Cargando…

New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment

Cancers are defined by genetic defects, which underlines the prospect of using gene therapy in patient care. During the past decade, CRISPR technology has rapidly evolved into a powerful gene editing tool with high fidelity and precision. However, one of the impediments slowing down the clinical tra...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Biying, Liang, Yaxuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779094/
https://www.ncbi.nlm.nih.gov/pubmed/36555398
http://dx.doi.org/10.3390/ijms232415758
_version_ 1784856525245775872
author Yan, Biying
Liang, Yaxuan
author_facet Yan, Biying
Liang, Yaxuan
author_sort Yan, Biying
collection PubMed
description Cancers are defined by genetic defects, which underlines the prospect of using gene therapy in patient care. During the past decade, CRISPR technology has rapidly evolved into a powerful gene editing tool with high fidelity and precision. However, one of the impediments slowing down the clinical translation of CRISPR-based gene therapy concerns the lack of ideal delivery vectors. Extracellular vesicles (EVs) are nano-sized membrane sacs naturally released from nearly all types of cells. Although EVs are secreted for bio-information conveyance among cells or tissues, they have been recognized as superior vectors for drug or gene delivery. Recently, emerging evidence has spotlighted EVs in CRISPR delivery towards cancer treatment. In this review, we briefly introduce the biology and function of the CRISPR system and follow this with a summary of current delivery methods for CRISPR applications. We emphasize the recent progress in EV-mediated CRISPR editing for various cancer types and target genes. The reported strategies for constructing EV-CRISPR vectors, as well as their limitations, are discussed in detail. The review aims to throw light on the clinical potential of engineered EVs and encourage the expansion of our available toolkit to defeat cancer.
format Online
Article
Text
id pubmed-9779094
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97790942022-12-23 New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment Yan, Biying Liang, Yaxuan Int J Mol Sci Review Cancers are defined by genetic defects, which underlines the prospect of using gene therapy in patient care. During the past decade, CRISPR technology has rapidly evolved into a powerful gene editing tool with high fidelity and precision. However, one of the impediments slowing down the clinical translation of CRISPR-based gene therapy concerns the lack of ideal delivery vectors. Extracellular vesicles (EVs) are nano-sized membrane sacs naturally released from nearly all types of cells. Although EVs are secreted for bio-information conveyance among cells or tissues, they have been recognized as superior vectors for drug or gene delivery. Recently, emerging evidence has spotlighted EVs in CRISPR delivery towards cancer treatment. In this review, we briefly introduce the biology and function of the CRISPR system and follow this with a summary of current delivery methods for CRISPR applications. We emphasize the recent progress in EV-mediated CRISPR editing for various cancer types and target genes. The reported strategies for constructing EV-CRISPR vectors, as well as their limitations, are discussed in detail. The review aims to throw light on the clinical potential of engineered EVs and encourage the expansion of our available toolkit to defeat cancer. MDPI 2022-12-12 /pmc/articles/PMC9779094/ /pubmed/36555398 http://dx.doi.org/10.3390/ijms232415758 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Yan, Biying
Liang, Yaxuan
New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment
title New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment
title_full New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment
title_fullStr New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment
title_full_unstemmed New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment
title_short New Therapeutics for Extracellular Vesicles: Delivering CRISPR for Cancer Treatment
title_sort new therapeutics for extracellular vesicles: delivering crispr for cancer treatment
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779094/
https://www.ncbi.nlm.nih.gov/pubmed/36555398
http://dx.doi.org/10.3390/ijms232415758
work_keys_str_mv AT yanbiying newtherapeuticsforextracellularvesiclesdeliveringcrisprforcancertreatment
AT liangyaxuan newtherapeuticsforextracellularvesiclesdeliveringcrisprforcancertreatment