Cargando…

Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets

Chemokines are molecules that pertain to a family of small cytokines and can generate cell chemotaxis through the interaction with their receptors. Chemokines can trigger signaling via conventional G-protein-coupled receptors or through atypical chemokine receptors. Currently, four atypical chemokin...

Descripción completa

Detalles Bibliográficos
Autores principales: Pacheco, Messias Oliveira, Rocha, Fernanda Agostini, Aloia, Thiago Pinheiro Arrais, Marti, Luciana Cavalheiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9776531/
https://www.ncbi.nlm.nih.gov/pubmed/36552863
http://dx.doi.org/10.3390/cells11244099
_version_ 1784855888565108736
author Pacheco, Messias Oliveira
Rocha, Fernanda Agostini
Aloia, Thiago Pinheiro Arrais
Marti, Luciana Cavalheiro
author_facet Pacheco, Messias Oliveira
Rocha, Fernanda Agostini
Aloia, Thiago Pinheiro Arrais
Marti, Luciana Cavalheiro
author_sort Pacheco, Messias Oliveira
collection PubMed
description Chemokines are molecules that pertain to a family of small cytokines and can generate cell chemotaxis through the interaction with their receptors. Chemokines can trigger signaling via conventional G-protein-coupled receptors or through atypical chemokine receptors. Currently, four atypical chemokine receptors have been are described (ACKR1, ACKR2, ACKR3 and ACKR4). ACKRs are expressed in various cells and tissues, including T lymphocytes. These receptors’ main function is related to the internalization and degradation of chemokines, as well as to the inflammation control. However, the expression of these receptors in human T lymphocytes is unclear in the literature. The objective of this study was to evaluate the expression of ACKRs in different subpopulations of T lymphocytes. For this, peripheral blood from healthy donors was used to analyze the expression of ACKR2, ACKR3 and ACKR4 by immunophenotyping CD4, CD8 T lymphocytes and, in their subsets, naive, transition and memory. Results obtained in this study demonstrated that ACKR2, ACKR3 and ACKR4 receptors were expressed by T lymphocytes subsets in different proportions. These receptors are highly expressed in the cytoplasmic milieu of all subsets of T lymphocytes, therefore suggesting that their expression in plasma membrane is regulated after transcription, and it must be dependent on a stimulus, which was not identified in our study. Thus, regarding ACKRs function as scavenger receptors, at least for the ACKR3, this function does not impair the chemotaxis exert for their ligand compared to the typical counterpart receptor.
format Online
Article
Text
id pubmed-9776531
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97765312022-12-23 Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets Pacheco, Messias Oliveira Rocha, Fernanda Agostini Aloia, Thiago Pinheiro Arrais Marti, Luciana Cavalheiro Cells Article Chemokines are molecules that pertain to a family of small cytokines and can generate cell chemotaxis through the interaction with their receptors. Chemokines can trigger signaling via conventional G-protein-coupled receptors or through atypical chemokine receptors. Currently, four atypical chemokine receptors have been are described (ACKR1, ACKR2, ACKR3 and ACKR4). ACKRs are expressed in various cells and tissues, including T lymphocytes. These receptors’ main function is related to the internalization and degradation of chemokines, as well as to the inflammation control. However, the expression of these receptors in human T lymphocytes is unclear in the literature. The objective of this study was to evaluate the expression of ACKRs in different subpopulations of T lymphocytes. For this, peripheral blood from healthy donors was used to analyze the expression of ACKR2, ACKR3 and ACKR4 by immunophenotyping CD4, CD8 T lymphocytes and, in their subsets, naive, transition and memory. Results obtained in this study demonstrated that ACKR2, ACKR3 and ACKR4 receptors were expressed by T lymphocytes subsets in different proportions. These receptors are highly expressed in the cytoplasmic milieu of all subsets of T lymphocytes, therefore suggesting that their expression in plasma membrane is regulated after transcription, and it must be dependent on a stimulus, which was not identified in our study. Thus, regarding ACKRs function as scavenger receptors, at least for the ACKR3, this function does not impair the chemotaxis exert for their ligand compared to the typical counterpart receptor. MDPI 2022-12-16 /pmc/articles/PMC9776531/ /pubmed/36552863 http://dx.doi.org/10.3390/cells11244099 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pacheco, Messias Oliveira
Rocha, Fernanda Agostini
Aloia, Thiago Pinheiro Arrais
Marti, Luciana Cavalheiro
Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets
title Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets
title_full Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets
title_fullStr Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets
title_full_unstemmed Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets
title_short Evaluation of Atypical Chemokine Receptor Expression in T Cell Subsets
title_sort evaluation of atypical chemokine receptor expression in t cell subsets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9776531/
https://www.ncbi.nlm.nih.gov/pubmed/36552863
http://dx.doi.org/10.3390/cells11244099
work_keys_str_mv AT pachecomessiasoliveira evaluationofatypicalchemokinereceptorexpressionintcellsubsets
AT rochafernandaagostini evaluationofatypicalchemokinereceptorexpressionintcellsubsets
AT aloiathiagopinheiroarrais evaluationofatypicalchemokinereceptorexpressionintcellsubsets
AT martilucianacavalheiro evaluationofatypicalchemokinereceptorexpressionintcellsubsets