Cargando…
Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius)
Soybean is frequently attacked by herbivorous pests throughout the growth period. Exploring anti-insect genes to improve insect resistance in soybean is an important soybean breeding goal. Here, we cloned and characterized the gene for a quantitative trait locus (QTL) related to insect resistance, G...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779107/ https://www.ncbi.nlm.nih.gov/pubmed/36555336 http://dx.doi.org/10.3390/ijms232415696 |
_version_ | 1784856528594927616 |
---|---|
author | Wang, Huiqi Li, Xiao Su, Fenglin Liu, Hailun Hu, Dezhou Huang, Fang Yu, Deyue Wang, Hui |
author_facet | Wang, Huiqi Li, Xiao Su, Fenglin Liu, Hailun Hu, Dezhou Huang, Fang Yu, Deyue Wang, Hui |
author_sort | Wang, Huiqi |
collection | PubMed |
description | Soybean is frequently attacked by herbivorous pests throughout the growth period. Exploring anti-insect genes to improve insect resistance in soybean is an important soybean breeding goal. Here, we cloned and characterized the gene for a quantitative trait locus (QTL) related to insect resistance, Glyma.06g189600, which encodes CALCIUM-DEPENDENT PROTEIN KINASE17 (GmCDPK17) in soybean. The pairwise sequence alignment analysis revealed that the presumed protein of GmCDPK17 shares 52.06% similarity with that of GmCDPK38, a known negative regulatory gene of insect resistance in soybean. Ectopic expression of GmCDPK17 and GmCDPK38 restored the phenotypes of the Arabidopsis insect-susceptible mutant cpk10 and insect-resistant mutant cpk28, respectively. Moreover, transgenic hairy roots of the soybean cultivar Jack were generated by Agrobacterium-mediated transformation. Overexpression of GmCDPK17 increased soybean hairy root resistance to common cutworm (CCW), while RNA interference of the gene decreased soybean hairy root resistance to CCW. Sequencing data from the cultivated and wild soybeans were used to analyze the genetic diversity of GmCDPK17. This gene was subjected to domestication selection. Six and seven haplotypes (Haps) were identified in cultivated and wild soybeans, respectively. The resistance Hap1 is not widely used in cultivated soybeans and is mainly distributed at low latitudes. Accessions with resistance haplotypes of the GmCDPK17 and GmCDPK38 genes showed high resistance to CCW. Altogether, we revealed a novel positive regulatory insect resistance gene, GmCDPK17, which may further improve insect resistance in soybean. |
format | Online Article Text |
id | pubmed-9779107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97791072022-12-23 Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) Wang, Huiqi Li, Xiao Su, Fenglin Liu, Hailun Hu, Dezhou Huang, Fang Yu, Deyue Wang, Hui Int J Mol Sci Article Soybean is frequently attacked by herbivorous pests throughout the growth period. Exploring anti-insect genes to improve insect resistance in soybean is an important soybean breeding goal. Here, we cloned and characterized the gene for a quantitative trait locus (QTL) related to insect resistance, Glyma.06g189600, which encodes CALCIUM-DEPENDENT PROTEIN KINASE17 (GmCDPK17) in soybean. The pairwise sequence alignment analysis revealed that the presumed protein of GmCDPK17 shares 52.06% similarity with that of GmCDPK38, a known negative regulatory gene of insect resistance in soybean. Ectopic expression of GmCDPK17 and GmCDPK38 restored the phenotypes of the Arabidopsis insect-susceptible mutant cpk10 and insect-resistant mutant cpk28, respectively. Moreover, transgenic hairy roots of the soybean cultivar Jack were generated by Agrobacterium-mediated transformation. Overexpression of GmCDPK17 increased soybean hairy root resistance to common cutworm (CCW), while RNA interference of the gene decreased soybean hairy root resistance to CCW. Sequencing data from the cultivated and wild soybeans were used to analyze the genetic diversity of GmCDPK17. This gene was subjected to domestication selection. Six and seven haplotypes (Haps) were identified in cultivated and wild soybeans, respectively. The resistance Hap1 is not widely used in cultivated soybeans and is mainly distributed at low latitudes. Accessions with resistance haplotypes of the GmCDPK17 and GmCDPK38 genes showed high resistance to CCW. Altogether, we revealed a novel positive regulatory insect resistance gene, GmCDPK17, which may further improve insect resistance in soybean. MDPI 2022-12-10 /pmc/articles/PMC9779107/ /pubmed/36555336 http://dx.doi.org/10.3390/ijms232415696 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Huiqi Li, Xiao Su, Fenglin Liu, Hailun Hu, Dezhou Huang, Fang Yu, Deyue Wang, Hui Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) |
title | Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) |
title_full | Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) |
title_fullStr | Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) |
title_full_unstemmed | Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) |
title_short | Soybean CALCIUM-DEPENDENT PROTEIN KINASE17 Positively Regulates Plant Resistance to Common Cutworm (Spodoptera litura Fabricius) |
title_sort | soybean calcium-dependent protein kinase17 positively regulates plant resistance to common cutworm (spodoptera litura fabricius) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779107/ https://www.ncbi.nlm.nih.gov/pubmed/36555336 http://dx.doi.org/10.3390/ijms232415696 |
work_keys_str_mv | AT wanghuiqi soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT lixiao soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT sufenglin soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT liuhailun soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT hudezhou soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT huangfang soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT yudeyue soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius AT wanghui soybeancalciumdependentproteinkinase17positivelyregulatesplantresistancetocommoncutwormspodopteraliturafabricius |