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Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves

Plant species are known to harbor large number of endophytes, which stays in plant tissues as symbionts. These endophytes secrete large array of bioactive compounds that have potency against certain diseases with no side effects. We have collected leaf samples of the Moringa oleifera plant from the...

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Autores principales: Rehman, Bushra, Khan, Sumera Afzal, Hamayun, Muhammad, Iqbal, Amjad, Lee, In-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779999/
https://www.ncbi.nlm.nih.gov/pubmed/36567913
http://dx.doi.org/10.1155/2022/2461021
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author Rehman, Bushra
Khan, Sumera Afzal
Hamayun, Muhammad
Iqbal, Amjad
Lee, In-Jung
author_facet Rehman, Bushra
Khan, Sumera Afzal
Hamayun, Muhammad
Iqbal, Amjad
Lee, In-Jung
author_sort Rehman, Bushra
collection PubMed
description Plant species are known to harbor large number of endophytes, which stays in plant tissues as symbionts. These endophytes secrete large array of bioactive compounds that have potency against certain diseases with no side effects. We have collected leaf samples of the Moringa oleifera plant from the Pakistan Forest Institute, Khyber Pakhtunkhwa, Pakistan for the isolation of beneficial endophytes. The strains isolated from the leaves of M. oleifera were coded with MOL and tested for antimicrobial, antifungal, germicidal, phytotoxic, insecticidal, cytotoxic, and anti-inflammatory activities. The isolates, MOL1, MOL16, MOL19, and MOL21, possessed antibacterial activity against Staphylococcus aureus, whereas MOL7 inhibited 55% of the growth of Escherichia coli. MOL3 inhibited the growth of E. coli, S. aureus, and Pseudomonas aeruginosa. The strains, MOL1 and MOL7, showed antifungal activity against Candida albicans and Saccharomyces cerevisiae, while the strains, MOL11 and MOL17, showed activity against Verticillium chlamydosporium. The isolates, MOL3, MOL7, MOL9, MOL15, MOL17, MOL18, and MOL19, inhibited the growth of Lemna minor (duckweed) at 100 μg/ml. MOL2 exhibited strong activity in the brine shrimp assay, while MOL1, MOL2, MOL5, MOL6, MOL12, MOL17, MOL19, and MOL20 showed insecticidal, and MOL3 demonstrated larvicidal and antileishmanial activity. The isolated potent endophytes were identified as Aspergillus, Penicillium, Fusarium, Tricoderma, Rhizoctonia, Mucor, Alternaria, Pestalotiopsis, Acremonium, and Cladosporium through morphological and microscopic characteristics of the colonies.
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spelling pubmed-97799992022-12-23 Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves Rehman, Bushra Khan, Sumera Afzal Hamayun, Muhammad Iqbal, Amjad Lee, In-Jung Biomed Res Int Research Article Plant species are known to harbor large number of endophytes, which stays in plant tissues as symbionts. These endophytes secrete large array of bioactive compounds that have potency against certain diseases with no side effects. We have collected leaf samples of the Moringa oleifera plant from the Pakistan Forest Institute, Khyber Pakhtunkhwa, Pakistan for the isolation of beneficial endophytes. The strains isolated from the leaves of M. oleifera were coded with MOL and tested for antimicrobial, antifungal, germicidal, phytotoxic, insecticidal, cytotoxic, and anti-inflammatory activities. The isolates, MOL1, MOL16, MOL19, and MOL21, possessed antibacterial activity against Staphylococcus aureus, whereas MOL7 inhibited 55% of the growth of Escherichia coli. MOL3 inhibited the growth of E. coli, S. aureus, and Pseudomonas aeruginosa. The strains, MOL1 and MOL7, showed antifungal activity against Candida albicans and Saccharomyces cerevisiae, while the strains, MOL11 and MOL17, showed activity against Verticillium chlamydosporium. The isolates, MOL3, MOL7, MOL9, MOL15, MOL17, MOL18, and MOL19, inhibited the growth of Lemna minor (duckweed) at 100 μg/ml. MOL2 exhibited strong activity in the brine shrimp assay, while MOL1, MOL2, MOL5, MOL6, MOL12, MOL17, MOL19, and MOL20 showed insecticidal, and MOL3 demonstrated larvicidal and antileishmanial activity. The isolated potent endophytes were identified as Aspergillus, Penicillium, Fusarium, Tricoderma, Rhizoctonia, Mucor, Alternaria, Pestalotiopsis, Acremonium, and Cladosporium through morphological and microscopic characteristics of the colonies. Hindawi 2022-12-15 /pmc/articles/PMC9779999/ /pubmed/36567913 http://dx.doi.org/10.1155/2022/2461021 Text en Copyright © 2022 Bushra Rehman et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rehman, Bushra
Khan, Sumera Afzal
Hamayun, Muhammad
Iqbal, Amjad
Lee, In-Jung
Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves
title Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves
title_full Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves
title_fullStr Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves
title_full_unstemmed Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves
title_short Potent Bioactivity of Endophytic Fungi Isolated from Moringa oleifera Leaves
title_sort potent bioactivity of endophytic fungi isolated from moringa oleifera leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9779999/
https://www.ncbi.nlm.nih.gov/pubmed/36567913
http://dx.doi.org/10.1155/2022/2461021
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