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Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database

Extracellular matrix (ECM) changes after myocardial infarction (MI) need precise regulation, and next-generation sequencing technologies provide omics data that can be used in this context. We performed a meta-analysis using RNA-sequencing transcriptomic datasets to identify genes involved in post-M...

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Autores principales: Ortega, María, Ríos-Navarro, César, Gavara, Jose, de Dios, Elena, Perez-Solé, Nerea, Marcos-Garcés, Victor, Ferrández-Izquierdo, Antonio, Bodí, Vicente, Ruiz-Saurí, Amparo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779146/
https://www.ncbi.nlm.nih.gov/pubmed/36555255
http://dx.doi.org/10.3390/ijms232415615
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author Ortega, María
Ríos-Navarro, César
Gavara, Jose
de Dios, Elena
Perez-Solé, Nerea
Marcos-Garcés, Victor
Ferrández-Izquierdo, Antonio
Bodí, Vicente
Ruiz-Saurí, Amparo
author_facet Ortega, María
Ríos-Navarro, César
Gavara, Jose
de Dios, Elena
Perez-Solé, Nerea
Marcos-Garcés, Victor
Ferrández-Izquierdo, Antonio
Bodí, Vicente
Ruiz-Saurí, Amparo
author_sort Ortega, María
collection PubMed
description Extracellular matrix (ECM) changes after myocardial infarction (MI) need precise regulation, and next-generation sequencing technologies provide omics data that can be used in this context. We performed a meta-analysis using RNA-sequencing transcriptomic datasets to identify genes involved in post-MI ECM turnover. Eight studies available in Gene Expression Omnibus were selected following the inclusion criteria. We compare RNA-sequencing data from 92 mice submitted to permanent coronary ligation or sham, identifying differentially expressed genes (p-value < 0.05 and Log2FoldChange ≥ 2). Functional enrichment analysis was performed based on Gene Ontology biological processes (BPs). BPs implicated in response to extracellular stimulus, regulation of ECM organization, and ECM disassembly were detected soon after ischemia onset. ECM disassembly occurred between days one to seven post-MI, compared with ECM assembly from day seven onwards. We identified altered mRNA expression of 19 matrix metalloproteinases and four tissue inhibitors of metalloproteinases at post-infarcted ECM remodeling and altered transcriptomic expression of 42 genes encoding 26 collagen subunits at the fibrotic stage. To our knowledge, this is the first meta-analysis using RNA-sequencing datasets to evaluate post-infarcted cardiac interstitium healing, revealing previously unknown mechanisms and molecules actively implicated in ECM remodeling post-MI, which warrant further validation.
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spelling pubmed-97791462022-12-23 Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database Ortega, María Ríos-Navarro, César Gavara, Jose de Dios, Elena Perez-Solé, Nerea Marcos-Garcés, Victor Ferrández-Izquierdo, Antonio Bodí, Vicente Ruiz-Saurí, Amparo Int J Mol Sci Article Extracellular matrix (ECM) changes after myocardial infarction (MI) need precise regulation, and next-generation sequencing technologies provide omics data that can be used in this context. We performed a meta-analysis using RNA-sequencing transcriptomic datasets to identify genes involved in post-MI ECM turnover. Eight studies available in Gene Expression Omnibus were selected following the inclusion criteria. We compare RNA-sequencing data from 92 mice submitted to permanent coronary ligation or sham, identifying differentially expressed genes (p-value < 0.05 and Log2FoldChange ≥ 2). Functional enrichment analysis was performed based on Gene Ontology biological processes (BPs). BPs implicated in response to extracellular stimulus, regulation of ECM organization, and ECM disassembly were detected soon after ischemia onset. ECM disassembly occurred between days one to seven post-MI, compared with ECM assembly from day seven onwards. We identified altered mRNA expression of 19 matrix metalloproteinases and four tissue inhibitors of metalloproteinases at post-infarcted ECM remodeling and altered transcriptomic expression of 42 genes encoding 26 collagen subunits at the fibrotic stage. To our knowledge, this is the first meta-analysis using RNA-sequencing datasets to evaluate post-infarcted cardiac interstitium healing, revealing previously unknown mechanisms and molecules actively implicated in ECM remodeling post-MI, which warrant further validation. MDPI 2022-12-09 /pmc/articles/PMC9779146/ /pubmed/36555255 http://dx.doi.org/10.3390/ijms232415615 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ortega, María
Ríos-Navarro, César
Gavara, Jose
de Dios, Elena
Perez-Solé, Nerea
Marcos-Garcés, Victor
Ferrández-Izquierdo, Antonio
Bodí, Vicente
Ruiz-Saurí, Amparo
Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database
title Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database
title_full Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database
title_fullStr Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database
title_full_unstemmed Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database
title_short Meta-Analysis of Extracellular Matrix Dynamics after Myocardial Infarction Using RNA-Sequencing Transcriptomic Database
title_sort meta-analysis of extracellular matrix dynamics after myocardial infarction using rna-sequencing transcriptomic database
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779146/
https://www.ncbi.nlm.nih.gov/pubmed/36555255
http://dx.doi.org/10.3390/ijms232415615
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