Cargando…

Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis

The purpose of this study was to provide an immuno-mediated substantiation of the etiopathogenesis of mucositis and peri-implantitis based on the results of experimental, laboratory and clinical studies. The biopsy material was studied to identify impregnated nanoscale and microscale particles in th...

Descripción completa

Detalles Bibliográficos
Autores principales: Labis, Varvara, Bazikyan, Ernest, Sizova, Svetlana, Oleinikov, Vladimir, Trulioff, Andrey, Serebriakova, Maria, Kudryavtsev, Igor, Zhigalina, Olga, Khmelenin, Dmitry, Dyachkova, Irina, Zolotov, Denis, Asadchikov, Victor, Volkov, Alexey, Khaidukov, Sergey, Kozlov, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779511/
https://www.ncbi.nlm.nih.gov/pubmed/36555457
http://dx.doi.org/10.3390/ijms232415797
_version_ 1784856627547996160
author Labis, Varvara
Bazikyan, Ernest
Sizova, Svetlana
Oleinikov, Vladimir
Trulioff, Andrey
Serebriakova, Maria
Kudryavtsev, Igor
Zhigalina, Olga
Khmelenin, Dmitry
Dyachkova, Irina
Zolotov, Denis
Asadchikov, Victor
Volkov, Alexey
Khaidukov, Sergey
Kozlov, Ivan
author_facet Labis, Varvara
Bazikyan, Ernest
Sizova, Svetlana
Oleinikov, Vladimir
Trulioff, Andrey
Serebriakova, Maria
Kudryavtsev, Igor
Zhigalina, Olga
Khmelenin, Dmitry
Dyachkova, Irina
Zolotov, Denis
Asadchikov, Victor
Volkov, Alexey
Khaidukov, Sergey
Kozlov, Ivan
author_sort Labis, Varvara
collection PubMed
description The purpose of this study was to provide an immuno-mediated substantiation of the etiopathogenesis of mucositis and peri-implantitis based on the results of experimental, laboratory and clinical studies. The biopsy material was studied to identify impregnated nanoscale and microscale particles in the structure of pathological tissues by using X-ray microtomography and X-ray fluorescence analyses. Electron microscopy with energy-dispersive analysis identified the composition of supernatants containing nanoscale metal particles obtained from the surfaces of dental implants. The parameters of the nanoscale particles were determined by dynamic light scattering. Flow cytometry was used to study the effect of nanoscale particles on the ability to induce the activation and apoptosis of immunocompetent cells depending on the particles’ concentrations during cultivation with the monocytic cell line THP-1 with the addition of inductors. An analysis of the laboratory results suggested the presence of dose-dependent activation, as well as early and late apoptosis of the immunocompetent cells. Activation and early and late apoptosis of a monocytic cell line when THP-1 was co-cultured with nanoscale metal particles in supernatants were shown for the first time. When human venous blood plasma was added, both activation and early and late apoptosis had a dose-dependent effect and differed from those of the control groups.
format Online
Article
Text
id pubmed-9779511
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97795112022-12-23 Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis Labis, Varvara Bazikyan, Ernest Sizova, Svetlana Oleinikov, Vladimir Trulioff, Andrey Serebriakova, Maria Kudryavtsev, Igor Zhigalina, Olga Khmelenin, Dmitry Dyachkova, Irina Zolotov, Denis Asadchikov, Victor Volkov, Alexey Khaidukov, Sergey Kozlov, Ivan Int J Mol Sci Article The purpose of this study was to provide an immuno-mediated substantiation of the etiopathogenesis of mucositis and peri-implantitis based on the results of experimental, laboratory and clinical studies. The biopsy material was studied to identify impregnated nanoscale and microscale particles in the structure of pathological tissues by using X-ray microtomography and X-ray fluorescence analyses. Electron microscopy with energy-dispersive analysis identified the composition of supernatants containing nanoscale metal particles obtained from the surfaces of dental implants. The parameters of the nanoscale particles were determined by dynamic light scattering. Flow cytometry was used to study the effect of nanoscale particles on the ability to induce the activation and apoptosis of immunocompetent cells depending on the particles’ concentrations during cultivation with the monocytic cell line THP-1 with the addition of inductors. An analysis of the laboratory results suggested the presence of dose-dependent activation, as well as early and late apoptosis of the immunocompetent cells. Activation and early and late apoptosis of a monocytic cell line when THP-1 was co-cultured with nanoscale metal particles in supernatants were shown for the first time. When human venous blood plasma was added, both activation and early and late apoptosis had a dose-dependent effect and differed from those of the control groups. MDPI 2022-12-13 /pmc/articles/PMC9779511/ /pubmed/36555457 http://dx.doi.org/10.3390/ijms232415797 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Labis, Varvara
Bazikyan, Ernest
Sizova, Svetlana
Oleinikov, Vladimir
Trulioff, Andrey
Serebriakova, Maria
Kudryavtsev, Igor
Zhigalina, Olga
Khmelenin, Dmitry
Dyachkova, Irina
Zolotov, Denis
Asadchikov, Victor
Volkov, Alexey
Khaidukov, Sergey
Kozlov, Ivan
Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
title Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
title_full Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
title_fullStr Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
title_full_unstemmed Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
title_short Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
title_sort immunopathological inflammation in the evolution of mucositis and peri-implantitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779511/
https://www.ncbi.nlm.nih.gov/pubmed/36555457
http://dx.doi.org/10.3390/ijms232415797
work_keys_str_mv AT labisvarvara immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT bazikyanernest immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT sizovasvetlana immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT oleinikovvladimir immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT trulioffandrey immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT serebriakovamaria immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT kudryavtsevigor immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT zhigalinaolga immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT khmelenindmitry immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT dyachkovairina immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT zolotovdenis immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT asadchikovvictor immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT volkovalexey immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT khaidukovsergey immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis
AT kozlovivan immunopathologicalinflammationintheevolutionofmucositisandperiimplantitis