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Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses
Porcine epidemic diarrhea virus (PEDV), belonging to the genus Alphacoronavirus, can cause serious disease in pigs of all ages, especially in suckling pigs. Differences in virulence have been observed between various strains of this virus. In this study, four pigs were inoculated with PEDV from Germ...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780976/ https://www.ncbi.nlm.nih.gov/pubmed/36560755 http://dx.doi.org/10.3390/v14122751 |
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author | Lazov, Christina M. Lohse, Louise Belsham, Graham J. Rasmussen, Thomas Bruun Bøtner, Anette |
author_facet | Lazov, Christina M. Lohse, Louise Belsham, Graham J. Rasmussen, Thomas Bruun Bøtner, Anette |
author_sort | Lazov, Christina M. |
collection | PubMed |
description | Porcine epidemic diarrhea virus (PEDV), belonging to the genus Alphacoronavirus, can cause serious disease in pigs of all ages, especially in suckling pigs. Differences in virulence have been observed between various strains of this virus. In this study, four pigs were inoculated with PEDV from Germany (intestine/intestinal content collected from pigs in 2016) and four pigs with PEDV from Italy (intestine/intestinal material collected from pigs in 2016). The pigs were re-inoculated with the same virus on multiple occasions to create a more robust infection and enhance the antibody responses. The clinical signs and pathological changes observed were generally mild. Two distinct peaks of virus excretion were seen in the group of pigs inoculated with the PEDV from Germany, while only one strong peak was seen for the group of pigs that received the virus from Italy. Seroconversion was seen by days 18 and 10 post-inoculation with PEDV in all surviving pigs from the groups that received the inoculums from Germany and Italy, respectively. Attempts to infect pigs with a swine enteric coronavirus (SeCoV) from Slovakia were unsuccessful, and no signs of infection were observed in the inoculated animals. |
format | Online Article Text |
id | pubmed-9780976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97809762022-12-24 Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses Lazov, Christina M. Lohse, Louise Belsham, Graham J. Rasmussen, Thomas Bruun Bøtner, Anette Viruses Article Porcine epidemic diarrhea virus (PEDV), belonging to the genus Alphacoronavirus, can cause serious disease in pigs of all ages, especially in suckling pigs. Differences in virulence have been observed between various strains of this virus. In this study, four pigs were inoculated with PEDV from Germany (intestine/intestinal content collected from pigs in 2016) and four pigs with PEDV from Italy (intestine/intestinal material collected from pigs in 2016). The pigs were re-inoculated with the same virus on multiple occasions to create a more robust infection and enhance the antibody responses. The clinical signs and pathological changes observed were generally mild. Two distinct peaks of virus excretion were seen in the group of pigs inoculated with the PEDV from Germany, while only one strong peak was seen for the group of pigs that received the virus from Italy. Seroconversion was seen by days 18 and 10 post-inoculation with PEDV in all surviving pigs from the groups that received the inoculums from Germany and Italy, respectively. Attempts to infect pigs with a swine enteric coronavirus (SeCoV) from Slovakia were unsuccessful, and no signs of infection were observed in the inoculated animals. MDPI 2022-12-09 /pmc/articles/PMC9780976/ /pubmed/36560755 http://dx.doi.org/10.3390/v14122751 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lazov, Christina M. Lohse, Louise Belsham, Graham J. Rasmussen, Thomas Bruun Bøtner, Anette Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses |
title | Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses |
title_full | Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses |
title_fullStr | Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses |
title_full_unstemmed | Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses |
title_short | Experimental Infection of Pigs with Recent European Porcine Epidemic Diarrhea Viruses |
title_sort | experimental infection of pigs with recent european porcine epidemic diarrhea viruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9780976/ https://www.ncbi.nlm.nih.gov/pubmed/36560755 http://dx.doi.org/10.3390/v14122751 |
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