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Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany

The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterogeneity in analytical procedure, wastewater volume,...

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Autores principales: Verani, Marco, Federigi, Ileana, Muzio, Sara, Lauretani, Giulia, Calà, Piergiuseppe, Mancuso, Fabrizio, Salvadori, Roberto, Valentini, Claudia, La Rosa, Giuseppina, Suffredini, Elisabetta, Carducci, Annalaura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778686/
https://www.ncbi.nlm.nih.gov/pubmed/36554466
http://dx.doi.org/10.3390/ijerph192416588
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author Verani, Marco
Federigi, Ileana
Muzio, Sara
Lauretani, Giulia
Calà, Piergiuseppe
Mancuso, Fabrizio
Salvadori, Roberto
Valentini, Claudia
La Rosa, Giuseppina
Suffredini, Elisabetta
Carducci, Annalaura
author_facet Verani, Marco
Federigi, Ileana
Muzio, Sara
Lauretani, Giulia
Calà, Piergiuseppe
Mancuso, Fabrizio
Salvadori, Roberto
Valentini, Claudia
La Rosa, Giuseppina
Suffredini, Elisabetta
Carducci, Annalaura
author_sort Verani, Marco
collection PubMed
description The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterogeneity in analytical procedure, wastewater volume, and population size. In this paper, raw sewage SARS-CoV-2 samples were collected from four wastewater treatment plants (WWTPs) in Tuscany (Northwest Italy) between February and December 2021. During the surveillance period, viral concentration was based on polyethylene glycol (PEG), but its precipitation method was modified from biphasic separation to centrifugation. Therefore, in parallel, the recovery efficiency of each method was evaluated at lab-scale, using two spiking viruses (human coronavirus 229E and mengovirus vMC(0)). SARS-CoV-2 genome was found in 80 (46.5%) of the 172 examined samples. Lab-scale experiments revealed that PEG precipitation using centrifugation had the best recovery efficiency (up to 30%). Viral SARS-CoV-2 load obtained from sewage data, adjusted by analytical method and normalized by population of each WWTP, showed a good association with the clinical data in the study area. This study highlights that environmental surveillance data need to be carefully analyzed before their use in the WBE, also considering the sensibility of the analytical methods.
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spelling pubmed-97786862022-12-23 Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany Verani, Marco Federigi, Ileana Muzio, Sara Lauretani, Giulia Calà, Piergiuseppe Mancuso, Fabrizio Salvadori, Roberto Valentini, Claudia La Rosa, Giuseppina Suffredini, Elisabetta Carducci, Annalaura Int J Environ Res Public Health Article The current pandemic has provided an opportunity to test wastewater-based epidemiology (WBE) as a complementary method to SARS-CoV-2 monitoring in the community. However, WBE infection estimates can be affected by uncertainty factors, such as heterogeneity in analytical procedure, wastewater volume, and population size. In this paper, raw sewage SARS-CoV-2 samples were collected from four wastewater treatment plants (WWTPs) in Tuscany (Northwest Italy) between February and December 2021. During the surveillance period, viral concentration was based on polyethylene glycol (PEG), but its precipitation method was modified from biphasic separation to centrifugation. Therefore, in parallel, the recovery efficiency of each method was evaluated at lab-scale, using two spiking viruses (human coronavirus 229E and mengovirus vMC(0)). SARS-CoV-2 genome was found in 80 (46.5%) of the 172 examined samples. Lab-scale experiments revealed that PEG precipitation using centrifugation had the best recovery efficiency (up to 30%). Viral SARS-CoV-2 load obtained from sewage data, adjusted by analytical method and normalized by population of each WWTP, showed a good association with the clinical data in the study area. This study highlights that environmental surveillance data need to be carefully analyzed before their use in the WBE, also considering the sensibility of the analytical methods. MDPI 2022-12-09 /pmc/articles/PMC9778686/ /pubmed/36554466 http://dx.doi.org/10.3390/ijerph192416588 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Verani, Marco
Federigi, Ileana
Muzio, Sara
Lauretani, Giulia
Calà, Piergiuseppe
Mancuso, Fabrizio
Salvadori, Roberto
Valentini, Claudia
La Rosa, Giuseppina
Suffredini, Elisabetta
Carducci, Annalaura
Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
title Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
title_full Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
title_fullStr Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
title_full_unstemmed Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
title_short Calibration of Methods for SARS-CoV-2 Environmental Surveillance: A Case Study from Northwest Tuscany
title_sort calibration of methods for sars-cov-2 environmental surveillance: a case study from northwest tuscany
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778686/
https://www.ncbi.nlm.nih.gov/pubmed/36554466
http://dx.doi.org/10.3390/ijerph192416588
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