Cargando…

Simulation of Rapid Thermal Cycle for Ultra-Fast PCR

The polymerase chain reaction (PCR) technology is a mainstream detection method used in medical diagnoses, environmental monitoring, food hygiene, and safety. However, the systematic analysis of a compact structure with fast temperature changes for an ultra-fast PCR device that is convenient for on-...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Zhuo, Zhang, Jiali, Tong, Xin, Li, Wenbing, Liang, Lijuan, Liu, Bo, Chen, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780856/
https://www.ncbi.nlm.nih.gov/pubmed/36560360
http://dx.doi.org/10.3390/s22249990
_version_ 1784856931492429824
author Yang, Zhuo
Zhang, Jiali
Tong, Xin
Li, Wenbing
Liang, Lijuan
Liu, Bo
Chen, Chang
author_facet Yang, Zhuo
Zhang, Jiali
Tong, Xin
Li, Wenbing
Liang, Lijuan
Liu, Bo
Chen, Chang
author_sort Yang, Zhuo
collection PubMed
description The polymerase chain reaction (PCR) technology is a mainstream detection method used in medical diagnoses, environmental monitoring, food hygiene, and safety. However, the systematic analysis of a compact structure with fast temperature changes for an ultra-fast PCR device that is convenient for on-site detection still lacks investigation. To overcome the problems of low heating efficiency and non-portability of PCR devices currently used, a miniaturized PCR system based on a microfluidic chip, i.e., lab-on-chip technology, has been proposed. The main objective of this paper is to explore the feasibility of using a heat resistor that can reach a fast heating rate and temperature uniformity combined with air cooling technology for rapid cooling and to investigate the influences of various pattern designs and thicknesses of the resistor on heating rates and temperature uniformity. Additionally, a PCR chip made of various materials with different thermal properties, such as surface emissivity, thermal conductivity, mass density, and heat capacity at constant pressure is analyzed. In addition to the heat loss caused by the natural convection of air, the radiation loss of the simulation object is also considered, which makes the model much closer to the practical situation. Our research results provide a considerable reference for the design of the heating and cooling modules used in the ultra-fast PCR protocol, which has great potential in In Vitro Diagnosis (IVD) and the PCR detection of foodborne pathogens and bacteria.
format Online
Article
Text
id pubmed-9780856
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97808562022-12-24 Simulation of Rapid Thermal Cycle for Ultra-Fast PCR Yang, Zhuo Zhang, Jiali Tong, Xin Li, Wenbing Liang, Lijuan Liu, Bo Chen, Chang Sensors (Basel) Communication The polymerase chain reaction (PCR) technology is a mainstream detection method used in medical diagnoses, environmental monitoring, food hygiene, and safety. However, the systematic analysis of a compact structure with fast temperature changes for an ultra-fast PCR device that is convenient for on-site detection still lacks investigation. To overcome the problems of low heating efficiency and non-portability of PCR devices currently used, a miniaturized PCR system based on a microfluidic chip, i.e., lab-on-chip technology, has been proposed. The main objective of this paper is to explore the feasibility of using a heat resistor that can reach a fast heating rate and temperature uniformity combined with air cooling technology for rapid cooling and to investigate the influences of various pattern designs and thicknesses of the resistor on heating rates and temperature uniformity. Additionally, a PCR chip made of various materials with different thermal properties, such as surface emissivity, thermal conductivity, mass density, and heat capacity at constant pressure is analyzed. In addition to the heat loss caused by the natural convection of air, the radiation loss of the simulation object is also considered, which makes the model much closer to the practical situation. Our research results provide a considerable reference for the design of the heating and cooling modules used in the ultra-fast PCR protocol, which has great potential in In Vitro Diagnosis (IVD) and the PCR detection of foodborne pathogens and bacteria. MDPI 2022-12-18 /pmc/articles/PMC9780856/ /pubmed/36560360 http://dx.doi.org/10.3390/s22249990 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Yang, Zhuo
Zhang, Jiali
Tong, Xin
Li, Wenbing
Liang, Lijuan
Liu, Bo
Chen, Chang
Simulation of Rapid Thermal Cycle for Ultra-Fast PCR
title Simulation of Rapid Thermal Cycle for Ultra-Fast PCR
title_full Simulation of Rapid Thermal Cycle for Ultra-Fast PCR
title_fullStr Simulation of Rapid Thermal Cycle for Ultra-Fast PCR
title_full_unstemmed Simulation of Rapid Thermal Cycle for Ultra-Fast PCR
title_short Simulation of Rapid Thermal Cycle for Ultra-Fast PCR
title_sort simulation of rapid thermal cycle for ultra-fast pcr
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780856/
https://www.ncbi.nlm.nih.gov/pubmed/36560360
http://dx.doi.org/10.3390/s22249990
work_keys_str_mv AT yangzhuo simulationofrapidthermalcycleforultrafastpcr
AT zhangjiali simulationofrapidthermalcycleforultrafastpcr
AT tongxin simulationofrapidthermalcycleforultrafastpcr
AT liwenbing simulationofrapidthermalcycleforultrafastpcr
AT lianglijuan simulationofrapidthermalcycleforultrafastpcr
AT liubo simulationofrapidthermalcycleforultrafastpcr
AT chenchang simulationofrapidthermalcycleforultrafastpcr