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Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles

The frozen embryo transfer (FET) technique has been progressively used more worldwide due to improved culture conditions, as well as enhanced survival rates after vitrification. However, little is known about the effect of the post-thaw blastocyst culture duration prior to transfer on live birth rat...

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Autores principales: Ciaffaglione, Marta, Reschini, Marco, Balli, Martina, Guarneri, Cristina, Palermo, Maria Carla, Pinna, Monica, Pisaturo, Valerio, Somigliana, Edgardo, Paffoni, Alessio, Vigano’, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781174/
https://www.ncbi.nlm.nih.gov/pubmed/36556058
http://dx.doi.org/10.3390/jcm11247444
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author Ciaffaglione, Marta
Reschini, Marco
Balli, Martina
Guarneri, Cristina
Palermo, Maria Carla
Pinna, Monica
Pisaturo, Valerio
Somigliana, Edgardo
Paffoni, Alessio
Vigano’, Paola
author_facet Ciaffaglione, Marta
Reschini, Marco
Balli, Martina
Guarneri, Cristina
Palermo, Maria Carla
Pinna, Monica
Pisaturo, Valerio
Somigliana, Edgardo
Paffoni, Alessio
Vigano’, Paola
author_sort Ciaffaglione, Marta
collection PubMed
description The frozen embryo transfer (FET) technique has been progressively used more worldwide due to improved culture conditions, as well as enhanced survival rates after vitrification. However, little is known about the effect of the post-thaw blastocyst culture duration prior to transfer on live birth rate in FET cycles. In this retrospective observational study, we evaluated the influence of two distinct post-thaw blastocyst culture spans (2–4 h versus 20–22 h) on clinical pregnancy and live birth rate. A total of n = 1927 frozen–warmed cycles were included in the analysis. Among those, n = 885 warmed blastocysts were cultured for 2–4 h, and n = 1029 were kept in culture for 20–22 h prior to transfer; the remaining blastocysts did not survive the warming protocol. We observed no significant differences in live birth and clinical pregnancy rates between the two groups. The blastocyst morphological evaluation at transfer improved following the longer culture time. No differences between the two groups were found also for gestational and neonatal outcomes. This work shows that different post-thaw embryo culture timings do not negatively impact pregnancy outcomes. Overall, these results are important in the context of the embryological laboratory in order to better organize the workflow and avoid unnecessary timing-related workload.
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spelling pubmed-97811742022-12-24 Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles Ciaffaglione, Marta Reschini, Marco Balli, Martina Guarneri, Cristina Palermo, Maria Carla Pinna, Monica Pisaturo, Valerio Somigliana, Edgardo Paffoni, Alessio Vigano’, Paola J Clin Med Article The frozen embryo transfer (FET) technique has been progressively used more worldwide due to improved culture conditions, as well as enhanced survival rates after vitrification. However, little is known about the effect of the post-thaw blastocyst culture duration prior to transfer on live birth rate in FET cycles. In this retrospective observational study, we evaluated the influence of two distinct post-thaw blastocyst culture spans (2–4 h versus 20–22 h) on clinical pregnancy and live birth rate. A total of n = 1927 frozen–warmed cycles were included in the analysis. Among those, n = 885 warmed blastocysts were cultured for 2–4 h, and n = 1029 were kept in culture for 20–22 h prior to transfer; the remaining blastocysts did not survive the warming protocol. We observed no significant differences in live birth and clinical pregnancy rates between the two groups. The blastocyst morphological evaluation at transfer improved following the longer culture time. No differences between the two groups were found also for gestational and neonatal outcomes. This work shows that different post-thaw embryo culture timings do not negatively impact pregnancy outcomes. Overall, these results are important in the context of the embryological laboratory in order to better organize the workflow and avoid unnecessary timing-related workload. MDPI 2022-12-15 /pmc/articles/PMC9781174/ /pubmed/36556058 http://dx.doi.org/10.3390/jcm11247444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ciaffaglione, Marta
Reschini, Marco
Balli, Martina
Guarneri, Cristina
Palermo, Maria Carla
Pinna, Monica
Pisaturo, Valerio
Somigliana, Edgardo
Paffoni, Alessio
Vigano’, Paola
Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
title Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
title_full Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
title_fullStr Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
title_full_unstemmed Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
title_short Post-Thaw Day 5 Blastocyst Culture Time Prior to Transfer Does Not Affect Assisted Reproduction Technology (ART) Outcomes in Frozen-Thawed Embryo Transfer Cycles
title_sort post-thaw day 5 blastocyst culture time prior to transfer does not affect assisted reproduction technology (art) outcomes in frozen-thawed embryo transfer cycles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781174/
https://www.ncbi.nlm.nih.gov/pubmed/36556058
http://dx.doi.org/10.3390/jcm11247444
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