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Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells

Polyvinyl alcohol (PVA) hydrogels are well-known biomimetic 3D systems for mammalian cell cultures to mimic native tissues. Recently, several biomolecules were intended for use in PVA hydrogels to improve their biological properties. However, retinol, an important biomolecule, has not been combined...

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Autores principales: Elango, Jeevithan, Zamora-Ledezma, Camilo, Negrete-Bolagay, Daniela, Aza, Piedad N. De, Gómez-López, Vicente M., López-González, Ivan, Belén Hernández, Ana, De Val, José Eduardo Maté Sánchez, Wu, Wenhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779207/
https://www.ncbi.nlm.nih.gov/pubmed/36555266
http://dx.doi.org/10.3390/ijms232415623
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author Elango, Jeevithan
Zamora-Ledezma, Camilo
Negrete-Bolagay, Daniela
Aza, Piedad N. De
Gómez-López, Vicente M.
López-González, Ivan
Belén Hernández, Ana
De Val, José Eduardo Maté Sánchez
Wu, Wenhui
author_facet Elango, Jeevithan
Zamora-Ledezma, Camilo
Negrete-Bolagay, Daniela
Aza, Piedad N. De
Gómez-López, Vicente M.
López-González, Ivan
Belén Hernández, Ana
De Val, José Eduardo Maté Sánchez
Wu, Wenhui
author_sort Elango, Jeevithan
collection PubMed
description Polyvinyl alcohol (PVA) hydrogels are well-known biomimetic 3D systems for mammalian cell cultures to mimic native tissues. Recently, several biomolecules were intended for use in PVA hydrogels to improve their biological properties. However, retinol, an important biomolecule, has not been combined with a PVA hydrogel for culturing bone marrow mesenchymal stem (BMMS) cells. Thus, for the first time, the effect of retinol on the physicochemical, antimicrobial, and cell proliferative properties of a PVA hydrogel was investigated. The ability of protein (3.15 nm) and mineral adsorption (4.8 mg/mL) of a PVA hydrogel was improved by 0.5 wt.% retinol. The antimicrobial effect of hydrogel was more significant in S. aureus (39.3 mm) than in E. coli (14.6 mm), and the effect was improved by increasing the retinol concentration. The BMMS cell proliferation was more upregulated in retinol-loaded PVA hydrogel than in the control at 7 days. We demonstrate that the respective in vitro degradation rate of retinol-loaded PVA hydrogels (RPH) (75–78% degradation) may promote both antibacterial and cellular proliferation. Interestingly, the incorporation of retinol did not affect the cell-loading capacity of PVA hydrogel. Accordingly, the fabricated PVA retinol hydrogel proved its compatibility in a stem cell culture and could be a potential biomaterial for tissue regeneration.
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spelling pubmed-97792072022-12-23 Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells Elango, Jeevithan Zamora-Ledezma, Camilo Negrete-Bolagay, Daniela Aza, Piedad N. De Gómez-López, Vicente M. López-González, Ivan Belén Hernández, Ana De Val, José Eduardo Maté Sánchez Wu, Wenhui Int J Mol Sci Article Polyvinyl alcohol (PVA) hydrogels are well-known biomimetic 3D systems for mammalian cell cultures to mimic native tissues. Recently, several biomolecules were intended for use in PVA hydrogels to improve their biological properties. However, retinol, an important biomolecule, has not been combined with a PVA hydrogel for culturing bone marrow mesenchymal stem (BMMS) cells. Thus, for the first time, the effect of retinol on the physicochemical, antimicrobial, and cell proliferative properties of a PVA hydrogel was investigated. The ability of protein (3.15 nm) and mineral adsorption (4.8 mg/mL) of a PVA hydrogel was improved by 0.5 wt.% retinol. The antimicrobial effect of hydrogel was more significant in S. aureus (39.3 mm) than in E. coli (14.6 mm), and the effect was improved by increasing the retinol concentration. The BMMS cell proliferation was more upregulated in retinol-loaded PVA hydrogel than in the control at 7 days. We demonstrate that the respective in vitro degradation rate of retinol-loaded PVA hydrogels (RPH) (75–78% degradation) may promote both antibacterial and cellular proliferation. Interestingly, the incorporation of retinol did not affect the cell-loading capacity of PVA hydrogel. Accordingly, the fabricated PVA retinol hydrogel proved its compatibility in a stem cell culture and could be a potential biomaterial for tissue regeneration. MDPI 2022-12-09 /pmc/articles/PMC9779207/ /pubmed/36555266 http://dx.doi.org/10.3390/ijms232415623 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elango, Jeevithan
Zamora-Ledezma, Camilo
Negrete-Bolagay, Daniela
Aza, Piedad N. De
Gómez-López, Vicente M.
López-González, Ivan
Belén Hernández, Ana
De Val, José Eduardo Maté Sánchez
Wu, Wenhui
Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells
title Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells
title_full Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells
title_fullStr Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells
title_full_unstemmed Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells
title_short Retinol-Loaded Poly(vinyl alcohol)-Based Hydrogels as Suitable Biomaterials with Antimicrobial Properties for the Proliferation of Mesenchymal Stem Cells
title_sort retinol-loaded poly(vinyl alcohol)-based hydrogels as suitable biomaterials with antimicrobial properties for the proliferation of mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779207/
https://www.ncbi.nlm.nih.gov/pubmed/36555266
http://dx.doi.org/10.3390/ijms232415623
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