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Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths

Helminth infections caused by nematodes, trematodes, and cestodes are major neglected tropical diseases and of great medical and veterinary relevance. At present, diagnosis of helminthic diseases is mainly based on microscopic observation of different parasite stages, but microscopy is associated wi...

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Detalles Bibliográficos
Autores principales: Sy, Issa, Conrad, Lucie, Becker, Sören L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777230/
https://www.ncbi.nlm.nih.gov/pubmed/36553043
http://dx.doi.org/10.3390/diagnostics12123035
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author Sy, Issa
Conrad, Lucie
Becker, Sören L.
author_facet Sy, Issa
Conrad, Lucie
Becker, Sören L.
author_sort Sy, Issa
collection PubMed
description Helminth infections caused by nematodes, trematodes, and cestodes are major neglected tropical diseases and of great medical and veterinary relevance. At present, diagnosis of helminthic diseases is mainly based on microscopic observation of different parasite stages, but microscopy is associated with limited diagnostic accuracy. Against this background, recent studies described matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry as a potential, innovative tool for helminth identification and differentiation. MALDI-TOF mass spectrometry is based on the analysis of spectra profiles generated from protein extracts of a given pathogen. It requires an available spectra database containing reference spectra, also called main spectra profiles (MSPs), which are generated from well characterized specimens. At present, however, there are no commercially available databases for helminth identification using this approach. In this narrative review, we summarize recent developments and published studies between January 2019 and September 2022 that report on the use of MALDI-TOF mass spectrometry for helminths. Current challenges and future research needs are identified and briefly discussed.
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spelling pubmed-97772302022-12-23 Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths Sy, Issa Conrad, Lucie Becker, Sören L. Diagnostics (Basel) Review Helminth infections caused by nematodes, trematodes, and cestodes are major neglected tropical diseases and of great medical and veterinary relevance. At present, diagnosis of helminthic diseases is mainly based on microscopic observation of different parasite stages, but microscopy is associated with limited diagnostic accuracy. Against this background, recent studies described matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry as a potential, innovative tool for helminth identification and differentiation. MALDI-TOF mass spectrometry is based on the analysis of spectra profiles generated from protein extracts of a given pathogen. It requires an available spectra database containing reference spectra, also called main spectra profiles (MSPs), which are generated from well characterized specimens. At present, however, there are no commercially available databases for helminth identification using this approach. In this narrative review, we summarize recent developments and published studies between January 2019 and September 2022 that report on the use of MALDI-TOF mass spectrometry for helminths. Current challenges and future research needs are identified and briefly discussed. MDPI 2022-12-03 /pmc/articles/PMC9777230/ /pubmed/36553043 http://dx.doi.org/10.3390/diagnostics12123035 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sy, Issa
Conrad, Lucie
Becker, Sören L.
Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths
title Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths
title_full Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths
title_fullStr Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths
title_full_unstemmed Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths
title_short Recent Advances and Potential Future Applications of MALDI-TOF Mass Spectrometry for Identification of Helminths
title_sort recent advances and potential future applications of maldi-tof mass spectrometry for identification of helminths
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777230/
https://www.ncbi.nlm.nih.gov/pubmed/36553043
http://dx.doi.org/10.3390/diagnostics12123035
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