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The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)

INTRODUCTION: Most of elite cultivated grapevine varieties (Vitis vinifera L.), conventionally grafted on rootstocks, are becoming more and more affected by climate changes, such as increase of salinity. Therefore, we revisited the valuable genetic resources of wild grapevines (V. sylvestris) to ela...

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Autores principales: Daldoul, Samia, Hanzouli, Faouzia, Hamdi, Zohra, Chenenaoui, Synda, Wetzel, Thierry, Nick, Peter, Mliki, Ahmed, Gargouri, Mahmoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780605/
https://www.ncbi.nlm.nih.gov/pubmed/36570937
http://dx.doi.org/10.3389/fpls.2022.1077710
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author Daldoul, Samia
Hanzouli, Faouzia
Hamdi, Zohra
Chenenaoui, Synda
Wetzel, Thierry
Nick, Peter
Mliki, Ahmed
Gargouri, Mahmoud
author_facet Daldoul, Samia
Hanzouli, Faouzia
Hamdi, Zohra
Chenenaoui, Synda
Wetzel, Thierry
Nick, Peter
Mliki, Ahmed
Gargouri, Mahmoud
author_sort Daldoul, Samia
collection PubMed
description INTRODUCTION: Most of elite cultivated grapevine varieties (Vitis vinifera L.), conventionally grafted on rootstocks, are becoming more and more affected by climate changes, such as increase of salinity. Therefore, we revisited the valuable genetic resources of wild grapevines (V. sylvestris) to elaborate strategies for a sustainable viticulture. METHODS: Here, we compared physiological and biochemical responses of two salt-tolerant species: a wild grapevine genotype “Tebaba” from our previous studies and the conventional rootstock “1103 Paulsen”. Interestingly, our physio-biochemical results showed that under 150mM NaCl, “Tebaba” maintains higher leaf osmotic potential, lower Na+/K+ ratio and a significant peaked increase of polyphenol content at the first 8h of salinity stress. This behavior allowed to hypothesis a drastic repatterning of metabolism in “Tebaba’s” roots following a biphasic response. In order to deepen our understanding on the “Tebaba” salt tolerance mechanism, we investigated a time-dependent transcriptomic analysis covering three sampling times, 8h, 24h and 48h. RESULTS: The dynamic analysis indicated that “Tebaba” root cells detect and respond on a large scale within 8h to an accumulation of ROS by enhancing a translational reprogramming process and inducing the transcripts of glycolytic metabolism and flavonoids biosynthesis as a predominate non-enzymatic scavenging process. Afterwards, there is a transition to a largely gluconeogenic stage followed by a combined response mechanism based on cell wall remodeling and lignin biosynthesis with an efficient osmoregulation between 24 and 48 h. DISCUSSION: This investigation explored for the first time in depth the established cross-talk between the physiological, biochemical and transcriptional regulators contributing to propose a hypothetical model of the dynamic salt mechanism tolerance of wild grapevines. In summary, these findings allowed further understanding of the genetic regulation mechanism of salt-tolerance in V. sylvestris and identified specific candidate genes valuable for appropriate breeding strategies.
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spelling pubmed-97806052022-12-24 The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris) Daldoul, Samia Hanzouli, Faouzia Hamdi, Zohra Chenenaoui, Synda Wetzel, Thierry Nick, Peter Mliki, Ahmed Gargouri, Mahmoud Front Plant Sci Plant Science INTRODUCTION: Most of elite cultivated grapevine varieties (Vitis vinifera L.), conventionally grafted on rootstocks, are becoming more and more affected by climate changes, such as increase of salinity. Therefore, we revisited the valuable genetic resources of wild grapevines (V. sylvestris) to elaborate strategies for a sustainable viticulture. METHODS: Here, we compared physiological and biochemical responses of two salt-tolerant species: a wild grapevine genotype “Tebaba” from our previous studies and the conventional rootstock “1103 Paulsen”. Interestingly, our physio-biochemical results showed that under 150mM NaCl, “Tebaba” maintains higher leaf osmotic potential, lower Na+/K+ ratio and a significant peaked increase of polyphenol content at the first 8h of salinity stress. This behavior allowed to hypothesis a drastic repatterning of metabolism in “Tebaba’s” roots following a biphasic response. In order to deepen our understanding on the “Tebaba” salt tolerance mechanism, we investigated a time-dependent transcriptomic analysis covering three sampling times, 8h, 24h and 48h. RESULTS: The dynamic analysis indicated that “Tebaba” root cells detect and respond on a large scale within 8h to an accumulation of ROS by enhancing a translational reprogramming process and inducing the transcripts of glycolytic metabolism and flavonoids biosynthesis as a predominate non-enzymatic scavenging process. Afterwards, there is a transition to a largely gluconeogenic stage followed by a combined response mechanism based on cell wall remodeling and lignin biosynthesis with an efficient osmoregulation between 24 and 48 h. DISCUSSION: This investigation explored for the first time in depth the established cross-talk between the physiological, biochemical and transcriptional regulators contributing to propose a hypothetical model of the dynamic salt mechanism tolerance of wild grapevines. In summary, these findings allowed further understanding of the genetic regulation mechanism of salt-tolerance in V. sylvestris and identified specific candidate genes valuable for appropriate breeding strategies. Frontiers Media S.A. 2022-12-09 /pmc/articles/PMC9780605/ /pubmed/36570937 http://dx.doi.org/10.3389/fpls.2022.1077710 Text en Copyright © 2022 Daldoul, Hanzouli, Hamdi, Chenenaoui, Wetzel, Nick, Mliki and Gargouri https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Daldoul, Samia
Hanzouli, Faouzia
Hamdi, Zohra
Chenenaoui, Synda
Wetzel, Thierry
Nick, Peter
Mliki, Ahmed
Gargouri, Mahmoud
The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)
title The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)
title_full The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)
title_fullStr The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)
title_full_unstemmed The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)
title_short The root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (Vitis vinifera subsp. sylvestris)
title_sort root transcriptome dynamics reveals new valuable insights in the salt-resilience mechanism of wild grapevine (vitis vinifera subsp. sylvestris)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780605/
https://www.ncbi.nlm.nih.gov/pubmed/36570937
http://dx.doi.org/10.3389/fpls.2022.1077710
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