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Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nu...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781119/ https://www.ncbi.nlm.nih.gov/pubmed/36559017 http://dx.doi.org/10.3390/ph15121567 |
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author | Donadel, Guilherme Dalmagro, Mariana de Oliveira, João Antonio Berta Zardeto, Giuliana Pinc, Mariana Moraes Hoscheid, Jaqueline Alberton, Odair Belettini, Salviano Tramontin Jacomassi, Ezilda Gasparotto Junior, Arquimedes Lourenço, Emerson Luiz Botelho |
author_facet | Donadel, Guilherme Dalmagro, Mariana de Oliveira, João Antonio Berta Zardeto, Giuliana Pinc, Mariana Moraes Hoscheid, Jaqueline Alberton, Odair Belettini, Salviano Tramontin Jacomassi, Ezilda Gasparotto Junior, Arquimedes Lourenço, Emerson Luiz Botelho |
author_sort | Donadel, Guilherme |
collection | PubMed |
description | Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic examination of the vaginal mucosa and reproductive system. A histological examination was also performed. No clinically significant changes were detected during the entire experimental period. All biochemical, hematological, or histopathological parameters were within the normal range for the species. The data obtained allow us to suggest that the E. uniflora vaginal formulations are safe in this experimental model. |
format | Online Article Text |
id | pubmed-9781119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97811192022-12-24 Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats Donadel, Guilherme Dalmagro, Mariana de Oliveira, João Antonio Berta Zardeto, Giuliana Pinc, Mariana Moraes Hoscheid, Jaqueline Alberton, Odair Belettini, Salviano Tramontin Jacomassi, Ezilda Gasparotto Junior, Arquimedes Lourenço, Emerson Luiz Botelho Pharmaceuticals (Basel) Article Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic examination of the vaginal mucosa and reproductive system. A histological examination was also performed. No clinically significant changes were detected during the entire experimental period. All biochemical, hematological, or histopathological parameters were within the normal range for the species. The data obtained allow us to suggest that the E. uniflora vaginal formulations are safe in this experimental model. MDPI 2022-12-15 /pmc/articles/PMC9781119/ /pubmed/36559017 http://dx.doi.org/10.3390/ph15121567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Donadel, Guilherme Dalmagro, Mariana de Oliveira, João Antonio Berta Zardeto, Giuliana Pinc, Mariana Moraes Hoscheid, Jaqueline Alberton, Odair Belettini, Salviano Tramontin Jacomassi, Ezilda Gasparotto Junior, Arquimedes Lourenço, Emerson Luiz Botelho Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats |
title | Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats |
title_full | Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats |
title_fullStr | Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats |
title_full_unstemmed | Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats |
title_short | Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats |
title_sort | safety investigations of two formulations for vaginal use obtained from eugenia uniflora l. leaves in female rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781119/ https://www.ncbi.nlm.nih.gov/pubmed/36559017 http://dx.doi.org/10.3390/ph15121567 |
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