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MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their signific...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778022/ https://www.ncbi.nlm.nih.gov/pubmed/36553586 http://dx.doi.org/10.3390/genes13122320 |
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author | Joković, Suzana Matijašević Dobrijević, Zorana Kotarac, Nevena Filipović, Lidija Popović, Milica Korać, Aleksandra Vuković, Ivan Savić-Pavićević, Dušanka Brajušković, Goran |
author_facet | Joković, Suzana Matijašević Dobrijević, Zorana Kotarac, Nevena Filipović, Lidija Popović, Milica Korać, Aleksandra Vuković, Ivan Savić-Pavićević, Dušanka Brajušković, Goran |
author_sort | Joković, Suzana Matijašević |
collection | PubMed |
description | MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their significance as PCa biomarkers, as well as to compare different types of specimens as a source of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we did not find an association with the values of standard prognostic parameters of PCa, nor with cancer aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21 expression levels in PCa. |
format | Online Article Text |
id | pubmed-9778022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97780222022-12-23 MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes Joković, Suzana Matijašević Dobrijević, Zorana Kotarac, Nevena Filipović, Lidija Popović, Milica Korać, Aleksandra Vuković, Ivan Savić-Pavićević, Dušanka Brajušković, Goran Genes (Basel) Article MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their significance as PCa biomarkers, as well as to compare different types of specimens as a source of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we did not find an association with the values of standard prognostic parameters of PCa, nor with cancer aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21 expression levels in PCa. MDPI 2022-12-09 /pmc/articles/PMC9778022/ /pubmed/36553586 http://dx.doi.org/10.3390/genes13122320 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Joković, Suzana Matijašević Dobrijević, Zorana Kotarac, Nevena Filipović, Lidija Popović, Milica Korać, Aleksandra Vuković, Ivan Savić-Pavićević, Dušanka Brajušković, Goran MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes |
title | MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes |
title_full | MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes |
title_fullStr | MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes |
title_full_unstemmed | MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes |
title_short | MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes |
title_sort | mir-375 and mir-21 as potential biomarkers of prostate cancer: comparison of matching samples of plasma and exosomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778022/ https://www.ncbi.nlm.nih.gov/pubmed/36553586 http://dx.doi.org/10.3390/genes13122320 |
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