Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina

Two compounds 1 and 2 were isolated from the culture broth of Lepista luscina. This is the first time that compound 1 was isolated from a natural source. The structure of compound 1 was identified via 1D and 2D NMR and HRESIMS data. Compounds 1 and 2 along with 8-nitrotryptanthrin (4) were evaluated...

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Autores principales: Kotajima, Mihaya, Choi, Jae-Hoon, Kondo, Mitsuru, D’Alessandro-Gabazza, Corina N., Toda, Masaaki, Yasuma, Taro, Gabazza, Esteban C., Miwa, Yukihiro, Shoda, Chiho, Lee, Deokho, Nakai, Ayaka, Kurihara, Toshihide, Wu, Jing, Hirai, Hirofumi, Kawagishi, Hirokazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781456/
https://www.ncbi.nlm.nih.gov/pubmed/36558053
http://dx.doi.org/10.3390/molecules27248925
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author Kotajima, Mihaya
Choi, Jae-Hoon
Kondo, Mitsuru
D’Alessandro-Gabazza, Corina N.
Toda, Masaaki
Yasuma, Taro
Gabazza, Esteban C.
Miwa, Yukihiro
Shoda, Chiho
Lee, Deokho
Nakai, Ayaka
Kurihara, Toshihide
Wu, Jing
Hirai, Hirofumi
Kawagishi, Hirokazu
author_facet Kotajima, Mihaya
Choi, Jae-Hoon
Kondo, Mitsuru
D’Alessandro-Gabazza, Corina N.
Toda, Masaaki
Yasuma, Taro
Gabazza, Esteban C.
Miwa, Yukihiro
Shoda, Chiho
Lee, Deokho
Nakai, Ayaka
Kurihara, Toshihide
Wu, Jing
Hirai, Hirofumi
Kawagishi, Hirokazu
author_sort Kotajima, Mihaya
collection PubMed
description Two compounds 1 and 2 were isolated from the culture broth of Lepista luscina. This is the first time that compound 1 was isolated from a natural source. The structure of compound 1 was identified via 1D and 2D NMR and HRESIMS data. Compounds 1 and 2 along with 8-nitrotryptanthrin (4) were evaluated for their biological activities using the A549 lung cancer cell line. As a result, 1 and 2 inhibited the expression of Axl and immune checkpoint molecules. In addition, compounds 1, 2 and 4 were tested for HIF inhibitory activity. Compound 2 demonstrated statistically significant HIF inhibitory effects on NIH3T3 cells and 1 and 2 against ARPE19 cells.
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spelling pubmed-97814562022-12-24 Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina Kotajima, Mihaya Choi, Jae-Hoon Kondo, Mitsuru D’Alessandro-Gabazza, Corina N. Toda, Masaaki Yasuma, Taro Gabazza, Esteban C. Miwa, Yukihiro Shoda, Chiho Lee, Deokho Nakai, Ayaka Kurihara, Toshihide Wu, Jing Hirai, Hirofumi Kawagishi, Hirokazu Molecules Article Two compounds 1 and 2 were isolated from the culture broth of Lepista luscina. This is the first time that compound 1 was isolated from a natural source. The structure of compound 1 was identified via 1D and 2D NMR and HRESIMS data. Compounds 1 and 2 along with 8-nitrotryptanthrin (4) were evaluated for their biological activities using the A549 lung cancer cell line. As a result, 1 and 2 inhibited the expression of Axl and immune checkpoint molecules. In addition, compounds 1, 2 and 4 were tested for HIF inhibitory activity. Compound 2 demonstrated statistically significant HIF inhibitory effects on NIH3T3 cells and 1 and 2 against ARPE19 cells. MDPI 2022-12-15 /pmc/articles/PMC9781456/ /pubmed/36558053 http://dx.doi.org/10.3390/molecules27248925 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kotajima, Mihaya
Choi, Jae-Hoon
Kondo, Mitsuru
D’Alessandro-Gabazza, Corina N.
Toda, Masaaki
Yasuma, Taro
Gabazza, Esteban C.
Miwa, Yukihiro
Shoda, Chiho
Lee, Deokho
Nakai, Ayaka
Kurihara, Toshihide
Wu, Jing
Hirai, Hirofumi
Kawagishi, Hirokazu
Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina
title Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina
title_full Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina
title_fullStr Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina
title_full_unstemmed Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina
title_short Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina
title_sort axl, immune checkpoint molecules and hif inhibitors from the culture broth of lepista luscina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781456/
https://www.ncbi.nlm.nih.gov/pubmed/36558053
http://dx.doi.org/10.3390/molecules27248925
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