Cargando…

Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics

Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and prov...

Descripción completa

Detalles Bibliográficos
Autores principales: Ball, David A., Jalloh, Binta, Karpova, Tatiana S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781480/
https://www.ncbi.nlm.nih.gov/pubmed/36555532
http://dx.doi.org/10.3390/ijms232415895
_version_ 1784857084503785472
author Ball, David A.
Jalloh, Binta
Karpova, Tatiana S.
author_facet Ball, David A.
Jalloh, Binta
Karpova, Tatiana S.
author_sort Ball, David A.
collection PubMed
description Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes.
format Online
Article
Text
id pubmed-9781480
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-97814802022-12-24 Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics Ball, David A. Jalloh, Binta Karpova, Tatiana S. Int J Mol Sci Review Cellular functions depend on the dynamic assembly of protein regulator complexes at specific cellular locations. Single Molecule Tracking (SMT) is a method of choice for the biochemical characterization of protein dynamics in vitro and in vivo. SMT follows individual molecules in live cells and provides direct information about their behavior. SMT was successfully applied to mammalian models. However, mammalian cells provide a complex environment where protein mobility depends on numerous factors that are difficult to control experimentally. Therefore, yeast cells, which are unicellular and well-studied with a small and completely sequenced genome, provide an attractive alternative for SMT. The simplicity of organization, ease of genetic manipulation, and tolerance to gene fusions all make yeast a great model for quantifying the kinetics of major enzymes, membrane proteins, and nuclear and cellular bodies. However, very few researchers apply SMT techniques to yeast. Our goal is to promote SMT in yeast to a wider research community. Our review serves a dual purpose. We explain how SMT is conducted in yeast cells, and we discuss the latest insights from yeast SMT while putting them in perspective with SMT of higher eukaryotes. MDPI 2022-12-14 /pmc/articles/PMC9781480/ /pubmed/36555532 http://dx.doi.org/10.3390/ijms232415895 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Ball, David A.
Jalloh, Binta
Karpova, Tatiana S.
Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
title Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
title_full Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
title_fullStr Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
title_full_unstemmed Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
title_short Impact of Saccharomyces cerevisiae on the Field of Single-Molecule Biophysics
title_sort impact of saccharomyces cerevisiae on the field of single-molecule biophysics
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781480/
https://www.ncbi.nlm.nih.gov/pubmed/36555532
http://dx.doi.org/10.3390/ijms232415895
work_keys_str_mv AT balldavida impactofsaccharomycescerevisiaeonthefieldofsinglemoleculebiophysics
AT jallohbinta impactofsaccharomycescerevisiaeonthefieldofsinglemoleculebiophysics
AT karpovatatianas impactofsaccharomycescerevisiaeonthefieldofsinglemoleculebiophysics