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Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation

(1) Calculated panel-reactive antibody (CPRA) is a measure of sensitization based on unacceptable antigens (UAs). Determination of UAs based on single-antigen bead assays at allele or antigen levels may be inappropriate. We aimed to introduce eplets for better assessment of sensitization; (2) 900 re...

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Autores principales: Wu, Wenrui, Zhang, Huanxi, Tan, Jinghong, Fu, Qian, Li, Jun, Wu, Chenglin, Huang, Huiting, Xu, Bowen, Ling, Liuting, Liu, Longshan, Su, Xiaojun, Wang, Changxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9776513/
https://www.ncbi.nlm.nih.gov/pubmed/36552990
http://dx.doi.org/10.3390/diagnostics12122983
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author Wu, Wenrui
Zhang, Huanxi
Tan, Jinghong
Fu, Qian
Li, Jun
Wu, Chenglin
Huang, Huiting
Xu, Bowen
Ling, Liuting
Liu, Longshan
Su, Xiaojun
Wang, Changxi
author_facet Wu, Wenrui
Zhang, Huanxi
Tan, Jinghong
Fu, Qian
Li, Jun
Wu, Chenglin
Huang, Huiting
Xu, Bowen
Ling, Liuting
Liu, Longshan
Su, Xiaojun
Wang, Changxi
author_sort Wu, Wenrui
collection PubMed
description (1) Calculated panel-reactive antibody (CPRA) is a measure of sensitization based on unacceptable antigens (UAs). Determination of UAs based on single-antigen bead assays at allele or antigen levels may be inappropriate. We aimed to introduce eplets for better assessment of sensitization; (2) 900 recipients and 1427 donors were enrolled for candidate or donor pools, respectively. Eplets were from the HLA Epitope Registry. UAs were determined by anti-HLA antibodies identified using LIFECODES Single Antigen (LSA) kits. CPRA values were calculated using a simplified method of donor filtering; (3) HLA antigens containing all eplets of an HLA antigen in LSA kits (LSA antigen) were defined as eplet-predicted (EP) antigens, the reactivity of which could be predicted by that LSA antigen. High reactivity concordance was found between LSA and EP antigens. More HLA antigens were covered by EP antigens in the population than LSA antigens. CPRA values at the EP level were higher than at the allele level and lower than at the antigen level. The EP antigens facilitated UA determination for non-LSA antigens and avoided acute rejection; (4) UA determination using EP antigens can lead to more accurate assessment of sensitization, enabling a high probability of compatible organs and a low risk of adverse outcomes.
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spelling pubmed-97765132022-12-23 Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation Wu, Wenrui Zhang, Huanxi Tan, Jinghong Fu, Qian Li, Jun Wu, Chenglin Huang, Huiting Xu, Bowen Ling, Liuting Liu, Longshan Su, Xiaojun Wang, Changxi Diagnostics (Basel) Article (1) Calculated panel-reactive antibody (CPRA) is a measure of sensitization based on unacceptable antigens (UAs). Determination of UAs based on single-antigen bead assays at allele or antigen levels may be inappropriate. We aimed to introduce eplets for better assessment of sensitization; (2) 900 recipients and 1427 donors were enrolled for candidate or donor pools, respectively. Eplets were from the HLA Epitope Registry. UAs were determined by anti-HLA antibodies identified using LIFECODES Single Antigen (LSA) kits. CPRA values were calculated using a simplified method of donor filtering; (3) HLA antigens containing all eplets of an HLA antigen in LSA kits (LSA antigen) were defined as eplet-predicted (EP) antigens, the reactivity of which could be predicted by that LSA antigen. High reactivity concordance was found between LSA and EP antigens. More HLA antigens were covered by EP antigens in the population than LSA antigens. CPRA values at the EP level were higher than at the allele level and lower than at the antigen level. The EP antigens facilitated UA determination for non-LSA antigens and avoided acute rejection; (4) UA determination using EP antigens can lead to more accurate assessment of sensitization, enabling a high probability of compatible organs and a low risk of adverse outcomes. MDPI 2022-11-28 /pmc/articles/PMC9776513/ /pubmed/36552990 http://dx.doi.org/10.3390/diagnostics12122983 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Wenrui
Zhang, Huanxi
Tan, Jinghong
Fu, Qian
Li, Jun
Wu, Chenglin
Huang, Huiting
Xu, Bowen
Ling, Liuting
Liu, Longshan
Su, Xiaojun
Wang, Changxi
Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation
title Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation
title_full Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation
title_fullStr Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation
title_full_unstemmed Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation
title_short Eplet-Predicted Antigens: An Attempt to Introduce Eplets into Unacceptable Antigen Determination and Calculated Panel-Reactive Antibody Calculation Facilitating Kidney Allocation
title_sort eplet-predicted antigens: an attempt to introduce eplets into unacceptable antigen determination and calculated panel-reactive antibody calculation facilitating kidney allocation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9776513/
https://www.ncbi.nlm.nih.gov/pubmed/36552990
http://dx.doi.org/10.3390/diagnostics12122983
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