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Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterogeneity in analytical procedure, wastewater volume,...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778686/ https://www.ncbi.nlm.nih.gov/pubmed/36554466 http://dx.doi.org/10.3390/ijerph192416588 |
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author | Verani, Marco Federigi, Ileana Muzio, Sara Lauretani, Giulia Calà, Piergiuseppe Mancuso, Fabrizio Salvadori, Roberto Valentini, Claudia La Rosa, Giuseppina Suffredini, Elisabetta Carducci, Annalaura |
author_facet | Verani, Marco Federigi, Ileana Muzio, Sara Lauretani, Giulia Calà, Piergiuseppe Mancuso, Fabrizio Salvadori, Roberto Valentini, Claudia La Rosa, Giuseppina Suffredini, Elisabetta Carducci, Annalaura |
author_sort | Verani, Marco |
collection | PubMed |
description | The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterogeneity in analytical procedure, wastewater volume, and population size. In this paper, raw sewage SARS-CoV-2 samples were collected from four wastewater treatment plants (WWTPs) in Tuscany (Northwest Italy) between February and December 2021. During the surveillance period, viral concentration was based on polyethylene glycol (PEG), but its precipitation method was modified from biphasic separation to centrifugation. Therefore, in parallel, the recovery efficiency of each method was evaluated at lab-scale, using two spiking viruses (human coronavirus 229E and mengovirus vMC(0)). SARS-CoV-2 genome was found in 80 (46.5%) of the 172 examined samples. Lab-scale experiments revealed that PEG precipitation using centrifugation had the best recovery efficiency (up to 30%). Viral SARS-CoV-2 load obtained from sewage data, adjusted by analytical method and normalized by population of each WWTP, showed a good association with the clinical data in the study area. This study highlights that environmental surveillance data need to be carefully analyzed before their use in the WBE, also considering the sensibility of the analytical methods. |
format | Online Article Text |
id | pubmed-9778686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97786862022-12-23 Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany Verani, Marco Federigi, Ileana Muzio, Sara Lauretani, Giulia Calà, Piergiuseppe Mancuso, Fabrizio Salvadori, Roberto Valentini, Claudia La Rosa, Giuseppina Suffredini, Elisabetta Carducci, Annalaura Int J Environ Res Public Health Article The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterogeneity in analytical procedure, wastewater volume, and population size. In this paper, raw sewage SARS-CoV-2 samples were collected from four wastewater treatment plants (WWTPs) in Tuscany (Northwest Italy) between February and December 2021. During the surveillance period, viral concentration was based on polyethylene glycol (PEG), but its precipitation method was modified from biphasic separation to centrifugation. Therefore, in parallel, the recovery efficiency of each method was evaluated at lab-scale, using two spiking viruses (human coronavirus 229E and mengovirus vMC(0)). SARS-CoV-2 genome was found in 80 (46.5%) of the 172 examined samples. Lab-scale experiments revealed that PEG precipitation using centrifugation had the best recovery efficiency (up to 30%). Viral SARS-CoV-2 load obtained from sewage data, adjusted by analytical method and normalized by population of each WWTP, showed a good association with the clinical data in the study area. This study highlights that environmental surveillance data need to be carefully analyzed before their use in the WBE, also considering the sensibility of the analytical methods. MDPI 2022-12-09 /pmc/articles/PMC9778686/ /pubmed/36554466 http://dx.doi.org/10.3390/ijerph192416588 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Verani, Marco Federigi, Ileana Muzio, Sara Lauretani, Giulia Calà, Piergiuseppe Mancuso, Fabrizio Salvadori, Roberto Valentini, Claudia La Rosa, Giuseppina Suffredini, Elisabetta Carducci, Annalaura Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany |
title | Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany |
title_full | Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany |
title_fullStr | Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany |
title_full_unstemmed | Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany |
title_short | Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany |
title_sort | calibration of methods for sars-cov-2 environmental surveillance: a case study from northwest tuscany |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778686/ https://www.ncbi.nlm.nih.gov/pubmed/36554466 http://dx.doi.org/10.3390/ijerph192416588 |
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