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Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis
The purpose of this study was to provide an immuno-mediated substantiation of the etiopathogenesis of mucositis and peri-implantitis based on the results of experimental, laboratory and clinical studies. The biopsy material was studied to identify impregnated nanoscale and microscale particles in th...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779511/ https://www.ncbi.nlm.nih.gov/pubmed/36555457 http://dx.doi.org/10.3390/ijms232415797 |
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author | Labis, Varvara Bazikyan, Ernest Sizova, Svetlana Oleinikov, Vladimir Trulioff, Andrey Serebriakova, Maria Kudryavtsev, Igor Zhigalina, Olga Khmelenin, Dmitry Dyachkova, Irina Zolotov, Denis Asadchikov, Victor Volkov, Alexey Khaidukov, Sergey Kozlov, Ivan |
author_facet | Labis, Varvara Bazikyan, Ernest Sizova, Svetlana Oleinikov, Vladimir Trulioff, Andrey Serebriakova, Maria Kudryavtsev, Igor Zhigalina, Olga Khmelenin, Dmitry Dyachkova, Irina Zolotov, Denis Asadchikov, Victor Volkov, Alexey Khaidukov, Sergey Kozlov, Ivan |
author_sort | Labis, Varvara |
collection | PubMed |
description | The purpose of this study was to provide an immuno-mediated substantiation of the etiopathogenesis of mucositis and peri-implantitis based on the results of experimental, laboratory and clinical studies. The biopsy material was studied to identify impregnated nanoscale and microscale particles in the structure of pathological tissues by using X-ray microtomography and X-ray fluorescence analyses. Electron microscopy with energy-dispersive analysis identified the composition of supernatants containing nanoscale metal particles obtained from the surfaces of dental implants. The parameters of the nanoscale particles were determined by dynamic light scattering. Flow cytometry was used to study the effect of nanoscale particles on the ability to induce the activation and apoptosis of immunocompetent cells depending on the particles’ concentrations during cultivation with the monocytic cell line THP-1 with the addition of inductors. An analysis of the laboratory results suggested the presence of dose-dependent activation, as well as early and late apoptosis of the immunocompetent cells. Activation and early and late apoptosis of a monocytic cell line when THP-1 was co-cultured with nanoscale metal particles in supernatants were shown for the first time. When human venous blood plasma was added, both activation and early and late apoptosis had a dose-dependent effect and differed from those of the control groups. |
format | Online Article Text |
id | pubmed-9779511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97795112022-12-23 Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis Labis, Varvara Bazikyan, Ernest Sizova, Svetlana Oleinikov, Vladimir Trulioff, Andrey Serebriakova, Maria Kudryavtsev, Igor Zhigalina, Olga Khmelenin, Dmitry Dyachkova, Irina Zolotov, Denis Asadchikov, Victor Volkov, Alexey Khaidukov, Sergey Kozlov, Ivan Int J Mol Sci Article The purpose of this study was to provide an immuno-mediated substantiation of the etiopathogenesis of mucositis and peri-implantitis based on the results of experimental, laboratory and clinical studies. The biopsy material was studied to identify impregnated nanoscale and microscale particles in the structure of pathological tissues by using X-ray microtomography and X-ray fluorescence analyses. Electron microscopy with energy-dispersive analysis identified the composition of supernatants containing nanoscale metal particles obtained from the surfaces of dental implants. The parameters of the nanoscale particles were determined by dynamic light scattering. Flow cytometry was used to study the effect of nanoscale particles on the ability to induce the activation and apoptosis of immunocompetent cells depending on the particles’ concentrations during cultivation with the monocytic cell line THP-1 with the addition of inductors. An analysis of the laboratory results suggested the presence of dose-dependent activation, as well as early and late apoptosis of the immunocompetent cells. Activation and early and late apoptosis of a monocytic cell line when THP-1 was co-cultured with nanoscale metal particles in supernatants were shown for the first time. When human venous blood plasma was added, both activation and early and late apoptosis had a dose-dependent effect and differed from those of the control groups. MDPI 2022-12-13 /pmc/articles/PMC9779511/ /pubmed/36555457 http://dx.doi.org/10.3390/ijms232415797 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Labis, Varvara Bazikyan, Ernest Sizova, Svetlana Oleinikov, Vladimir Trulioff, Andrey Serebriakova, Maria Kudryavtsev, Igor Zhigalina, Olga Khmelenin, Dmitry Dyachkova, Irina Zolotov, Denis Asadchikov, Victor Volkov, Alexey Khaidukov, Sergey Kozlov, Ivan Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis |
title | Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis |
title_full | Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis |
title_fullStr | Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis |
title_full_unstemmed | Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis |
title_short | Immunopathological Inflammation in the Evolution of Mucositis and Peri-Implantitis |
title_sort | immunopathological inflammation in the evolution of mucositis and peri-implantitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779511/ https://www.ncbi.nlm.nih.gov/pubmed/36555457 http://dx.doi.org/10.3390/ijms232415797 |
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