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Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor
Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a sc...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781371/ https://www.ncbi.nlm.nih.gov/pubmed/36559794 http://dx.doi.org/10.3390/polym14245427 |
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author | Rojas-Rojas, Laura Espinoza-Álvarez, María Laura Castro-Piedra, Silvia Ulloa-Fernández, Andrea Vargas-Segura, Walter Guillén-Girón, Teodolito |
author_facet | Rojas-Rojas, Laura Espinoza-Álvarez, María Laura Castro-Piedra, Silvia Ulloa-Fernández, Andrea Vargas-Segura, Walter Guillén-Girón, Teodolito |
author_sort | Rojas-Rojas, Laura |
collection | PubMed |
description | Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a scaffold under desired characteristics, and apply mechanical stimulation to scaffolds. Polymers are often used for fabricating tissue-engineering scaffolds. In this study, polycaprolactone (PCL) collagen-coated microfilament scaffolds were cell-seeded with C2C12 myoblasts; then, these were grown inside a custom-built bioreactor. Cell attachment and proliferation on the scaffolds were investigated. A loading pattern was used for mechanical stimulation of the cell-seeded scaffolds. Results showed that the microfilaments provided a suitable scaffold for myoblast anchorage and that the custom-built bioreactor provided a qualified environment for the survival of the myoblasts on the polymeric scaffold. This PCL-based microfilament scaffold located inside the bioreactor proved to be a promising structure for the study of skeletal muscle models and can be used for mechanical stimulation studies in tissue engineering applications. |
format | Online Article Text |
id | pubmed-9781371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97813712022-12-24 Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor Rojas-Rojas, Laura Espinoza-Álvarez, María Laura Castro-Piedra, Silvia Ulloa-Fernández, Andrea Vargas-Segura, Walter Guillén-Girón, Teodolito Polymers (Basel) Article Tissue engineering aims to develop in-vitro substitutes of native tissues. One approach of tissue engineering relies on using bioreactors combined with biomimetic scaffolds to produce study models or in-vitro substitutes. Bioreactors provide control over environmental parameters, place and hold a scaffold under desired characteristics, and apply mechanical stimulation to scaffolds. Polymers are often used for fabricating tissue-engineering scaffolds. In this study, polycaprolactone (PCL) collagen-coated microfilament scaffolds were cell-seeded with C2C12 myoblasts; then, these were grown inside a custom-built bioreactor. Cell attachment and proliferation on the scaffolds were investigated. A loading pattern was used for mechanical stimulation of the cell-seeded scaffolds. Results showed that the microfilaments provided a suitable scaffold for myoblast anchorage and that the custom-built bioreactor provided a qualified environment for the survival of the myoblasts on the polymeric scaffold. This PCL-based microfilament scaffold located inside the bioreactor proved to be a promising structure for the study of skeletal muscle models and can be used for mechanical stimulation studies in tissue engineering applications. MDPI 2022-12-11 /pmc/articles/PMC9781371/ /pubmed/36559794 http://dx.doi.org/10.3390/polym14245427 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rojas-Rojas, Laura Espinoza-Álvarez, María Laura Castro-Piedra, Silvia Ulloa-Fernández, Andrea Vargas-Segura, Walter Guillén-Girón, Teodolito Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor |
title | Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor |
title_full | Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor |
title_fullStr | Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor |
title_full_unstemmed | Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor |
title_short | Muscle-like Scaffolds for Biomechanical Stimulation in a Custom-Built Bioreactor |
title_sort | muscle-like scaffolds for biomechanical stimulation in a custom-built bioreactor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781371/ https://www.ncbi.nlm.nih.gov/pubmed/36559794 http://dx.doi.org/10.3390/polym14245427 |
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