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Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital
ABSTRACT: COVID-19 patients have often required prolonged endotracheal intubation, increasing the risk of developing ventilator-associated pneumonia (VAP). A preventive strategy is proposed based on an endotracheal tube (ETT) modified by the in situ deposition of eucalyptus-mediated synthesized silv...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780629/ https://www.ncbi.nlm.nih.gov/pubmed/36562803 http://dx.doi.org/10.1007/s00253-022-12327-w |
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author | Lethongkam, Sakkarin Sunghan, Jutapoln Wangdee, Chalika Durongphongtorn, Sumit Siri, Ratchaneewan Wunnoo, Suttiwan Paosen, Supakit Voravuthikunchai, Supayang P. Dejyong, Krittee Daengngam, Chalongrat |
author_facet | Lethongkam, Sakkarin Sunghan, Jutapoln Wangdee, Chalika Durongphongtorn, Sumit Siri, Ratchaneewan Wunnoo, Suttiwan Paosen, Supakit Voravuthikunchai, Supayang P. Dejyong, Krittee Daengngam, Chalongrat |
author_sort | Lethongkam, Sakkarin |
collection | PubMed |
description | ABSTRACT: COVID-19 patients have often required prolonged endotracheal intubation, increasing the risk of developing ventilator-associated pneumonia (VAP). A preventive strategy is proposed based on an endotracheal tube (ETT) modified by the in situ deposition of eucalyptus-mediated synthesized silver nanoparticles (AgNPs). The surfaces of the modified ETT were embedded with AgNPs of approximately 28 nm and presented a nanoscale roughness. Energy dispersive X-ray spectroscopy confirmed the presence of silver on and inside the coated ETT, which exhibited excellent antimicrobial activity against Gram-positive and Gram-negative bacteria, and fungi, including multidrug-resistant clinical isolates. Inhibition of planktonic growth and microbial adhesion ranged from 99 to 99.999% without cytotoxic effects on mammalian cells. Kinetic studies showed that microbial adhesion to the coated surface was inhibited within 2 h. Cell viability in biofilms supplemented with human tracheal mucus was reduced by up to 95%. In a porcine VAP model, the AgNPs-coated ETT prevented adhesion of Pseudomonas aeruginosa and completely inhibited bacterial invasion of lung tissue. The potential antimicrobial efficacy and safety of the coated ETT were established in a randomized control trial involving 47 veterinary patients. The microbial burden was significantly lower on the surface of the AgNPs-coated ETT than on the uncoated ETT (p < 0.05). KEY POINTS: • Endotracheal tube surfaces were modified by coating with green-synthesized AgNPs • P. aeruginosa burden of endotracheal tube and lung was reduced in a porcine model • Effective antimicrobial activity and safety was demonstrated in a clinical trial SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-12327-w. |
format | Online Article Text |
id | pubmed-9780629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-97806292022-12-23 Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital Lethongkam, Sakkarin Sunghan, Jutapoln Wangdee, Chalika Durongphongtorn, Sumit Siri, Ratchaneewan Wunnoo, Suttiwan Paosen, Supakit Voravuthikunchai, Supayang P. Dejyong, Krittee Daengngam, Chalongrat Appl Microbiol Biotechnol Biotechnological Products and Process Engineering ABSTRACT: COVID-19 patients have often required prolonged endotracheal intubation, increasing the risk of developing ventilator-associated pneumonia (VAP). A preventive strategy is proposed based on an endotracheal tube (ETT) modified by the in situ deposition of eucalyptus-mediated synthesized silver nanoparticles (AgNPs). The surfaces of the modified ETT were embedded with AgNPs of approximately 28 nm and presented a nanoscale roughness. Energy dispersive X-ray spectroscopy confirmed the presence of silver on and inside the coated ETT, which exhibited excellent antimicrobial activity against Gram-positive and Gram-negative bacteria, and fungi, including multidrug-resistant clinical isolates. Inhibition of planktonic growth and microbial adhesion ranged from 99 to 99.999% without cytotoxic effects on mammalian cells. Kinetic studies showed that microbial adhesion to the coated surface was inhibited within 2 h. Cell viability in biofilms supplemented with human tracheal mucus was reduced by up to 95%. In a porcine VAP model, the AgNPs-coated ETT prevented adhesion of Pseudomonas aeruginosa and completely inhibited bacterial invasion of lung tissue. The potential antimicrobial efficacy and safety of the coated ETT were established in a randomized control trial involving 47 veterinary patients. The microbial burden was significantly lower on the surface of the AgNPs-coated ETT than on the uncoated ETT (p < 0.05). KEY POINTS: • Endotracheal tube surfaces were modified by coating with green-synthesized AgNPs • P. aeruginosa burden of endotracheal tube and lung was reduced in a porcine model • Effective antimicrobial activity and safety was demonstrated in a clinical trial SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-022-12327-w. Springer Berlin Heidelberg 2022-12-23 2023 /pmc/articles/PMC9780629/ /pubmed/36562803 http://dx.doi.org/10.1007/s00253-022-12327-w Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Biotechnological Products and Process Engineering Lethongkam, Sakkarin Sunghan, Jutapoln Wangdee, Chalika Durongphongtorn, Sumit Siri, Ratchaneewan Wunnoo, Suttiwan Paosen, Supakit Voravuthikunchai, Supayang P. Dejyong, Krittee Daengngam, Chalongrat Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
title | Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
title_full | Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
title_fullStr | Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
title_full_unstemmed | Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
title_short | Biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
title_sort | biogenic nanosilver-fabricated endotracheal tube to prevent microbial colonization in a veterinary hospital |
topic | Biotechnological Products and Process Engineering |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780629/ https://www.ncbi.nlm.nih.gov/pubmed/36562803 http://dx.doi.org/10.1007/s00253-022-12327-w |
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