Cargando…
Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster
The novel coronavirus (SARS-CoV-2) epidemic continues to be a global public crisis affecting human health. Many research groups are developing different types of vaccines to suppress the spread of SARS-CoV-2, and some vaccines have entered phase III clinical trials and have been rapidly implemented....
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781188/ https://www.ncbi.nlm.nih.gov/pubmed/36560406 http://dx.doi.org/10.3390/vaccines10121996 |
_version_ | 1784857012684718080 |
---|---|
author | Niu, Zhenye Li, Xueqi Gao, Yang Wang, Lichun Fan, Shengtao Xu, Xingli Jiang, Guorun Cui, Pingfang Li, Dandan Liao, Yun Yu, Li Zhao, Heng Zhang, Ying Li, Qihan |
author_facet | Niu, Zhenye Li, Xueqi Gao, Yang Wang, Lichun Fan, Shengtao Xu, Xingli Jiang, Guorun Cui, Pingfang Li, Dandan Liao, Yun Yu, Li Zhao, Heng Zhang, Ying Li, Qihan |
author_sort | Niu, Zhenye |
collection | PubMed |
description | The novel coronavirus (SARS-CoV-2) epidemic continues to be a global public crisis affecting human health. Many research groups are developing different types of vaccines to suppress the spread of SARS-CoV-2, and some vaccines have entered phase III clinical trials and have been rapidly implemented. Whether multiple antigen matches are necessary to induce a better immune response remains unclear. To address this question, this study tested the immunogenicity and protective effects of a SARS-CoV-2 recombinant S and N peptide vaccine in the Syrian golden hamster model. This experiment was based on two immunization methods: intradermal and intramuscular administration. Immunized hamsters were challenged with live SARS-CoV-2 14 days after booster immunization. Clinical symptoms were observed daily, and the antibody titer and viral load in each tissue were detected. The results showed that immunization of golden hamsters with the SARS-CoV-2 structural protein S alone or in combination with the N protein through different routes induced antibody responses, whereas immunization with the N protein alone did not. However, although the immunized hamsters exhibited partial alleviation of clinical symptoms when challenged with the virus, neither vaccine effectively inhibited the proliferation and replication of the challenging virus. In addition, the pathological damage in the immunized hamsters was similar to that in the control hamsters. Interestingly, the neutralizing antibody levels of all groups including immunized and nonimmunized animals increased significantly after viral challenge. In conclusion, the immune response induced by the experimental S and N polypeptide vaccines had no significant ability to prevent viral infection and pathogenicity in golden hamsters. |
format | Online Article Text |
id | pubmed-9781188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97811882022-12-24 Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster Niu, Zhenye Li, Xueqi Gao, Yang Wang, Lichun Fan, Shengtao Xu, Xingli Jiang, Guorun Cui, Pingfang Li, Dandan Liao, Yun Yu, Li Zhao, Heng Zhang, Ying Li, Qihan Vaccines (Basel) Article The novel coronavirus (SARS-CoV-2) epidemic continues to be a global public crisis affecting human health. Many research groups are developing different types of vaccines to suppress the spread of SARS-CoV-2, and some vaccines have entered phase III clinical trials and have been rapidly implemented. Whether multiple antigen matches are necessary to induce a better immune response remains unclear. To address this question, this study tested the immunogenicity and protective effects of a SARS-CoV-2 recombinant S and N peptide vaccine in the Syrian golden hamster model. This experiment was based on two immunization methods: intradermal and intramuscular administration. Immunized hamsters were challenged with live SARS-CoV-2 14 days after booster immunization. Clinical symptoms were observed daily, and the antibody titer and viral load in each tissue were detected. The results showed that immunization of golden hamsters with the SARS-CoV-2 structural protein S alone or in combination with the N protein through different routes induced antibody responses, whereas immunization with the N protein alone did not. However, although the immunized hamsters exhibited partial alleviation of clinical symptoms when challenged with the virus, neither vaccine effectively inhibited the proliferation and replication of the challenging virus. In addition, the pathological damage in the immunized hamsters was similar to that in the control hamsters. Interestingly, the neutralizing antibody levels of all groups including immunized and nonimmunized animals increased significantly after viral challenge. In conclusion, the immune response induced by the experimental S and N polypeptide vaccines had no significant ability to prevent viral infection and pathogenicity in golden hamsters. MDPI 2022-11-24 /pmc/articles/PMC9781188/ /pubmed/36560406 http://dx.doi.org/10.3390/vaccines10121996 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Niu, Zhenye Li, Xueqi Gao, Yang Wang, Lichun Fan, Shengtao Xu, Xingli Jiang, Guorun Cui, Pingfang Li, Dandan Liao, Yun Yu, Li Zhao, Heng Zhang, Ying Li, Qihan Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster |
title | Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster |
title_full | Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster |
title_fullStr | Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster |
title_full_unstemmed | Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster |
title_short | Evaluation of Immunogenicity and Clinical Protection of SARS-CoV-2 S1 and N Antigens in Syrian Golden Hamster |
title_sort | evaluation of immunogenicity and clinical protection of sars-cov-2 s1 and n antigens in syrian golden hamster |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781188/ https://www.ncbi.nlm.nih.gov/pubmed/36560406 http://dx.doi.org/10.3390/vaccines10121996 |
work_keys_str_mv | AT niuzhenye evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT lixueqi evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT gaoyang evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT wanglichun evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT fanshengtao evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT xuxingli evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT jiangguorun evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT cuipingfang evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT lidandan evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT liaoyun evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT yuli evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT zhaoheng evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT zhangying evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster AT liqihan evaluationofimmunogenicityandclinicalprotectionofsarscov2s1andnantigensinsyriangoldenhamster |