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Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients

Here, we examined the dynamics of the gut and respiratory microbiomes in severe COVID-19 patients in need of mechanical ventilation in the intensive care unit (ICU). We recruited 85 critically ill patients (53 with COVID-19 and 32 without COVID-19) and 17 healthy controls (HCs) and monitored them fo...

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Autores principales: Cuenca, Silvia, Soler, Zaida, Serrano-Gómez, Gerard, Xie, Zixuan, Barquinero, Jordi, Roca, Joaquim, Sirvent, Jose-Maria, Manichanh, Chaysavanh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779860/
https://www.ncbi.nlm.nih.gov/pubmed/36555453
http://dx.doi.org/10.3390/ijms232415808
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author Cuenca, Silvia
Soler, Zaida
Serrano-Gómez, Gerard
Xie, Zixuan
Barquinero, Jordi
Roca, Joaquim
Sirvent, Jose-Maria
Manichanh, Chaysavanh
author_facet Cuenca, Silvia
Soler, Zaida
Serrano-Gómez, Gerard
Xie, Zixuan
Barquinero, Jordi
Roca, Joaquim
Sirvent, Jose-Maria
Manichanh, Chaysavanh
author_sort Cuenca, Silvia
collection PubMed
description Here, we examined the dynamics of the gut and respiratory microbiomes in severe COVID-19 patients in need of mechanical ventilation in the intensive care unit (ICU). We recruited 85 critically ill patients (53 with COVID-19 and 32 without COVID-19) and 17 healthy controls (HCs) and monitored them for up to 4 weeks. We analyzed the bacterial and fungal taxonomic profiles and loads of 232 gut and respiratory samples and we measured the blood levels of Interleukin 6, IgG, and IgM in COVID-19 patients. Upon ICU admission, the bacterial composition and load in the gut and respiratory samples were altered in critically ill patients compared with HCs. During their ICU stay, the patients experienced increased bacterial and fungal loads, drastic decreased bacterial richness, and progressive changes in bacterial and fungal taxonomic profiles. In the gut samples, six bacterial taxa could discriminate ICU-COV(+) from ICU-COV(−) cases upon ICU admission and the bacterial taxa were associated according to age, PaO2/FiO2, and CRP levels. In the respiratory samples of the ICU-COV(+) patients, bacterial signatures including Pseudomonas and Streptococcus were found to be correlated with the length of ICU stay. Our findings demonstrated that the gut and respiratory microbiome dysbiosis and bacterial signatures associated with critical illness emerged as biomarkers of COVID-19 severity and could be a potential predictor of ICU length of stay. We propose using a high-throughput sequencing approach as an alternative to traditional isolation techniques to monitor ICU patient infection.
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spelling pubmed-97798602022-12-23 Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients Cuenca, Silvia Soler, Zaida Serrano-Gómez, Gerard Xie, Zixuan Barquinero, Jordi Roca, Joaquim Sirvent, Jose-Maria Manichanh, Chaysavanh Int J Mol Sci Article Here, we examined the dynamics of the gut and respiratory microbiomes in severe COVID-19 patients in need of mechanical ventilation in the intensive care unit (ICU). We recruited 85 critically ill patients (53 with COVID-19 and 32 without COVID-19) and 17 healthy controls (HCs) and monitored them for up to 4 weeks. We analyzed the bacterial and fungal taxonomic profiles and loads of 232 gut and respiratory samples and we measured the blood levels of Interleukin 6, IgG, and IgM in COVID-19 patients. Upon ICU admission, the bacterial composition and load in the gut and respiratory samples were altered in critically ill patients compared with HCs. During their ICU stay, the patients experienced increased bacterial and fungal loads, drastic decreased bacterial richness, and progressive changes in bacterial and fungal taxonomic profiles. In the gut samples, six bacterial taxa could discriminate ICU-COV(+) from ICU-COV(−) cases upon ICU admission and the bacterial taxa were associated according to age, PaO2/FiO2, and CRP levels. In the respiratory samples of the ICU-COV(+) patients, bacterial signatures including Pseudomonas and Streptococcus were found to be correlated with the length of ICU stay. Our findings demonstrated that the gut and respiratory microbiome dysbiosis and bacterial signatures associated with critical illness emerged as biomarkers of COVID-19 severity and could be a potential predictor of ICU length of stay. We propose using a high-throughput sequencing approach as an alternative to traditional isolation techniques to monitor ICU patient infection. MDPI 2022-12-13 /pmc/articles/PMC9779860/ /pubmed/36555453 http://dx.doi.org/10.3390/ijms232415808 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cuenca, Silvia
Soler, Zaida
Serrano-Gómez, Gerard
Xie, Zixuan
Barquinero, Jordi
Roca, Joaquim
Sirvent, Jose-Maria
Manichanh, Chaysavanh
Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
title Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
title_full Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
title_fullStr Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
title_full_unstemmed Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
title_short Dysbiosis: An Indicator of COVID-19 Severity in Critically Ill Patients
title_sort dysbiosis: an indicator of covid-19 severity in critically ill patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779860/
https://www.ncbi.nlm.nih.gov/pubmed/36555453
http://dx.doi.org/10.3390/ijms232415808
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