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Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC
Aβ(25-35)-induced PC12 cells were used as the in vitro injury model to evaluate the effects on PC12 cells after intervention with the “ginseng–polygala” drug pair. The results showed that the drug pair could significantly increase cell activity and reduce the level of reactive oxygen species and the...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780267/ https://www.ncbi.nlm.nih.gov/pubmed/36569314 http://dx.doi.org/10.3389/fphar.2022.949757 |
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author | Wang, Zhengyang Sun, Xiaomeng Zhao, Yue Ga, Liba Li, Qi Li, Qian Wang, Xiaotong Yang, Chunjuan |
author_facet | Wang, Zhengyang Sun, Xiaomeng Zhao, Yue Ga, Liba Li, Qi Li, Qian Wang, Xiaotong Yang, Chunjuan |
author_sort | Wang, Zhengyang |
collection | PubMed |
description | Aβ(25-35)-induced PC12 cells were used as the in vitro injury model to evaluate the effects on PC12 cells after intervention with the “ginseng–polygala” drug pair. The results showed that the drug pair could significantly increase cell activity and reduce the level of reactive oxygen species and the concentration of inflammatory factors to improve the Alzheimer’s disease treatment process. Furthermore, to rapidly identify and classify complicated bioactive components of the drug pair, a liquid chromatography with quadrupole time-of-flight mass spectrometry method combined with a molecular network strategy was established. With this strategy, 40 constituents were preliminarily identified and a database of the compounds was successfully established. Among them, 12 compounds of different categories were accurately identified by comparison with reference substances. The content of the aforementioned active components was simultaneously determined by HPLC to control the quality of compatible medicinal materials, and the verification results of the analytical method met the content determination requirements. The results revealed that after compatibility, the content change of the components is not the simple addition of quantity but the comprehensive effect of the two medicines. In conclusion, this study could provide a generally applicable strategy for pharmacological activity, structural identification, and content determination in traditional Chinese medicine and its compatibility. |
format | Online Article Text |
id | pubmed-9780267 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97802672022-12-24 Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC Wang, Zhengyang Sun, Xiaomeng Zhao, Yue Ga, Liba Li, Qi Li, Qian Wang, Xiaotong Yang, Chunjuan Front Pharmacol Pharmacology Aβ(25-35)-induced PC12 cells were used as the in vitro injury model to evaluate the effects on PC12 cells after intervention with the “ginseng–polygala” drug pair. The results showed that the drug pair could significantly increase cell activity and reduce the level of reactive oxygen species and the concentration of inflammatory factors to improve the Alzheimer’s disease treatment process. Furthermore, to rapidly identify and classify complicated bioactive components of the drug pair, a liquid chromatography with quadrupole time-of-flight mass spectrometry method combined with a molecular network strategy was established. With this strategy, 40 constituents were preliminarily identified and a database of the compounds was successfully established. Among them, 12 compounds of different categories were accurately identified by comparison with reference substances. The content of the aforementioned active components was simultaneously determined by HPLC to control the quality of compatible medicinal materials, and the verification results of the analytical method met the content determination requirements. The results revealed that after compatibility, the content change of the components is not the simple addition of quantity but the comprehensive effect of the two medicines. In conclusion, this study could provide a generally applicable strategy for pharmacological activity, structural identification, and content determination in traditional Chinese medicine and its compatibility. Frontiers Media S.A. 2022-12-09 /pmc/articles/PMC9780267/ /pubmed/36569314 http://dx.doi.org/10.3389/fphar.2022.949757 Text en Copyright © 2022 Wang, Sun, Zhao, Ga, Li, Li, Wang and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Wang, Zhengyang Sun, Xiaomeng Zhao, Yue Ga, Liba Li, Qi Li, Qian Wang, Xiaotong Yang, Chunjuan Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC |
title | Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC |
title_full | Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC |
title_fullStr | Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC |
title_full_unstemmed | Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC |
title_short | Qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against PC12 cell injury based on UHPLC-QTOF-MS and HPLC |
title_sort | qualitative and quantitative analysis of the bioactive components of “ginseng–polygala” drug pair against pc12 cell injury based on uhplc-qtof-ms and hplc |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780267/ https://www.ncbi.nlm.nih.gov/pubmed/36569314 http://dx.doi.org/10.3389/fphar.2022.949757 |
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