Cargando…
Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair?
The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 7...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779304/ https://www.ncbi.nlm.nih.gov/pubmed/36555260 http://dx.doi.org/10.3390/ijms232415611 |
_version_ | 1784856576700448768 |
---|---|
author | Sardo, Ariane Venzon Naia Andrade, Maíra Franco Figueiredo, Anaeliza Rosin, Flávia Cristina Perillo Corrêa, Luciana Zezell, Denise Maria |
author_facet | Sardo, Ariane Venzon Naia Andrade, Maíra Franco Figueiredo, Anaeliza Rosin, Flávia Cristina Perillo Corrêa, Luciana Zezell, Denise Maria |
author_sort | Sardo, Ariane Venzon Naia |
collection | PubMed |
description | The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of rats, eight and twenty days after irradiation with single dose of 20 Gy. The percentage area corresponding to positive staining for cytokeratin 10 (CK10) and 14 (CK14) proteins was evaluated in the epithelial area of the lesions, using an immunohistochemical technique (IHC), 8 and 20 days after the induction of lesions, and compared with an untreated control group. CK10 was significantly more expressed in the group treated with 660 nm PBM. CK14 did not show quantitative differences between the groups evaluated. However, whereas in the groups treated with PBM, CK14 was already restricted to the basal layer of the epithelium, as expected in healthy epithelia, in control group it was also expressed in upper layers of the epithelium. In this work, PBM was able to improve epithelial maturity of the repaired OM wound, especially in the 660 nm group. |
format | Online Article Text |
id | pubmed-9779304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97793042022-12-23 Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? Sardo, Ariane Venzon Naia Andrade, Maíra Franco Figueiredo, Anaeliza Rosin, Flávia Cristina Perillo Corrêa, Luciana Zezell, Denise Maria Int J Mol Sci Communication The mechanisms of action of photobiomodulation (PBM) in oral mucositis (OM) are not completely elucidated. To enlighten the role of PBM in the evolution of epithelial maturity in OM ulcers, the present study evaluated the effect of PBM with red (λ) wavelength of 660 nanometers (nm) and infrared of 780 nm in radio-induced OM wounds on the tongue of rats, eight and twenty days after irradiation with single dose of 20 Gy. The percentage area corresponding to positive staining for cytokeratin 10 (CK10) and 14 (CK14) proteins was evaluated in the epithelial area of the lesions, using an immunohistochemical technique (IHC), 8 and 20 days after the induction of lesions, and compared with an untreated control group. CK10 was significantly more expressed in the group treated with 660 nm PBM. CK14 did not show quantitative differences between the groups evaluated. However, whereas in the groups treated with PBM, CK14 was already restricted to the basal layer of the epithelium, as expected in healthy epithelia, in control group it was also expressed in upper layers of the epithelium. In this work, PBM was able to improve epithelial maturity of the repaired OM wound, especially in the 660 nm group. MDPI 2022-12-09 /pmc/articles/PMC9779304/ /pubmed/36555260 http://dx.doi.org/10.3390/ijms232415611 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Sardo, Ariane Venzon Naia Andrade, Maíra Franco Figueiredo, Anaeliza Rosin, Flávia Cristina Perillo Corrêa, Luciana Zezell, Denise Maria Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? |
title | Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? |
title_full | Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? |
title_fullStr | Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? |
title_full_unstemmed | Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? |
title_short | Does Photobiomodulation Affects CK10 and CK14 in Oral Mucositis Radioinduced Repair? |
title_sort | does photobiomodulation affects ck10 and ck14 in oral mucositis radioinduced repair? |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779304/ https://www.ncbi.nlm.nih.gov/pubmed/36555260 http://dx.doi.org/10.3390/ijms232415611 |
work_keys_str_mv | AT sardoarianevenzonnaia doesphotobiomodulationaffectsck10andck14inoralmucositisradioinducedrepair AT andrademairafranco doesphotobiomodulationaffectsck10andck14inoralmucositisradioinducedrepair AT figueiredoanaeliza doesphotobiomodulationaffectsck10andck14inoralmucositisradioinducedrepair AT rosinflaviacristinaperillo doesphotobiomodulationaffectsck10andck14inoralmucositisradioinducedrepair AT correaluciana doesphotobiomodulationaffectsck10andck14inoralmucositisradioinducedrepair AT zezelldenisemaria doesphotobiomodulationaffectsck10andck14inoralmucositisradioinducedrepair |