Cargando…

Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells

Long noncoding RNAs (lncRNAs) are distributed in various species and play critical roles in plant growth, development, and defence against stimuli. However, the lncRNA response to methyl jasmonate (MeJA) treatment has not been well characterized in Nicotiana tabacum Bright Yellow-2 (BY-2) cells, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Kaifeng, Wang, Zitao, Pang, Lu, Song, Zhongbang, Zhao, Heping, Wang, Yingdian, Wang, Bingwu, Han, Shengcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778826/
https://www.ncbi.nlm.nih.gov/pubmed/36555209
http://dx.doi.org/10.3390/ijms232415568
_version_ 1784856458740891648
author Zheng, Kaifeng
Wang, Zitao
Pang, Lu
Song, Zhongbang
Zhao, Heping
Wang, Yingdian
Wang, Bingwu
Han, Shengcheng
author_facet Zheng, Kaifeng
Wang, Zitao
Pang, Lu
Song, Zhongbang
Zhao, Heping
Wang, Yingdian
Wang, Bingwu
Han, Shengcheng
author_sort Zheng, Kaifeng
collection PubMed
description Long noncoding RNAs (lncRNAs) are distributed in various species and play critical roles in plant growth, development, and defence against stimuli. However, the lncRNA response to methyl jasmonate (MeJA) treatment has not been well characterized in Nicotiana tabacum Bright Yellow-2 (BY-2) cells, and their roles in plant defence remain elusive. Here, 7848 reliably expressed lncRNAs were identified in BY-2 cells, of which 629 differentially expressed (DE) lncRNAs were characterized as MeJA-responsive lncRNAs. The lncRNAs in BY-2 cells had a strong genus specificity in Nicotiana. The combined analysis of the cis-regulated lncRNAs and their target genes revealed the potential up- and downregulated target genes that are responsible for different biological functions and metabolic patterns. In addition, some lncRNAs for response-associated target genes might be involved in plant defence and stress resistance via their MeJA- and defence-related cis-regulatory elements. Moreover, some MeJA-responsive lncRNA target genes were related to quinolinate phosphoribosyltransferase, lipoxygenases, and endopeptidase inhibitors, which may contribute to nicotine synthesis and disease and insect resistance, indicating that MeJA-responsive lncRNAs regulate nicotine biosynthesis and disease resistance by regulating their potential target genes in BY-2 cells. Therefore, our results provide more targets for genetically engineering the nicotine content and plant defence in tobacco plants.
format Online
Article
Text
id pubmed-9778826
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97788262022-12-23 Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells Zheng, Kaifeng Wang, Zitao Pang, Lu Song, Zhongbang Zhao, Heping Wang, Yingdian Wang, Bingwu Han, Shengcheng Int J Mol Sci Article Long noncoding RNAs (lncRNAs) are distributed in various species and play critical roles in plant growth, development, and defence against stimuli. However, the lncRNA response to methyl jasmonate (MeJA) treatment has not been well characterized in Nicotiana tabacum Bright Yellow-2 (BY-2) cells, and their roles in plant defence remain elusive. Here, 7848 reliably expressed lncRNAs were identified in BY-2 cells, of which 629 differentially expressed (DE) lncRNAs were characterized as MeJA-responsive lncRNAs. The lncRNAs in BY-2 cells had a strong genus specificity in Nicotiana. The combined analysis of the cis-regulated lncRNAs and their target genes revealed the potential up- and downregulated target genes that are responsible for different biological functions and metabolic patterns. In addition, some lncRNAs for response-associated target genes might be involved in plant defence and stress resistance via their MeJA- and defence-related cis-regulatory elements. Moreover, some MeJA-responsive lncRNA target genes were related to quinolinate phosphoribosyltransferase, lipoxygenases, and endopeptidase inhibitors, which may contribute to nicotine synthesis and disease and insect resistance, indicating that MeJA-responsive lncRNAs regulate nicotine biosynthesis and disease resistance by regulating their potential target genes in BY-2 cells. Therefore, our results provide more targets for genetically engineering the nicotine content and plant defence in tobacco plants. MDPI 2022-12-08 /pmc/articles/PMC9778826/ /pubmed/36555209 http://dx.doi.org/10.3390/ijms232415568 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Kaifeng
Wang, Zitao
Pang, Lu
Song, Zhongbang
Zhao, Heping
Wang, Yingdian
Wang, Bingwu
Han, Shengcheng
Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells
title Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells
title_full Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells
title_fullStr Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells
title_full_unstemmed Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells
title_short Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells
title_sort systematic identification of methyl jasmonate-responsive long noncoding rnas and their nearby coding genes unveils their potential defence roles in tobacco by-2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778826/
https://www.ncbi.nlm.nih.gov/pubmed/36555209
http://dx.doi.org/10.3390/ijms232415568
work_keys_str_mv AT zhengkaifeng systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT wangzitao systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT panglu systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT songzhongbang systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT zhaoheping systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT wangyingdian systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT wangbingwu systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells
AT hanshengcheng systematicidentificationofmethyljasmonateresponsivelongnoncodingrnasandtheirnearbycodinggenesunveilstheirpotentialdefencerolesintobaccoby2cells