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Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa
Clubroot, caused by Plasmodiophora brassicae, is an economically important soil-borne disease that threatens Brassicaceae crops worldwide. In recent years, the incidence area of Chinese cabbage (Brassica rapa ssp. pekinensis) clubroot disease has increased, which severely affects the yield and quali...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777773/ https://www.ncbi.nlm.nih.gov/pubmed/36553679 http://dx.doi.org/10.3390/genes13122414 |
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author | Li, Xiaonan Wei, Yingxia Ma, Yingmei Cao, Guizhu Ma, Siwen Zhang, Tianyu Zhan, Zongxiang Piao, Zhongyun |
author_facet | Li, Xiaonan Wei, Yingxia Ma, Yingmei Cao, Guizhu Ma, Siwen Zhang, Tianyu Zhan, Zongxiang Piao, Zhongyun |
author_sort | Li, Xiaonan |
collection | PubMed |
description | Clubroot, caused by Plasmodiophora brassicae, is an economically important soil-borne disease that threatens Brassicaceae crops worldwide. In recent years, the incidence area of Chinese cabbage (Brassica rapa ssp. pekinensis) clubroot disease has increased, which severely affects the yield and quality of Chinese cabbage. The resistance of varieties harboring the single clubroot-resistance (CR) gene is easily broken through by P. brassicae pathotypes. CRa and CRd, genetically identified in B. rapa, are CR genes known to be highly resistant to different P. brassicaea pathotypes. In our study, we perform the gene pyramiding of CRa and CRd in Chinese cabbages through marker-assisted selection (MAS), and develop homozygous pyramided lines. The newly generated pyramided lines exhibit greater resistance to six different pathotypes than that of two parental lines carrying a single CR gene. This study provides new CR-gene-pyramided lines for the development of clubroot-resistant Brassica varieties for future breeding programs. |
format | Online Article Text |
id | pubmed-9777773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97777732022-12-23 Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa Li, Xiaonan Wei, Yingxia Ma, Yingmei Cao, Guizhu Ma, Siwen Zhang, Tianyu Zhan, Zongxiang Piao, Zhongyun Genes (Basel) Article Clubroot, caused by Plasmodiophora brassicae, is an economically important soil-borne disease that threatens Brassicaceae crops worldwide. In recent years, the incidence area of Chinese cabbage (Brassica rapa ssp. pekinensis) clubroot disease has increased, which severely affects the yield and quality of Chinese cabbage. The resistance of varieties harboring the single clubroot-resistance (CR) gene is easily broken through by P. brassicae pathotypes. CRa and CRd, genetically identified in B. rapa, are CR genes known to be highly resistant to different P. brassicaea pathotypes. In our study, we perform the gene pyramiding of CRa and CRd in Chinese cabbages through marker-assisted selection (MAS), and develop homozygous pyramided lines. The newly generated pyramided lines exhibit greater resistance to six different pathotypes than that of two parental lines carrying a single CR gene. This study provides new CR-gene-pyramided lines for the development of clubroot-resistant Brassica varieties for future breeding programs. MDPI 2022-12-19 /pmc/articles/PMC9777773/ /pubmed/36553679 http://dx.doi.org/10.3390/genes13122414 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Xiaonan Wei, Yingxia Ma, Yingmei Cao, Guizhu Ma, Siwen Zhang, Tianyu Zhan, Zongxiang Piao, Zhongyun Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa |
title | Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa |
title_full | Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa |
title_fullStr | Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa |
title_full_unstemmed | Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa |
title_short | Marker-Assisted Pyramiding of CRa and CRd Genes to Improve the Clubroot Resistance of Brassica rapa |
title_sort | marker-assisted pyramiding of cra and crd genes to improve the clubroot resistance of brassica rapa |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777773/ https://www.ncbi.nlm.nih.gov/pubmed/36553679 http://dx.doi.org/10.3390/genes13122414 |
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