/pubmed/36551913
http://dx.doi.org/10.3390/biomedicines10123157
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