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Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats

Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nu...

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Autores principales: Donadel, Guilherme, Dalmagro, Mariana, de Oliveira, João Antonio Berta, Zardeto, Giuliana, Pinc, Mariana Moraes, Hoscheid, Jaqueline, Alberton, Odair, Belettini, Salviano Tramontin, Jacomassi, Ezilda, Gasparotto Junior, Arquimedes, Lourenço, Emerson Luiz Botelho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781119/
https://www.ncbi.nlm.nih.gov/pubmed/36559017
http://dx.doi.org/10.3390/ph15121567
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author Donadel, Guilherme
Dalmagro, Mariana
de Oliveira, João Antonio Berta
Zardeto, Giuliana
Pinc, Mariana Moraes
Hoscheid, Jaqueline
Alberton, Odair
Belettini, Salviano Tramontin
Jacomassi, Ezilda
Gasparotto Junior, Arquimedes
Lourenço, Emerson Luiz Botelho
author_facet Donadel, Guilherme
Dalmagro, Mariana
de Oliveira, João Antonio Berta
Zardeto, Giuliana
Pinc, Mariana Moraes
Hoscheid, Jaqueline
Alberton, Odair
Belettini, Salviano Tramontin
Jacomassi, Ezilda
Gasparotto Junior, Arquimedes
Lourenço, Emerson Luiz Botelho
author_sort Donadel, Guilherme
collection PubMed
description Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic examination of the vaginal mucosa and reproductive system. A histological examination was also performed. No clinically significant changes were detected during the entire experimental period. All biochemical, hematological, or histopathological parameters were within the normal range for the species. The data obtained allow us to suggest that the E. uniflora vaginal formulations are safe in this experimental model.
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spelling pubmed-97811192022-12-24 Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats Donadel, Guilherme Dalmagro, Mariana de Oliveira, João Antonio Berta Zardeto, Giuliana Pinc, Mariana Moraes Hoscheid, Jaqueline Alberton, Odair Belettini, Salviano Tramontin Jacomassi, Ezilda Gasparotto Junior, Arquimedes Lourenço, Emerson Luiz Botelho Pharmaceuticals (Basel) Article Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic examination of the vaginal mucosa and reproductive system. A histological examination was also performed. No clinically significant changes were detected during the entire experimental period. All biochemical, hematological, or histopathological parameters were within the normal range for the species. The data obtained allow us to suggest that the E. uniflora vaginal formulations are safe in this experimental model. MDPI 2022-12-15 /pmc/articles/PMC9781119/ /pubmed/36559017 http://dx.doi.org/10.3390/ph15121567 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Donadel, Guilherme
Dalmagro, Mariana
de Oliveira, João Antonio Berta
Zardeto, Giuliana
Pinc, Mariana Moraes
Hoscheid, Jaqueline
Alberton, Odair
Belettini, Salviano Tramontin
Jacomassi, Ezilda
Gasparotto Junior, Arquimedes
Lourenço, Emerson Luiz Botelho
Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
title Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
title_full Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
title_fullStr Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
title_full_unstemmed Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
title_short Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats
title_sort safety investigations of two formulations for vaginal use obtained from eugenia uniflora l. leaves in female rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781119/
https://www.ncbi.nlm.nih.gov/pubmed/36559017
http://dx.doi.org/10.3390/ph15121567
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