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Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions

Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The...

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Autores principales: Oberemok, Volodymyr V., Useinov, Refat Z., Skorokhod, Oleksii A., Gal’chinsky, Nikita V., Novikov, Ilya A., Makalish, Tatyana P., Yatskova, Ekaterina V., Sharmagiy, Alexander K., Golovkin, Ilya O., Gninenko, Yuri I., Puzanova, Yelizaveta V., Andreeva, Oksana A., Alieva, Edie E., Eken, Emre, Laikova, Kateryna V., Plugatar, Yuri V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779641/
https://www.ncbi.nlm.nih.gov/pubmed/36555325
http://dx.doi.org/10.3390/ijms232415681
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author Oberemok, Volodymyr V.
Useinov, Refat Z.
Skorokhod, Oleksii A.
Gal’chinsky, Nikita V.
Novikov, Ilya A.
Makalish, Tatyana P.
Yatskova, Ekaterina V.
Sharmagiy, Alexander K.
Golovkin, Ilya O.
Gninenko, Yuri I.
Puzanova, Yelizaveta V.
Andreeva, Oksana A.
Alieva, Edie E.
Eken, Emre
Laikova, Kateryna V.
Plugatar, Yuri V.
author_facet Oberemok, Volodymyr V.
Useinov, Refat Z.
Skorokhod, Oleksii A.
Gal’chinsky, Nikita V.
Novikov, Ilya A.
Makalish, Tatyana P.
Yatskova, Ekaterina V.
Sharmagiy, Alexander K.
Golovkin, Ilya O.
Gninenko, Yuri I.
Puzanova, Yelizaveta V.
Andreeva, Oksana A.
Alieva, Edie E.
Eken, Emre
Laikova, Kateryna V.
Plugatar, Yuri V.
author_sort Oberemok, Volodymyr V.
collection PubMed
description Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The current use of chemical insecticides leads to the accumulation of xenobiotics in ecosystems and a decreased number of species in those ecosystems, including insects. Sustainable development of human society is impossible without useful insects, so the control of insect pests must be effective and selective at the same time. In this article, we show for the first time a natural way to regulate the number of insect pests based on the use of extracellular double-stranded DNA secreted by the plant Pittosporum tobira. Using a principle similar to one found in nature, we show that the topical application of artificially synthesized short antisense oligonucleotide insecticides (olinscides, DNA insecticides) is an effective and selective way to control the insect Coccus hesperidum. Using contact oligonucleotide insecticide Coccus-11 at a concentration of 100 ng/μL on C. hesperidum larvae resulted in a mortality of 95.59 ± 1.63% within 12 days. Green oligonucleotide insecticides, created by nature and later discovered by humans, demonstrate a new method to control insect pests that is beneficial and safe for macromolecular insect pest management.
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spelling pubmed-97796412022-12-23 Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions Oberemok, Volodymyr V. Useinov, Refat Z. Skorokhod, Oleksii A. Gal’chinsky, Nikita V. Novikov, Ilya A. Makalish, Tatyana P. Yatskova, Ekaterina V. Sharmagiy, Alexander K. Golovkin, Ilya O. Gninenko, Yuri I. Puzanova, Yelizaveta V. Andreeva, Oksana A. Alieva, Edie E. Eken, Emre Laikova, Kateryna V. Plugatar, Yuri V. Int J Mol Sci Article Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The current use of chemical insecticides leads to the accumulation of xenobiotics in ecosystems and a decreased number of species in those ecosystems, including insects. Sustainable development of human society is impossible without useful insects, so the control of insect pests must be effective and selective at the same time. In this article, we show for the first time a natural way to regulate the number of insect pests based on the use of extracellular double-stranded DNA secreted by the plant Pittosporum tobira. Using a principle similar to one found in nature, we show that the topical application of artificially synthesized short antisense oligonucleotide insecticides (olinscides, DNA insecticides) is an effective and selective way to control the insect Coccus hesperidum. Using contact oligonucleotide insecticide Coccus-11 at a concentration of 100 ng/μL on C. hesperidum larvae resulted in a mortality of 95.59 ± 1.63% within 12 days. Green oligonucleotide insecticides, created by nature and later discovered by humans, demonstrate a new method to control insect pests that is beneficial and safe for macromolecular insect pest management. MDPI 2022-12-10 /pmc/articles/PMC9779641/ /pubmed/36555325 http://dx.doi.org/10.3390/ijms232415681 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oberemok, Volodymyr V.
Useinov, Refat Z.
Skorokhod, Oleksii A.
Gal’chinsky, Nikita V.
Novikov, Ilya A.
Makalish, Tatyana P.
Yatskova, Ekaterina V.
Sharmagiy, Alexander K.
Golovkin, Ilya O.
Gninenko, Yuri I.
Puzanova, Yelizaveta V.
Andreeva, Oksana A.
Alieva, Edie E.
Eken, Emre
Laikova, Kateryna V.
Plugatar, Yuri V.
Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
title Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
title_full Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
title_fullStr Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
title_full_unstemmed Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
title_short Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
title_sort oligonucleotide insecticides for green agriculture: regulatory role of contact dna in plant–insect interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779641/
https://www.ncbi.nlm.nih.gov/pubmed/36555325
http://dx.doi.org/10.3390/ijms232415681
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