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Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and prov...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781480/ https://www.ncbi.nlm.nih.gov/pubmed/36555532 http://dx.doi.org/10.3390/ijms232415895 |
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author | Ball, David A. Jalloh, Binta Karpova, Tatiana S. |
author_facet | Ball, David A. Jalloh, Binta Karpova, Tatiana S. |
author_sort | Ball, David A. |
collection | PubMed |
description | Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes. |
format | Online Article Text |
id | pubmed-9781480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97814802022-12-24 Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics Ball, David A. Jalloh, Binta Karpova, Tatiana S. Int J Mol Sci Review Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes. MDPI 2022-12-14 /pmc/articles/PMC9781480/ /pubmed/36555532 http://dx.doi.org/10.3390/ijms232415895 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Ball, David A. Jalloh, Binta Karpova, Tatiana S. Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics |
title | Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics |
title_full | Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics |
title_fullStr | Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics |
title_full_unstemmed | Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics |
title_short | Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics |
title_sort | impact of saccharomyces cerevisiae on the field of single-molecule biophysics |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781480/ https://www.ncbi.nlm.nih.gov/pubmed/36555532 http://dx.doi.org/10.3390/ijms232415895 |
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