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Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology

Ophiocordyceps sinensis is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of O. sinensis and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein fro...

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Autores principales: Tang, Chu-Yu, Wang, Jie, Liu, Xin, Chen, Jian-Bo, Liang, Jing, Wang, Tao, Simpson, Wayne Roydon, Li, Yu-Ling, Li, Xiu-Zhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780674/
https://www.ncbi.nlm.nih.gov/pubmed/36569047
http://dx.doi.org/10.3389/fmicb.2022.1055055
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author Tang, Chu-Yu
Wang, Jie
Liu, Xin
Chen, Jian-Bo
Liang, Jing
Wang, Tao
Simpson, Wayne Roydon
Li, Yu-Ling
Li, Xiu-Zhang
author_facet Tang, Chu-Yu
Wang, Jie
Liu, Xin
Chen, Jian-Bo
Liang, Jing
Wang, Tao
Simpson, Wayne Roydon
Li, Yu-Ling
Li, Xiu-Zhang
author_sort Tang, Chu-Yu
collection PubMed
description Ophiocordyceps sinensis is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of O. sinensis and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein from O. sinensis using response surface methodology (RSM) and investigate the increased mycelial protein content using transcriptomics. The maximum mycelial protein content of 2.11% was obtained using a medium consisting of 20% beef broth, 0.10% peptone, 2% glucose, 0.15% yeast extract, 0.20% KH(2)PO(4), and 0.02% MgSO(4). Transcriptome analysis identified 790 differentially expressed genes (DEGs), including 592 up-regulated genes and 198 down-regulated genes, optimisation resulted in more up-regulated genes. The main DEGs were enriched in metabolic pathways, ABC transporters, starch and sucrose metabolism, tyrosine metabolism, and glutathione metabolism. In addition, some DEGs associated with mycelial protein enhancement such as tyrosinase (TYR), glutathione S-transferase (GST), glutamine synthetase (glnA), and β-glucosidase may contribute to increased mycelial protein content. Real-time quantitative PCR (RT-qPCR) was used to confirm gene expression and the results support the accuracy of RNA-Seq and DEG analysis. This study provides an optimized fermentation method for enhancing the mycelial protein content of O. sinensis and a reference for the effective development of O. sinensis protein.
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spelling pubmed-97806742022-12-24 Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology Tang, Chu-Yu Wang, Jie Liu, Xin Chen, Jian-Bo Liang, Jing Wang, Tao Simpson, Wayne Roydon Li, Yu-Ling Li, Xiu-Zhang Front Microbiol Microbiology Ophiocordyceps sinensis is widely utilized due to its pharmaceutical value. Mycelial protein forms a key active component of O. sinensis and determines the medicinal potential of fungus. Here, we describe the development of an optimized fermentation medium to obtain more mycelial soluble protein from O. sinensis using response surface methodology (RSM) and investigate the increased mycelial protein content using transcriptomics. The maximum mycelial protein content of 2.11% was obtained using a medium consisting of 20% beef broth, 0.10% peptone, 2% glucose, 0.15% yeast extract, 0.20% KH(2)PO(4), and 0.02% MgSO(4). Transcriptome analysis identified 790 differentially expressed genes (DEGs), including 592 up-regulated genes and 198 down-regulated genes, optimisation resulted in more up-regulated genes. The main DEGs were enriched in metabolic pathways, ABC transporters, starch and sucrose metabolism, tyrosine metabolism, and glutathione metabolism. In addition, some DEGs associated with mycelial protein enhancement such as tyrosinase (TYR), glutathione S-transferase (GST), glutamine synthetase (glnA), and β-glucosidase may contribute to increased mycelial protein content. Real-time quantitative PCR (RT-qPCR) was used to confirm gene expression and the results support the accuracy of RNA-Seq and DEG analysis. This study provides an optimized fermentation method for enhancing the mycelial protein content of O. sinensis and a reference for the effective development of O. sinensis protein. Frontiers Media S.A. 2022-12-09 /pmc/articles/PMC9780674/ /pubmed/36569047 http://dx.doi.org/10.3389/fmicb.2022.1055055 Text en Copyright © 2022 Tang, Wang, Liu, Chen, Liang, Wang, Simpson, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tang, Chu-Yu
Wang, Jie
Liu, Xin
Chen, Jian-Bo
Liang, Jing
Wang, Tao
Simpson, Wayne Roydon
Li, Yu-Ling
Li, Xiu-Zhang
Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
title Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
title_full Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
title_fullStr Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
title_full_unstemmed Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
title_short Medium optimization for high mycelial soluble protein content of Ophiocordyceps sinensis using response surface methodology
title_sort medium optimization for high mycelial soluble protein content of ophiocordyceps sinensis using response surface methodology
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780674/
https://www.ncbi.nlm.nih.gov/pubmed/36569047
http://dx.doi.org/10.3389/fmicb.2022.1055055
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