Cargando…

Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves

Drought intensity modifies the assimilatory pathway of glutathione (GSH) synthesis. Abscisic acid (ABA) is a representative signaling hormone involved in regulating plant stress responses. This study aimed to investigate an interactive regulation of sulfate and/or ABA in GSH metabolism and redox. Th...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Bok-Rye, Park, Sang-Hyun, La, Van Hien, Bae, Dong-Won, Kim, Tae-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781433/
https://www.ncbi.nlm.nih.gov/pubmed/36557228
http://dx.doi.org/10.3390/metabo12121190
_version_ 1784857073103667200
author Lee, Bok-Rye
Park, Sang-Hyun
La, Van Hien
Bae, Dong-Won
Kim, Tae-Hwan
author_facet Lee, Bok-Rye
Park, Sang-Hyun
La, Van Hien
Bae, Dong-Won
Kim, Tae-Hwan
author_sort Lee, Bok-Rye
collection PubMed
description Drought intensity modifies the assimilatory pathway of glutathione (GSH) synthesis. Abscisic acid (ABA) is a representative signaling hormone involved in regulating plant stress responses. This study aimed to investigate an interactive regulation of sulfate and/or ABA in GSH metabolism and redox. The drought-responsive alterations in sulfate assimilation and GSH-based redox reactions were assessed relative to ABA responses on the time-course of drought intensity. Drought-responsive H(2)O(2) concentrations were divided into two distinct phases—an initial 4 days of no change (Ψ(w) ≥ −0.49 MPa) and a phase of higher accumulation during the late phase of the drought (days 10–14; Ψ(w) ≤ −1.34 MPa). During the early phase of the drought, GSH/GSSG redox state turned to the slightly reduced state with a transient increase in GSH, resulting from a strong activation of H(2)O(2) scavenging enzymes, ascorbate peroxidase (APOX) and glutathione reductase (GR). The late phase of the drought was characterized by a decrease in GSH due to cysteine accumulation, shifting GSH- and NADPH-based redox states to higher oxidization, increasing sulfate and ABA in xylem, and causing ABA accumulation in leaves. Regression analysis revealed that sulfate in xylem sap was positively correlated with H(2)O(2) concentrations and ABA was closely related to decreases in the GSH pool and the oxidation of GSH catalyzed by glutathione peroxidase (GPOX). These results indicate that drought-induced oxidation proceeds through the suppression of GSH synthesis and further GSH oxidation in a sulfate-activated ABA-dependent manner.
format Online
Article
Text
id pubmed-9781433
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97814332022-12-24 Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves Lee, Bok-Rye Park, Sang-Hyun La, Van Hien Bae, Dong-Won Kim, Tae-Hwan Metabolites Article Drought intensity modifies the assimilatory pathway of glutathione (GSH) synthesis. Abscisic acid (ABA) is a representative signaling hormone involved in regulating plant stress responses. This study aimed to investigate an interactive regulation of sulfate and/or ABA in GSH metabolism and redox. The drought-responsive alterations in sulfate assimilation and GSH-based redox reactions were assessed relative to ABA responses on the time-course of drought intensity. Drought-responsive H(2)O(2) concentrations were divided into two distinct phases—an initial 4 days of no change (Ψ(w) ≥ −0.49 MPa) and a phase of higher accumulation during the late phase of the drought (days 10–14; Ψ(w) ≤ −1.34 MPa). During the early phase of the drought, GSH/GSSG redox state turned to the slightly reduced state with a transient increase in GSH, resulting from a strong activation of H(2)O(2) scavenging enzymes, ascorbate peroxidase (APOX) and glutathione reductase (GR). The late phase of the drought was characterized by a decrease in GSH due to cysteine accumulation, shifting GSH- and NADPH-based redox states to higher oxidization, increasing sulfate and ABA in xylem, and causing ABA accumulation in leaves. Regression analysis revealed that sulfate in xylem sap was positively correlated with H(2)O(2) concentrations and ABA was closely related to decreases in the GSH pool and the oxidation of GSH catalyzed by glutathione peroxidase (GPOX). These results indicate that drought-induced oxidation proceeds through the suppression of GSH synthesis and further GSH oxidation in a sulfate-activated ABA-dependent manner. MDPI 2022-11-29 /pmc/articles/PMC9781433/ /pubmed/36557228 http://dx.doi.org/10.3390/metabo12121190 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Bok-Rye
Park, Sang-Hyun
La, Van Hien
Bae, Dong-Won
Kim, Tae-Hwan
Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves
title Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves
title_full Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves
title_fullStr Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves
title_full_unstemmed Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves
title_short Drought-Induced Xylem Sulfate Activates the ABA-Mediated Regulation of Sulfate Assimilation and Glutathione Redox in Brassica napus Leaves
title_sort drought-induced xylem sulfate activates the aba-mediated regulation of sulfate assimilation and glutathione redox in brassica napus leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781433/
https://www.ncbi.nlm.nih.gov/pubmed/36557228
http://dx.doi.org/10.3390/metabo12121190
work_keys_str_mv AT leebokrye droughtinducedxylemsulfateactivatestheabamediatedregulationofsulfateassimilationandglutathioneredoxinbrassicanapusleaves
AT parksanghyun droughtinducedxylemsulfateactivatestheabamediatedregulationofsulfateassimilationandglutathioneredoxinbrassicanapusleaves
AT lavanhien droughtinducedxylemsulfateactivatestheabamediatedregulationofsulfateassimilationandglutathioneredoxinbrassicanapusleaves
AT baedongwon droughtinducedxylemsulfateactivatestheabamediatedregulationofsulfateassimilationandglutathioneredoxinbrassicanapusleaves
AT kimtaehwan droughtinducedxylemsulfateactivatestheabamediatedregulationofsulfateassimilationandglutathioneredoxinbrassicanapusleaves