Cargando…

MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes

MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their signific...

Descripción completa

Detalles Bibliográficos
Autores principales: Joković, Suzana Matijašević, Dobrijević, Zorana, Kotarac, Nevena, Filipović, Lidija, Popović, Milica, Korać, Aleksandra, Vuković, Ivan, Savić-Pavićević, Dušanka, Brajušković, Goran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778022/
https://www.ncbi.nlm.nih.gov/pubmed/36553586
http://dx.doi.org/10.3390/genes13122320
_version_ 1784856254041030656
author Joković, Suzana Matijašević
Dobrijević, Zorana
Kotarac, Nevena
Filipović, Lidija
Popović, Milica
Korać, Aleksandra
Vuković, Ivan
Savić-Pavićević, Dušanka
Brajušković, Goran
author_facet Joković, Suzana Matijašević
Dobrijević, Zorana
Kotarac, Nevena
Filipović, Lidija
Popović, Milica
Korać, Aleksandra
Vuković, Ivan
Savić-Pavićević, Dušanka
Brajušković, Goran
author_sort Joković, Suzana Matijašević
collection PubMed
description MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their significance as PCa biomarkers, as well as to compare different types of specimens as a source of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we did not find an association with the values of standard prognostic parameters of PCa, nor with cancer aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21 expression levels in PCa.
format Online
Article
Text
id pubmed-9778022
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97780222022-12-23 MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes Joković, Suzana Matijašević Dobrijević, Zorana Kotarac, Nevena Filipović, Lidija Popović, Milica Korać, Aleksandra Vuković, Ivan Savić-Pavićević, Dušanka Brajušković, Goran Genes (Basel) Article MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their significance as PCa biomarkers, as well as to compare different types of specimens as a source of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we did not find an association with the values of standard prognostic parameters of PCa, nor with cancer aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21 expression levels in PCa. MDPI 2022-12-09 /pmc/articles/PMC9778022/ /pubmed/36553586 http://dx.doi.org/10.3390/genes13122320 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Joković, Suzana Matijašević
Dobrijević, Zorana
Kotarac, Nevena
Filipović, Lidija
Popović, Milica
Korać, Aleksandra
Vuković, Ivan
Savić-Pavićević, Dušanka
Brajušković, Goran
MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
title MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
title_full MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
title_fullStr MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
title_full_unstemmed MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
title_short MiR-375 and miR-21 as Potential Biomarkers of Prostate Cancer: Comparison of Matching Samples of Plasma and Exosomes
title_sort mir-375 and mir-21 as potential biomarkers of prostate cancer: comparison of matching samples of plasma and exosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778022/
https://www.ncbi.nlm.nih.gov/pubmed/36553586
http://dx.doi.org/10.3390/genes13122320
work_keys_str_mv AT jokovicsuzanamatijasevic mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT dobrijeviczorana mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT kotaracnevena mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT filipoviclidija mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT popovicmilica mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT koracaleksandra mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT vukovicivan mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT savicpavicevicdusanka mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes
AT brajuskovicgoran mir375andmir21aspotentialbiomarkersofprostatecancercomparisonofmatchingsamplesofplasmaandexosomes