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The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland
The aim of this study was to evaluate the usefulness of the Accelerate Pheno™ system (APS) (Accelerate Diagnostics, Denver, CO, USA) for rapid laboratory diagnosis of bloodstream infections. The study included 45 positive blood samples obtained from patients hospitalized in University Hospital No. 1...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781321/ https://www.ncbi.nlm.nih.gov/pubmed/36558749 http://dx.doi.org/10.3390/pathogens11121415 |
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author | Zalas-Więcek, Patrycja Bogiel, Tomasz Gospodarek-Komkowska, Eugenia |
author_facet | Zalas-Więcek, Patrycja Bogiel, Tomasz Gospodarek-Komkowska, Eugenia |
author_sort | Zalas-Więcek, Patrycja |
collection | PubMed |
description | The aim of this study was to evaluate the usefulness of the Accelerate Pheno™ system (APS) (Accelerate Diagnostics, Denver, CO, USA) for rapid laboratory diagnosis of bloodstream infections. The study included 45 positive blood samples obtained from patients hospitalized in University Hospital No. 1 in Bydgoszcz, Poland. In 40 (88.9%) blood samples, the APS was capable of identification of at least one microorganism at the genus or species level and in 38 (84.4%) of them additionally assessed antimicrobial susceptibility. The time of identification and the time to result of antimicrobial susceptibility ranged from 1:32 to 1:42 and 5:02 to 5:36 h, respectively. Six positive blood samples revealed a poly-microbial culture. In these cases, only one out of two or three microorganisms was detected by the APS, and the system assessed antimicrobial susceptibility only for them. For 78.6% positive blood samples, agreement on identification compared to mass spectrometry was found. For all but one sample, a 96–100% compliance of the resistance category was achieved when comparing the antimicrobial susceptibility testing results to conventional methods. Using the APS, the total time to report was reduced from 13:34 to even 63:47 h compared to the standard microbiological laboratory workflow. The APS is a very useful system, especially for the rapid assessment of antimicrobial susceptibility of bacteria directly from positive blood samples, offering the greatest potential for microbiology laboratories operating around the clock. |
format | Online Article Text |
id | pubmed-9781321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97813212022-12-24 The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland Zalas-Więcek, Patrycja Bogiel, Tomasz Gospodarek-Komkowska, Eugenia Pathogens Article The aim of this study was to evaluate the usefulness of the Accelerate Pheno™ system (APS) (Accelerate Diagnostics, Denver, CO, USA) for rapid laboratory diagnosis of bloodstream infections. The study included 45 positive blood samples obtained from patients hospitalized in University Hospital No. 1 in Bydgoszcz, Poland. In 40 (88.9%) blood samples, the APS was capable of identification of at least one microorganism at the genus or species level and in 38 (84.4%) of them additionally assessed antimicrobial susceptibility. The time of identification and the time to result of antimicrobial susceptibility ranged from 1:32 to 1:42 and 5:02 to 5:36 h, respectively. Six positive blood samples revealed a poly-microbial culture. In these cases, only one out of two or three microorganisms was detected by the APS, and the system assessed antimicrobial susceptibility only for them. For 78.6% positive blood samples, agreement on identification compared to mass spectrometry was found. For all but one sample, a 96–100% compliance of the resistance category was achieved when comparing the antimicrobial susceptibility testing results to conventional methods. Using the APS, the total time to report was reduced from 13:34 to even 63:47 h compared to the standard microbiological laboratory workflow. The APS is a very useful system, especially for the rapid assessment of antimicrobial susceptibility of bacteria directly from positive blood samples, offering the greatest potential for microbiology laboratories operating around the clock. MDPI 2022-11-24 /pmc/articles/PMC9781321/ /pubmed/36558749 http://dx.doi.org/10.3390/pathogens11121415 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zalas-Więcek, Patrycja Bogiel, Tomasz Gospodarek-Komkowska, Eugenia The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland |
title | The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland |
title_full | The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland |
title_fullStr | The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland |
title_full_unstemmed | The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland |
title_short | The Accelerate Pheno™ System—A New Tool in Microbiological Diagnostics of Bloodstream Infections: A Pilot Study from Poland |
title_sort | accelerate pheno™ system—a new tool in microbiological diagnostics of bloodstream infections: a pilot study from poland |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781321/ https://www.ncbi.nlm.nih.gov/pubmed/36558749 http://dx.doi.org/10.3390/pathogens11121415 |
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