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Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells

Melatonin (MEL) presents well-documented pleiotropic actions against oxidative stress (OS), acting indirectly through activation of transcription factors, e.g., FoxO3 and Nrf2. Thus, this study aimed to investigate the possible modulating effects of MEL on the redox signaling pathways PI3K/AKT/FoxO3...

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Autores principales: Torres, Flaviene Felix, Bernardo, Victoria Simões, de Paula, Carla Peres, da Silva, João Pedro Maia de Oliveira, de Almeida, Eduardo Alves, da Cunha, Anderson Ferreira, da Silva, Danilo Grünig Humberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778059/
https://www.ncbi.nlm.nih.gov/pubmed/36553603
http://dx.doi.org/10.3390/genes13122337
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author Torres, Flaviene Felix
Bernardo, Victoria Simões
de Paula, Carla Peres
da Silva, João Pedro Maia de Oliveira
de Almeida, Eduardo Alves
da Cunha, Anderson Ferreira
da Silva, Danilo Grünig Humberto
author_facet Torres, Flaviene Felix
Bernardo, Victoria Simões
de Paula, Carla Peres
da Silva, João Pedro Maia de Oliveira
de Almeida, Eduardo Alves
da Cunha, Anderson Ferreira
da Silva, Danilo Grünig Humberto
author_sort Torres, Flaviene Felix
collection PubMed
description Melatonin (MEL) presents well-documented pleiotropic actions against oxidative stress (OS), acting indirectly through activation of transcription factors, e.g., FoxO3 and Nrf2. Thus, this study aimed to investigate the possible modulating effects of MEL on the redox signaling pathways PI3K/AKT/FoxO3 and Keap1/Nrf2/ARE in K562 erythroleukemic cells subjected to OS induction. For this, the viability, and transcript levels of genes involved in redox adaptation were evaluated in K562 cells in different periods of erythroid differentiation: under OS induction by hydrogen peroxide (100 µM H(2)O(2)); treated with 1 nM (C1) and 1 mM (C2) MEL; and associated or not with stress induction. We observed a restoration of physiological levels of Nrf2 in both MEL concentrations under OS. The C1 was related to enhanced expression of antioxidant and proteasome genes through the Nrf2-ARE pathway, while C2 to the induction of FOXO3 expression, suggesting an involvement with apoptotic pathway, according to BIM transcript levels. The effects of MEL administration in these cells showed a period and dose-dependent pattern against induced-OS, with direct and indirect actions through different pathways of cellular adaptation, reinforcing the importance of this indolamine in the regulation of cellular homeostasis, being a promising therapeutic alternative for diseases that present an exacerbated OS.
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spelling pubmed-97780592022-12-23 Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells Torres, Flaviene Felix Bernardo, Victoria Simões de Paula, Carla Peres da Silva, João Pedro Maia de Oliveira de Almeida, Eduardo Alves da Cunha, Anderson Ferreira da Silva, Danilo Grünig Humberto Genes (Basel) Article Melatonin (MEL) presents well-documented pleiotropic actions against oxidative stress (OS), acting indirectly through activation of transcription factors, e.g., FoxO3 and Nrf2. Thus, this study aimed to investigate the possible modulating effects of MEL on the redox signaling pathways PI3K/AKT/FoxO3 and Keap1/Nrf2/ARE in K562 erythroleukemic cells subjected to OS induction. For this, the viability, and transcript levels of genes involved in redox adaptation were evaluated in K562 cells in different periods of erythroid differentiation: under OS induction by hydrogen peroxide (100 µM H(2)O(2)); treated with 1 nM (C1) and 1 mM (C2) MEL; and associated or not with stress induction. We observed a restoration of physiological levels of Nrf2 in both MEL concentrations under OS. The C1 was related to enhanced expression of antioxidant and proteasome genes through the Nrf2-ARE pathway, while C2 to the induction of FOXO3 expression, suggesting an involvement with apoptotic pathway, according to BIM transcript levels. The effects of MEL administration in these cells showed a period and dose-dependent pattern against induced-OS, with direct and indirect actions through different pathways of cellular adaptation, reinforcing the importance of this indolamine in the regulation of cellular homeostasis, being a promising therapeutic alternative for diseases that present an exacerbated OS. MDPI 2022-12-11 /pmc/articles/PMC9778059/ /pubmed/36553603 http://dx.doi.org/10.3390/genes13122337 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Torres, Flaviene Felix
Bernardo, Victoria Simões
de Paula, Carla Peres
da Silva, João Pedro Maia de Oliveira
de Almeida, Eduardo Alves
da Cunha, Anderson Ferreira
da Silva, Danilo Grünig Humberto
Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells
title Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells
title_full Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells
title_fullStr Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells
title_full_unstemmed Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells
title_short Influence of Melatonin Treatment on Cellular Mechanisms of Redox Adaptation in K562 Erythroleukemic Cells
title_sort influence of melatonin treatment on cellular mechanisms of redox adaptation in k562 erythroleukemic cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778059/
https://www.ncbi.nlm.nih.gov/pubmed/36553603
http://dx.doi.org/10.3390/genes13122337
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