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High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease

INTRODUCTION: Alzheimer’s (AD) and Parkinson’s disease (PD) are neurodegenerative conditions characterized by incremental deposition of β-amyloid (Aβ) and α-synuclein in AD and PD brain, respectively, in relatively conserved patterns. Both are associated with neuroinflammation, with a proposed micro...

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Autores principales: Emery, David C., Davies, Maria, Cerajewska, Tanya L., Taylor, Jelena, Hazell, Mae, Paterson, Alex, Allen-Birt, Shelley J., West, Nicola X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780557/
https://www.ncbi.nlm.nih.gov/pubmed/36570533
http://dx.doi.org/10.3389/fnagi.2022.1026260
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author Emery, David C.
Davies, Maria
Cerajewska, Tanya L.
Taylor, Jelena
Hazell, Mae
Paterson, Alex
Allen-Birt, Shelley J.
West, Nicola X.
author_facet Emery, David C.
Davies, Maria
Cerajewska, Tanya L.
Taylor, Jelena
Hazell, Mae
Paterson, Alex
Allen-Birt, Shelley J.
West, Nicola X.
author_sort Emery, David C.
collection PubMed
description INTRODUCTION: Alzheimer’s (AD) and Parkinson’s disease (PD) are neurodegenerative conditions characterized by incremental deposition of β-amyloid (Aβ) and α-synuclein in AD and PD brain, respectively, in relatively conserved patterns. Both are associated with neuroinflammation, with a proposed microbial component for disease initiation and/or progression. Notably, Aβ and α-synuclein have been shown to possess antimicrobial properties. There is evidence for bacterial presence within the brain, including the oral pathobiont Porphyromonas gingivalis, with cognitive impairment and brain pathology being linked to periodontal (gum) disease and gut dysbiosis. METHODS: Here, we use high resolution 16S rRNA PCR-based Next Generation Sequencing (16SNGS) to characterize bacterial composition in brain areas associated with the early, intermediate and late-stage of the diseases. RESULTS AND DISCUSSION: This study reveals the widespread presence of bacteria in areas of the brain associated with AD and PD pathology, with distinctly different bacterial profiles in blood and brain. Brain area profiles were overall somewhat similar, predominantly oral, with some bacteria subgingival and oronasal in origin, and relatively comparable profiles in AD and PD brain. However, brain areas associated with early disease development, such as the locus coeruleus, were substantially different in bacterial DNA content compared to areas affected later in disease etiology.
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spelling pubmed-97805572022-12-24 High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease Emery, David C. Davies, Maria Cerajewska, Tanya L. Taylor, Jelena Hazell, Mae Paterson, Alex Allen-Birt, Shelley J. West, Nicola X. Front Aging Neurosci Aging Neuroscience INTRODUCTION: Alzheimer’s (AD) and Parkinson’s disease (PD) are neurodegenerative conditions characterized by incremental deposition of β-amyloid (Aβ) and α-synuclein in AD and PD brain, respectively, in relatively conserved patterns. Both are associated with neuroinflammation, with a proposed microbial component for disease initiation and/or progression. Notably, Aβ and α-synuclein have been shown to possess antimicrobial properties. There is evidence for bacterial presence within the brain, including the oral pathobiont Porphyromonas gingivalis, with cognitive impairment and brain pathology being linked to periodontal (gum) disease and gut dysbiosis. METHODS: Here, we use high resolution 16S rRNA PCR-based Next Generation Sequencing (16SNGS) to characterize bacterial composition in brain areas associated with the early, intermediate and late-stage of the diseases. RESULTS AND DISCUSSION: This study reveals the widespread presence of bacteria in areas of the brain associated with AD and PD pathology, with distinctly different bacterial profiles in blood and brain. Brain area profiles were overall somewhat similar, predominantly oral, with some bacteria subgingival and oronasal in origin, and relatively comparable profiles in AD and PD brain. However, brain areas associated with early disease development, such as the locus coeruleus, were substantially different in bacterial DNA content compared to areas affected later in disease etiology. Frontiers Media S.A. 2022-12-09 /pmc/articles/PMC9780557/ /pubmed/36570533 http://dx.doi.org/10.3389/fnagi.2022.1026260 Text en Copyright © 2022 Emery, Davies, Cerajewska, Taylor, Hazell, Paterson, Allen-Birt and West. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging Neuroscience
Emery, David C.
Davies, Maria
Cerajewska, Tanya L.
Taylor, Jelena
Hazell, Mae
Paterson, Alex
Allen-Birt, Shelley J.
West, Nicola X.
High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease
title High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease
title_full High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease
title_fullStr High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease
title_full_unstemmed High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease
title_short High resolution 16S rRNA gene Next Generation Sequencing study of brain areas associated with Alzheimer’s and Parkinson’s disease
title_sort high resolution 16s rrna gene next generation sequencing study of brain areas associated with alzheimer’s and parkinson’s disease
topic Aging Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780557/
https://www.ncbi.nlm.nih.gov/pubmed/36570533
http://dx.doi.org/10.3389/fnagi.2022.1026260
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