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Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice

CRISPR-Cas9-mediated leptin (Lep) knockout (KO) mice exhibited prominent phenotypes for constipation, even though they were not compared with other model animals. This study compared the stool excretion, gastrointestinal motility, histological structure, mucin secretion, and enteric nerve function i...

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Autores principales: Kim, Ji Eun, Seol, Ayun, Choi, Yun Ju, Lee, Su Jin, Jin, You Jeong, Roh, Yu Jeong, Song, Hee Jin, Hong, Jin Tae, Hwang, Dae Youn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778951/
https://www.ncbi.nlm.nih.gov/pubmed/36548335
http://dx.doi.org/10.1371/journal.pone.0276445
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author Kim, Ji Eun
Seol, Ayun
Choi, Yun Ju
Lee, Su Jin
Jin, You Jeong
Roh, Yu Jeong
Song, Hee Jin
Hong, Jin Tae
Hwang, Dae Youn
author_facet Kim, Ji Eun
Seol, Ayun
Choi, Yun Ju
Lee, Su Jin
Jin, You Jeong
Roh, Yu Jeong
Song, Hee Jin
Hong, Jin Tae
Hwang, Dae Youn
author_sort Kim, Ji Eun
collection PubMed
description CRISPR-Cas9-mediated leptin (Lep) knockout (KO) mice exhibited prominent phenotypes for constipation, even though they were not compared with other model animals. This study compared the stool excretion, gastrointestinal motility, histological structure, mucin secretion, and enteric nerve function in Lep KO and high fat diet (HFD)-treated mice to determine if there were differences in their phenotypes for constipation. Most obesity phenotypes, including fat weight, adipocyte size, expression of lipolytic proteins (HSL, perilipin, and ATGL), and glucose concentrations, were detected similarly in the Lep KO and HFD-treated mice. They showed a similar decrease in the excretion parameters, including the stool number, weight, and water content, while the same pattern was detected in the gastrointestinal motility and intestinal length. A similar decrease in the mucosal layer thickness, muscle thickness, ability for mucin secretion, and expression of water channel (aquaporin 3 and 8) genes was detected in the mid-colon of the Lep KO and HFD-treated mice, but the alteration rate in some levels was greater in the HFD-treated group than the Lep KO mice. On the other hand, the levels of c-kit, nNOS, NSE, and PGP9.5 expression for the enteric neurons and intestitial cells of Cajal (ICC) were remarkably lower in the mid-colon of the HFD-treated mice than in the Lep KO mice, but the level of most proteins in both groups remained lower than those in the control group. A similar alteration pattern in the expression of muscarinic acetylcholine receptors (mAChRs) and serotonin receptors was detected in the Lep KO and HFD-treated mice. These results suggest that most phenotypes for obesity-induced constipation were similarly detected in the Lep KO and HFD-treated mice, but there was a difference in the regulatory function of the enteric nervous system (ENS).
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spelling pubmed-97789512022-12-23 Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice Kim, Ji Eun Seol, Ayun Choi, Yun Ju Lee, Su Jin Jin, You Jeong Roh, Yu Jeong Song, Hee Jin Hong, Jin Tae Hwang, Dae Youn PLoS One Research Article CRISPR-Cas9-mediated leptin (Lep) knockout (KO) mice exhibited prominent phenotypes for constipation, even though they were not compared with other model animals. This study compared the stool excretion, gastrointestinal motility, histological structure, mucin secretion, and enteric nerve function in Lep KO and high fat diet (HFD)-treated mice to determine if there were differences in their phenotypes for constipation. Most obesity phenotypes, including fat weight, adipocyte size, expression of lipolytic proteins (HSL, perilipin, and ATGL), and glucose concentrations, were detected similarly in the Lep KO and HFD-treated mice. They showed a similar decrease in the excretion parameters, including the stool number, weight, and water content, while the same pattern was detected in the gastrointestinal motility and intestinal length. A similar decrease in the mucosal layer thickness, muscle thickness, ability for mucin secretion, and expression of water channel (aquaporin 3 and 8) genes was detected in the mid-colon of the Lep KO and HFD-treated mice, but the alteration rate in some levels was greater in the HFD-treated group than the Lep KO mice. On the other hand, the levels of c-kit, nNOS, NSE, and PGP9.5 expression for the enteric neurons and intestitial cells of Cajal (ICC) were remarkably lower in the mid-colon of the HFD-treated mice than in the Lep KO mice, but the level of most proteins in both groups remained lower than those in the control group. A similar alteration pattern in the expression of muscarinic acetylcholine receptors (mAChRs) and serotonin receptors was detected in the Lep KO and HFD-treated mice. These results suggest that most phenotypes for obesity-induced constipation were similarly detected in the Lep KO and HFD-treated mice, but there was a difference in the regulatory function of the enteric nervous system (ENS). Public Library of Science 2022-12-22 /pmc/articles/PMC9778951/ /pubmed/36548335 http://dx.doi.org/10.1371/journal.pone.0276445 Text en © 2022 Kim et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kim, Ji Eun
Seol, Ayun
Choi, Yun Ju
Lee, Su Jin
Jin, You Jeong
Roh, Yu Jeong
Song, Hee Jin
Hong, Jin Tae
Hwang, Dae Youn
Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice
title Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice
title_full Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice
title_fullStr Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice
title_full_unstemmed Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice
title_short Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice
title_sort similarities and differences in constipation phenotypes between lep knockout mice and high fat diet-induced obesity mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9778951/
https://www.ncbi.nlm.nih.gov/pubmed/36548335
http://dx.doi.org/10.1371/journal.pone.0276445
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