Cargando…

Host Cell Binding Mediated by Leptospira interrogans Adhesins

Leptospirosis is a neglected infectious disease with global impact on both humans and animals. The increase in urban development without sanitation planning is one of the main reasons for the disease spreading. The symptoms are similar to those of flu-like diseases, such as dengue, yellow fever, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Takahashi, Maria Beatriz, Teixeira, Aline Florencio, Nascimento, Ana Lucia Tabet Oller
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779477/
https://www.ncbi.nlm.nih.gov/pubmed/36555188
http://dx.doi.org/10.3390/ijms232415550
_version_ 1784856619078647808
author Takahashi, Maria Beatriz
Teixeira, Aline Florencio
Nascimento, Ana Lucia Tabet Oller
author_facet Takahashi, Maria Beatriz
Teixeira, Aline Florencio
Nascimento, Ana Lucia Tabet Oller
author_sort Takahashi, Maria Beatriz
collection PubMed
description Leptospirosis is a neglected infectious disease with global impact on both humans and animals. The increase in urban development without sanitation planning is one of the main reasons for the disease spreading. The symptoms are similar to those of flu-like diseases, such as dengue, yellow fever, and malaria, which can result in a misleading clinical diagnosis. The characterization of host–pathogen interactions is important in the development of new vaccines, treatments, and diagnostics. However, the pathogenesis of leptospirosis is not well understood, and many gaps remain to be addressed. Here, we aimed to determine if Leptospira strains, virulent, culture-attenuated, and saprophytic, and the major outer membrane proteins OmpL37, OmpL1, LipL21, LipL41, and LipL46 are able to adhere to different endothelial, epithelial and fibroblast cell lines in vitro. We showed that virulent leptospires robustly bind to all cells compared to the culture-attenuated and saprophytic lines. The recombinant proteins exhibited certain adhesion, but only OmpL1 and LipL41 were able to bind to several cell lines, either in monolayer or in cell suspension. Blocking OmpL1 with polyclonal antibodies caused a decrease in bacterial binding to cells, contrasting with an increase observed when anti-LipL41 antibodies were used. The adhesion of OmpL1 to HMEC-1 and EA.hy926 was inhibited when cells were pre-incubated with collagen IV, suggesting that both compete for the same cell receptor. We present here for the first time the interaction of five leptospiral outer membrane proteins with several cell lines, and we conclude that LipL41 and OmpL1 may have an impact on leptospiral adhesion to mammalian cells and may mediate the colonization process in leptospiral pathogenesis.
format Online
Article
Text
id pubmed-9779477
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97794772022-12-23 Host Cell Binding Mediated by Leptospira interrogans Adhesins Takahashi, Maria Beatriz Teixeira, Aline Florencio Nascimento, Ana Lucia Tabet Oller Int J Mol Sci Article Leptospirosis is a neglected infectious disease with global impact on both humans and animals. The increase in urban development without sanitation planning is one of the main reasons for the disease spreading. The symptoms are similar to those of flu-like diseases, such as dengue, yellow fever, and malaria, which can result in a misleading clinical diagnosis. The characterization of host–pathogen interactions is important in the development of new vaccines, treatments, and diagnostics. However, the pathogenesis of leptospirosis is not well understood, and many gaps remain to be addressed. Here, we aimed to determine if Leptospira strains, virulent, culture-attenuated, and saprophytic, and the major outer membrane proteins OmpL37, OmpL1, LipL21, LipL41, and LipL46 are able to adhere to different endothelial, epithelial and fibroblast cell lines in vitro. We showed that virulent leptospires robustly bind to all cells compared to the culture-attenuated and saprophytic lines. The recombinant proteins exhibited certain adhesion, but only OmpL1 and LipL41 were able to bind to several cell lines, either in monolayer or in cell suspension. Blocking OmpL1 with polyclonal antibodies caused a decrease in bacterial binding to cells, contrasting with an increase observed when anti-LipL41 antibodies were used. The adhesion of OmpL1 to HMEC-1 and EA.hy926 was inhibited when cells were pre-incubated with collagen IV, suggesting that both compete for the same cell receptor. We present here for the first time the interaction of five leptospiral outer membrane proteins with several cell lines, and we conclude that LipL41 and OmpL1 may have an impact on leptospiral adhesion to mammalian cells and may mediate the colonization process in leptospiral pathogenesis. MDPI 2022-12-08 /pmc/articles/PMC9779477/ /pubmed/36555188 http://dx.doi.org/10.3390/ijms232415550 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Takahashi, Maria Beatriz
Teixeira, Aline Florencio
Nascimento, Ana Lucia Tabet Oller
Host Cell Binding Mediated by Leptospira interrogans Adhesins
title Host Cell Binding Mediated by Leptospira interrogans Adhesins
title_full Host Cell Binding Mediated by Leptospira interrogans Adhesins
title_fullStr Host Cell Binding Mediated by Leptospira interrogans Adhesins
title_full_unstemmed Host Cell Binding Mediated by Leptospira interrogans Adhesins
title_short Host Cell Binding Mediated by Leptospira interrogans Adhesins
title_sort host cell binding mediated by leptospira interrogans adhesins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779477/
https://www.ncbi.nlm.nih.gov/pubmed/36555188
http://dx.doi.org/10.3390/ijms232415550
work_keys_str_mv AT takahashimariabeatriz hostcellbindingmediatedbyleptospirainterrogansadhesins
AT teixeiraalineflorencio hostcellbindingmediatedbyleptospirainterrogansadhesins
AT nascimentoanaluciatabetoller hostcellbindingmediatedbyleptospirainterrogansadhesins