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Power Laws Describe Bacterial Viscoelasticity
[Image: see text] Bacterial cells survive in a wide range of different environments and actively tune their mechanical properties for purposes of growth, movement, division, and nutrition. In Gram-negative bacteria, the cell envelope with its outer membrane and peptidoglycan are the main determinant...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9776528/ https://www.ncbi.nlm.nih.gov/pubmed/36484724 http://dx.doi.org/10.1021/acs.langmuir.2c02172 |
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author | Weber, Andreas Tyrakowski, Daniel Toca-Herrera, José L. |
author_facet | Weber, Andreas Tyrakowski, Daniel Toca-Herrera, José L. |
author_sort | Weber, Andreas |
collection | PubMed |
description | [Image: see text] Bacterial cells survive in a wide range of different environments and actively tune their mechanical properties for purposes of growth, movement, division, and nutrition. In Gram-negative bacteria, the cell envelope with its outer membrane and peptidoglycan are the main determinants of mechanical properties and are common targets for the use of antibiotics. The study of bacterial mechanical properties has shown promise in elucidating a structure–function relationship in bacteria, connecting, shape, mechanics, and biochemistry. In this work, we study frequency and time-dependent viscoelastic properties of E. coli cells by atomic force microscopy (AFM). We perform force cycles, oscillatory microrheology, stress relaxation, and creep experiments, and use power law rheology models to fit the experimental results. All data sets could be fitted with the models and provided power law exponents of 0.01 to 0.1 while showing moduli in the range of a few MPa. We provide evidence for the interchangeability of the properties derived from these four different measurement approaches. |
format | Online Article Text |
id | pubmed-9776528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-97765282022-12-23 Power Laws Describe Bacterial Viscoelasticity Weber, Andreas Tyrakowski, Daniel Toca-Herrera, José L. Langmuir [Image: see text] Bacterial cells survive in a wide range of different environments and actively tune their mechanical properties for purposes of growth, movement, division, and nutrition. In Gram-negative bacteria, the cell envelope with its outer membrane and peptidoglycan are the main determinants of mechanical properties and are common targets for the use of antibiotics. The study of bacterial mechanical properties has shown promise in elucidating a structure–function relationship in bacteria, connecting, shape, mechanics, and biochemistry. In this work, we study frequency and time-dependent viscoelastic properties of E. coli cells by atomic force microscopy (AFM). We perform force cycles, oscillatory microrheology, stress relaxation, and creep experiments, and use power law rheology models to fit the experimental results. All data sets could be fitted with the models and provided power law exponents of 0.01 to 0.1 while showing moduli in the range of a few MPa. We provide evidence for the interchangeability of the properties derived from these four different measurement approaches. American Chemical Society 2022-12-09 2022-12-20 /pmc/articles/PMC9776528/ /pubmed/36484724 http://dx.doi.org/10.1021/acs.langmuir.2c02172 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Weber, Andreas Tyrakowski, Daniel Toca-Herrera, José L. Power Laws Describe Bacterial Viscoelasticity |
title | Power Laws
Describe Bacterial Viscoelasticity |
title_full | Power Laws
Describe Bacterial Viscoelasticity |
title_fullStr | Power Laws
Describe Bacterial Viscoelasticity |
title_full_unstemmed | Power Laws
Describe Bacterial Viscoelasticity |
title_short | Power Laws
Describe Bacterial Viscoelasticity |
title_sort | power laws
describe bacterial viscoelasticity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9776528/ https://www.ncbi.nlm.nih.gov/pubmed/36484724 http://dx.doi.org/10.1021/acs.langmuir.2c02172 |
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