Slight Variations in the Sequence Downstream of the Polyadenylation Signal Significantly Increase Transgene Expression in HEK293T and CHO Cells

Compared to transcription initiation, much less is known about transcription termination. In particular, large-scale mutagenesis studies have, so far, primarily concentrated on promoter and enhancer, but not terminator sequences. Here, we used a massively parallel reporter assay (MPRA) to systematic...

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Detalles Bibliográficos
Autores principales: Omelina, Evgeniya S., Letiagina, Anna E., Boldyreva, Lidiya V., Ogienko, Anna A., Galimova, Yuliya A., Yarinich, Lyubov A., Pindyurin, Alexey V., Andreyeva, Evgeniya N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779314/
https://www.ncbi.nlm.nih.gov/pubmed/36555130
http://dx.doi.org/10.3390/ijms232415485

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