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The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development

Foot-and-mouth disease virus (FMDV) poses a significant threat to the livestock industry. Through their recognition of the conserved epitopes presented by the swine leukocyte antigen (SLA), T cells play a pivotal role in the antiviral immunity of pigs. Herein, based on the peptide binding motif of S...

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Autores principales: Feng, Lei, Gao, Yong-Yu, Sun, Mingwei, Li, Zi-Bin, Zhang, Qiang, Yang, Jie, Qiao, Cui, Jin, Hang, Feng, Hong-Sheng, Xian, Yu-Han, Qi, Jianxun, Gao, George F., Liu, William J., Gao, Feng-Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777387/
https://www.ncbi.nlm.nih.gov/pubmed/36552780
http://dx.doi.org/10.3390/cells11244017
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author Feng, Lei
Gao, Yong-Yu
Sun, Mingwei
Li, Zi-Bin
Zhang, Qiang
Yang, Jie
Qiao, Cui
Jin, Hang
Feng, Hong-Sheng
Xian, Yu-Han
Qi, Jianxun
Gao, George F.
Liu, William J.
Gao, Feng-Shan
author_facet Feng, Lei
Gao, Yong-Yu
Sun, Mingwei
Li, Zi-Bin
Zhang, Qiang
Yang, Jie
Qiao, Cui
Jin, Hang
Feng, Hong-Sheng
Xian, Yu-Han
Qi, Jianxun
Gao, George F.
Liu, William J.
Gao, Feng-Shan
author_sort Feng, Lei
collection PubMed
description Foot-and-mouth disease virus (FMDV) poses a significant threat to the livestock industry. Through their recognition of the conserved epitopes presented by the swine leukocyte antigen (SLA), T cells play a pivotal role in the antiviral immunity of pigs. Herein, based on the peptide binding motif of SLA-2*HB01, from an original SLA-2 allele, a series of functional T-cell epitopes derived from the dominant antigen VP1 of FMDV with high binding capacity to SLA-2 were identified. Two parallel peptides, Hu64 and As64, from the O and Asia I serotypes, respectively, were both crystallized with SLA-2*HB01. Compared to SLA-1 and SLA-3, the SLA-2 structures showed the flexibility of residues in the P4, P6, and P8 positions and in their potential interface with TCR. Notably, the peptides Hu64 and As64 adopted quite similar overall conformation when bound to SLA-2*HB01. Hu64 has two different conformations, a more stable ‘chair’ conformation and an unstable ‘boat’ conformation observed in the two molecules of one asymmetric unit, whereas only a single ‘chair’ conformation was observed for As64. Both Hu64 and As64 could induce similar dominant T-cell activities. Our interdisciplinary study establishes a basis for the in-depth interpretation of the peptide presentation of SLA-I, which can be used toward the development of universal vaccines.
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spelling pubmed-97773872022-12-23 The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development Feng, Lei Gao, Yong-Yu Sun, Mingwei Li, Zi-Bin Zhang, Qiang Yang, Jie Qiao, Cui Jin, Hang Feng, Hong-Sheng Xian, Yu-Han Qi, Jianxun Gao, George F. Liu, William J. Gao, Feng-Shan Cells Article Foot-and-mouth disease virus (FMDV) poses a significant threat to the livestock industry. Through their recognition of the conserved epitopes presented by the swine leukocyte antigen (SLA), T cells play a pivotal role in the antiviral immunity of pigs. Herein, based on the peptide binding motif of SLA-2*HB01, from an original SLA-2 allele, a series of functional T-cell epitopes derived from the dominant antigen VP1 of FMDV with high binding capacity to SLA-2 were identified. Two parallel peptides, Hu64 and As64, from the O and Asia I serotypes, respectively, were both crystallized with SLA-2*HB01. Compared to SLA-1 and SLA-3, the SLA-2 structures showed the flexibility of residues in the P4, P6, and P8 positions and in their potential interface with TCR. Notably, the peptides Hu64 and As64 adopted quite similar overall conformation when bound to SLA-2*HB01. Hu64 has two different conformations, a more stable ‘chair’ conformation and an unstable ‘boat’ conformation observed in the two molecules of one asymmetric unit, whereas only a single ‘chair’ conformation was observed for As64. Both Hu64 and As64 could induce similar dominant T-cell activities. Our interdisciplinary study establishes a basis for the in-depth interpretation of the peptide presentation of SLA-I, which can be used toward the development of universal vaccines. MDPI 2022-12-12 /pmc/articles/PMC9777387/ /pubmed/36552780 http://dx.doi.org/10.3390/cells11244017 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Feng, Lei
Gao, Yong-Yu
Sun, Mingwei
Li, Zi-Bin
Zhang, Qiang
Yang, Jie
Qiao, Cui
Jin, Hang
Feng, Hong-Sheng
Xian, Yu-Han
Qi, Jianxun
Gao, George F.
Liu, William J.
Gao, Feng-Shan
The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development
title The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development
title_full The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development
title_fullStr The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development
title_full_unstemmed The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development
title_short The Parallel Presentation of Two Functional CTL Epitopes Derived from the O and Asia 1 Serotypes of Foot-and-Mouth Disease Virus and Swine SLA-2*HB01: Implications for Universal Vaccine Development
title_sort parallel presentation of two functional ctl epitopes derived from the o and asia 1 serotypes of foot-and-mouth disease virus and swine sla-2*hb01: implications for universal vaccine development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777387/
https://www.ncbi.nlm.nih.gov/pubmed/36552780
http://dx.doi.org/10.3390/cells11244017
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