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Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging

This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in Arctium lappa L. (A. lappa) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabo...

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Detalles Bibliográficos
Autores principales: Li, Lingyu, Qiu, Zhichang, Jiang, Mingdi, Zhang, Bin, Chen, Qiang, Zhang, Chaojie, Zheng, Zhenjia, Qiao, Xuguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778511/
https://www.ncbi.nlm.nih.gov/pubmed/36553700
http://dx.doi.org/10.3390/foods11243957
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author Li, Lingyu
Qiu, Zhichang
Jiang, Mingdi
Zhang, Bin
Chen, Qiang
Zhang, Chaojie
Zheng, Zhenjia
Qiao, Xuguang
author_facet Li, Lingyu
Qiu, Zhichang
Jiang, Mingdi
Zhang, Bin
Chen, Qiang
Zhang, Chaojie
Zheng, Zhenjia
Qiao, Xuguang
author_sort Li, Lingyu
collection PubMed
description This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in Arctium lappa L. (A. lappa) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabolism process. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) imaging technology was used for visual demonstration of the in situ spatial distribution in A. lappa roots. This work consisted of four steps: matrix selection, section preparation, matrix coating, and MALDI-TOF MS imaging analysis. Consequently, eight saccharides, four caffeoylquinic acids, four flavonoids, six amino acids, one choline, and one phospholipid were imaged and four unidentified components were found. Saccharides were distributed in the center, whereas caffeoylquinic acids and flavonoids were mainly present in the epidermis and cortex. Furthermore, amino acids were mainly detected in the phloem, and choline in the cambium, while phosphatidylserine was found in the secondary phloem and cambium. This study demonstrated that MALDI-TOF MS imaging technology could provide a technical support to understand the spatial distribution of components in A. lappa roots, which would promote the processing technologies for A. lappa roots and help us to understand the plant metabolism process.
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spelling pubmed-97785112022-12-23 Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging Li, Lingyu Qiu, Zhichang Jiang, Mingdi Zhang, Bin Chen, Qiang Zhang, Chaojie Zheng, Zhenjia Qiao, Xuguang Foods Article This study is aimed at developing novel analytical methods to accurately visualize the spatial distribution of various endogenous components in Arctium lappa L. (A. lappa) roots, and to precisely guide the setting of pre-treatment operations during processing technologies and understand plant metabolism process. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) imaging technology was used for visual demonstration of the in situ spatial distribution in A. lappa roots. This work consisted of four steps: matrix selection, section preparation, matrix coating, and MALDI-TOF MS imaging analysis. Consequently, eight saccharides, four caffeoylquinic acids, four flavonoids, six amino acids, one choline, and one phospholipid were imaged and four unidentified components were found. Saccharides were distributed in the center, whereas caffeoylquinic acids and flavonoids were mainly present in the epidermis and cortex. Furthermore, amino acids were mainly detected in the phloem, and choline in the cambium, while phosphatidylserine was found in the secondary phloem and cambium. This study demonstrated that MALDI-TOF MS imaging technology could provide a technical support to understand the spatial distribution of components in A. lappa roots, which would promote the processing technologies for A. lappa roots and help us to understand the plant metabolism process. MDPI 2022-12-07 /pmc/articles/PMC9778511/ /pubmed/36553700 http://dx.doi.org/10.3390/foods11243957 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Lingyu
Qiu, Zhichang
Jiang, Mingdi
Zhang, Bin
Chen, Qiang
Zhang, Chaojie
Zheng, Zhenjia
Qiao, Xuguang
Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging
title Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging
title_full Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging
title_fullStr Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging
title_full_unstemmed Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging
title_short Visualizing the Spatial Distribution of Arctium lappa L. Root Components by MALDI-TOF Mass Spectrometry Imaging
title_sort visualizing the spatial distribution of arctium lappa l. root components by maldi-tof mass spectrometry imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778511/
https://www.ncbi.nlm.nih.gov/pubmed/36553700
http://dx.doi.org/10.3390/foods11243957
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