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Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam

Phase imaging of biochemical samples has been demonstrated for the first time at the Infrared Microspectroscopy (IRM) beamline of the Australian Synchrotron using the usually discarded near-IR (NIR) region of the synchrotron-IR beam. The synchrotron-IR beam at the Australian Synchrotron IRM beamline...

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Autores principales: Han, Molong, Smith, Daniel, Ng, Soon Hock, Katkus, Tomas, John Francis Rajeswary, Aravind Simon, Praveen, Periyasamy Angamuthu, Bambery, Keith R., Tobin, Mark J., Vongsvivut, Jitraporn, Juodkazis, Saulius, Anand, Vijayakumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775640/
https://www.ncbi.nlm.nih.gov/pubmed/36551040
http://dx.doi.org/10.3390/bios12121073
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author Han, Molong
Smith, Daniel
Ng, Soon Hock
Katkus, Tomas
John Francis Rajeswary, Aravind Simon
Praveen, Periyasamy Angamuthu
Bambery, Keith R.
Tobin, Mark J.
Vongsvivut, Jitraporn
Juodkazis, Saulius
Anand, Vijayakumar
author_facet Han, Molong
Smith, Daniel
Ng, Soon Hock
Katkus, Tomas
John Francis Rajeswary, Aravind Simon
Praveen, Periyasamy Angamuthu
Bambery, Keith R.
Tobin, Mark J.
Vongsvivut, Jitraporn
Juodkazis, Saulius
Anand, Vijayakumar
author_sort Han, Molong
collection PubMed
description Phase imaging of biochemical samples has been demonstrated for the first time at the Infrared Microspectroscopy (IRM) beamline of the Australian Synchrotron using the usually discarded near-IR (NIR) region of the synchrotron-IR beam. The synchrotron-IR beam at the Australian Synchrotron IRM beamline has a unique fork shaped intensity distribution as a result of the gold coated extraction mirror shape, which includes a central slit for rejection of the intense X-ray beam. The resulting beam configuration makes any imaging task challenging. For intensity imaging, the fork shaped beam is usually tightly focused to a point on the sample plane followed by a pixel-by-pixel scanning approach to record the image. In this study, a pinhole was aligned with one of the lobes of the fork shaped beam and the Airy diffraction pattern was used to illuminate biochemical samples. The diffracted light from the samples was captured using a NIR sensitive lensless camera. A rapid phase-retrieval algorithm was applied to the recorded intensity distributions to reconstruct the phase information. The preliminary results are promising to develop multimodal imaging capabilities at the IRM beamline of the Australian Synchrotron.
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spelling pubmed-97756402022-12-23 Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam Han, Molong Smith, Daniel Ng, Soon Hock Katkus, Tomas John Francis Rajeswary, Aravind Simon Praveen, Periyasamy Angamuthu Bambery, Keith R. Tobin, Mark J. Vongsvivut, Jitraporn Juodkazis, Saulius Anand, Vijayakumar Biosensors (Basel) Communication Phase imaging of biochemical samples has been demonstrated for the first time at the Infrared Microspectroscopy (IRM) beamline of the Australian Synchrotron using the usually discarded near-IR (NIR) region of the synchrotron-IR beam. The synchrotron-IR beam at the Australian Synchrotron IRM beamline has a unique fork shaped intensity distribution as a result of the gold coated extraction mirror shape, which includes a central slit for rejection of the intense X-ray beam. The resulting beam configuration makes any imaging task challenging. For intensity imaging, the fork shaped beam is usually tightly focused to a point on the sample plane followed by a pixel-by-pixel scanning approach to record the image. In this study, a pinhole was aligned with one of the lobes of the fork shaped beam and the Airy diffraction pattern was used to illuminate biochemical samples. The diffracted light from the samples was captured using a NIR sensitive lensless camera. A rapid phase-retrieval algorithm was applied to the recorded intensity distributions to reconstruct the phase information. The preliminary results are promising to develop multimodal imaging capabilities at the IRM beamline of the Australian Synchrotron. MDPI 2022-11-24 /pmc/articles/PMC9775640/ /pubmed/36551040 http://dx.doi.org/10.3390/bios12121073 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Han, Molong
Smith, Daniel
Ng, Soon Hock
Katkus, Tomas
John Francis Rajeswary, Aravind Simon
Praveen, Periyasamy Angamuthu
Bambery, Keith R.
Tobin, Mark J.
Vongsvivut, Jitraporn
Juodkazis, Saulius
Anand, Vijayakumar
Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
title Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
title_full Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
title_fullStr Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
title_full_unstemmed Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
title_short Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam
title_sort single shot lensless interferenceless phase imaging of biochemical samples using synchrotron near infrared beam
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9775640/
https://www.ncbi.nlm.nih.gov/pubmed/36551040
http://dx.doi.org/10.3390/bios12121073
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