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Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)

Pomegranate (Punica granatum, L.) is a fruit tree that is increasingly popular worldwide due to the health-related properties of the fruit juice. While several studies highlighted the rich phytochemical diversity, few efforts have been devoted to an integrative understanding of the level of diversit...

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Autores principales: Parashuram, Shilpa, Singh, Nripendra Vikram, Gaikwad, Nilesh Nivrutti, Corrado, Giandomenico, Roopa Sowjanya, P., Basile, Boris, Devaraja, Nitesh Shirur, Chandra, Ram, Babu, Karuppannan Dhinesh, Patil, Prakash Goudappa, Kumar, Pradeep, Singh, Akath, Marathe, Rajiv Arvind
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781629/
https://www.ncbi.nlm.nih.gov/pubmed/36559629
http://dx.doi.org/10.3390/plants11243518
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author Parashuram, Shilpa
Singh, Nripendra Vikram
Gaikwad, Nilesh Nivrutti
Corrado, Giandomenico
Roopa Sowjanya, P.
Basile, Boris
Devaraja, Nitesh Shirur
Chandra, Ram
Babu, Karuppannan Dhinesh
Patil, Prakash Goudappa
Kumar, Pradeep
Singh, Akath
Marathe, Rajiv Arvind
author_facet Parashuram, Shilpa
Singh, Nripendra Vikram
Gaikwad, Nilesh Nivrutti
Corrado, Giandomenico
Roopa Sowjanya, P.
Basile, Boris
Devaraja, Nitesh Shirur
Chandra, Ram
Babu, Karuppannan Dhinesh
Patil, Prakash Goudappa
Kumar, Pradeep
Singh, Akath
Marathe, Rajiv Arvind
author_sort Parashuram, Shilpa
collection PubMed
description Pomegranate (Punica granatum, L.) is a fruit tree that is increasingly popular worldwide due to the health-related properties of the fruit juice. While several studies highlighted the rich phytochemical diversity, few efforts have been devoted to an integrative understanding of the level of diversity of this species. This study investigated the diversity of 40 pomegranate accessions in an Indian ex situ collection by using twenty-nine morphological traits, six biochemical parameters, and twenty-nine Simple Sequence Repeats (SSR) markers. Among the evaluated traits, fruit volume (23.34% CV), fruit weight (21.12% CV), and fruit color (*a) (22.69 % CV) largely contributed to the morphological classification. Based on Mahalanobis D(2) distance and Tocher’s clustering, the 40 pomegranate accessions were grouped into eight clusters, partly consistent with their origin. Specifically, cultivars introduced from foreign countries were present in distinct clusters. The SSR marker analysis generated 66 alleles. The observed heterozygosity values ranged from 0.05 to 0.63, with a mean value of 0.30. Maximum molecular genetic dissimilarity was observed between ‘IC-318720′ and ‘Gul-e-Shah Red’ (0.30). The neighbor-joining dendrogram separated wild accessions from cultivated varieties. The combination of morphological, biochemical, and molecular characterization allowed for comprehensively characterizing the pomegranate diversity and provided information on the relationships between the different aspects of the diversity. This work also suggests that the origin of the accessions is an important factor of discrimination and that the level of admixture between local and foreign material is currently limited.
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spelling pubmed-97816292022-12-24 Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.) Parashuram, Shilpa Singh, Nripendra Vikram Gaikwad, Nilesh Nivrutti Corrado, Giandomenico Roopa Sowjanya, P. Basile, Boris Devaraja, Nitesh Shirur Chandra, Ram Babu, Karuppannan Dhinesh Patil, Prakash Goudappa Kumar, Pradeep Singh, Akath Marathe, Rajiv Arvind Plants (Basel) Article Pomegranate (Punica granatum, L.) is a fruit tree that is increasingly popular worldwide due to the health-related properties of the fruit juice. While several studies highlighted the rich phytochemical diversity, few efforts have been devoted to an integrative understanding of the level of diversity of this species. This study investigated the diversity of 40 pomegranate accessions in an Indian ex situ collection by using twenty-nine morphological traits, six biochemical parameters, and twenty-nine Simple Sequence Repeats (SSR) markers. Among the evaluated traits, fruit volume (23.34% CV), fruit weight (21.12% CV), and fruit color (*a) (22.69 % CV) largely contributed to the morphological classification. Based on Mahalanobis D(2) distance and Tocher’s clustering, the 40 pomegranate accessions were grouped into eight clusters, partly consistent with their origin. Specifically, cultivars introduced from foreign countries were present in distinct clusters. The SSR marker analysis generated 66 alleles. The observed heterozygosity values ranged from 0.05 to 0.63, with a mean value of 0.30. Maximum molecular genetic dissimilarity was observed between ‘IC-318720′ and ‘Gul-e-Shah Red’ (0.30). The neighbor-joining dendrogram separated wild accessions from cultivated varieties. The combination of morphological, biochemical, and molecular characterization allowed for comprehensively characterizing the pomegranate diversity and provided information on the relationships between the different aspects of the diversity. This work also suggests that the origin of the accessions is an important factor of discrimination and that the level of admixture between local and foreign material is currently limited. MDPI 2022-12-14 /pmc/articles/PMC9781629/ /pubmed/36559629 http://dx.doi.org/10.3390/plants11243518 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parashuram, Shilpa
Singh, Nripendra Vikram
Gaikwad, Nilesh Nivrutti
Corrado, Giandomenico
Roopa Sowjanya, P.
Basile, Boris
Devaraja, Nitesh Shirur
Chandra, Ram
Babu, Karuppannan Dhinesh
Patil, Prakash Goudappa
Kumar, Pradeep
Singh, Akath
Marathe, Rajiv Arvind
Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)
title Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)
title_full Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)
title_fullStr Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)
title_full_unstemmed Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)
title_short Morphological, Biochemical, and Molecular Diversity of an Indian Ex Situ Collection of Pomegranate (Punica granatum L.)
title_sort morphological, biochemical, and molecular diversity of an indian ex situ collection of pomegranate (punica granatum l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781629/
https://www.ncbi.nlm.nih.gov/pubmed/36559629
http://dx.doi.org/10.3390/plants11243518
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