Cargando…
Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
The frozen embryo transfer (FET) technique has been progressively used more worldwide due to improved culture conditions, as well as enhanced survival rates after vitrification. However, little is known about the effect of the post-thaw blastocyst culture duration prior to transfer on live birth rat...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781174/ https://www.ncbi.nlm.nih.gov/pubmed/36556058 http://dx.doi.org/10.3390/jcm11247444 |
_version_ | 1784857009254825984 |
---|---|
author | Ciaffaglione, Marta Reschini, Marco Balli, Martina Guarneri, Cristina Palermo, Maria Carla Pinna, Monica Pisaturo, Valerio Somigliana, Edgardo Paffoni, Alessio Vigano’, Paola |
author_facet | Ciaffaglione, Marta Reschini, Marco Balli, Martina Guarneri, Cristina Palermo, Maria Carla Pinna, Monica Pisaturo, Valerio Somigliana, Edgardo Paffoni, Alessio Vigano’, Paola |
author_sort | Ciaffaglione, Marta |
collection | PubMed |
description | The frozen embryo transfer (FET) technique has been progressively used more worldwide due to improved culture conditions, as well as enhanced survival rates after vitrification. However, little is known about the effect of the post-thaw blastocyst culture duration prior to transfer on live birth rate in FET cycles. In this retrospective observational study, we evaluated the influence of two distinct post-thaw blastocyst culture spans (2–4 h versus 20–22 h) on clinical pregnancy and live birth rate. A total of n = 1927 frozen–warmed cycles were included in the analysis. Among those, n = 885 warmed blastocysts were cultured for 2–4 h, and n = 1029 were kept in culture for 20–22 h prior to transfer; the remaining blastocysts did not survive the warming protocol. We observed no significant differences in live birth and clinical pregnancy rates between the two groups. The blastocyst morphological evaluation at transfer improved following the longer culture time. No differences between the two groups were found also for gestational and neonatal outcomes. This work shows that different post-thaw embryo culture timings do not negatively impact pregnancy outcomes. Overall, these results are important in the context of the embryological laboratory in order to better organize the workflow and avoid unnecessary timing-related workload. |
format | Online Article Text |
id | pubmed-9781174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97811742022-12-24 Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles Ciaffaglione, Marta Reschini, Marco Balli, Martina Guarneri, Cristina Palermo, Maria Carla Pinna, Monica Pisaturo, Valerio Somigliana, Edgardo Paffoni, Alessio Vigano’, Paola J Clin Med Article The frozen embryo transfer (FET) technique has been progressively used more worldwide due to improved culture conditions, as well as enhanced survival rates after vitrification. However, little is known about the effect of the post-thaw blastocyst culture duration prior to transfer on live birth rate in FET cycles. In this retrospective observational study, we evaluated the influence of two distinct post-thaw blastocyst culture spans (2–4 h versus 20–22 h) on clinical pregnancy and live birth rate. A total of n = 1927 frozen–warmed cycles were included in the analysis. Among those, n = 885 warmed blastocysts were cultured for 2–4 h, and n = 1029 were kept in culture for 20–22 h prior to transfer; the remaining blastocysts did not survive the warming protocol. We observed no significant differences in live birth and clinical pregnancy rates between the two groups. The blastocyst morphological evaluation at transfer improved following the longer culture time. No differences between the two groups were found also for gestational and neonatal outcomes. This work shows that different post-thaw embryo culture timings do not negatively impact pregnancy outcomes. Overall, these results are important in the context of the embryological laboratory in order to better organize the workflow and avoid unnecessary timing-related workload. MDPI 2022-12-15 /pmc/articles/PMC9781174/ /pubmed/36556058 http://dx.doi.org/10.3390/jcm11247444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ciaffaglione, Marta Reschini, Marco Balli, Martina Guarneri, Cristina Palermo, Maria Carla Pinna, Monica Pisaturo, Valerio Somigliana, Edgardo Paffoni, Alessio Vigano’, Paola Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles |
title | Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles |
title_full | Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles |
title_fullStr | Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles |
title_full_unstemmed | Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles |
title_short | Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles |
title_sort | post-thaw day 5 blastocyst culture time prior to transfer does not affect assisted reproduction technology (art) outcomes in frozen-thawed embryo transfer cycles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781174/ https://www.ncbi.nlm.nih.gov/pubmed/36556058 http://dx.doi.org/10.3390/jcm11247444 |
work_keys_str_mv | AT ciaffaglionemarta postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT reschinimarco postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT ballimartina postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT guarnericristina postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT palermomariacarla postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT pinnamonica postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT pisaturovalerio postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT somiglianaedgardo postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT paffonialessio postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles AT viganopaola postthawday5blastocystculturetimepriortotransferdoesnotaffectassistedreproductiontechnologyartoutcomesinfrozenthawedembryotransfercycles |