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Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells
Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. S...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781670/ https://www.ncbi.nlm.nih.gov/pubmed/36560654 http://dx.doi.org/10.3390/v14122651 |
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author | Chen, Jian-Hua Vanslembrouck, Bieke Ekman, Axel Aho, Vesa Larabell, Carolyn A. Le Gros, Mark A. Vihinen-Ranta, Maija Weinhardt, Venera |
author_facet | Chen, Jian-Hua Vanslembrouck, Bieke Ekman, Axel Aho, Vesa Larabell, Carolyn A. Le Gros, Mark A. Vihinen-Ranta, Maija Weinhardt, Venera |
author_sort | Chen, Jian-Hua |
collection | PubMed |
description | Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporospatial remodeling of cells during the infection and observed changes in cellular structures, such as the presence of cytoplasmic stress granules and multivesicular structures, formation of nuclear virus-induced dense bodies, and aggregates of capsids. Our results demonstrate the power of SXT imaging for scouting virus-induced changes in infected cells and understanding the orchestration of virus-host remodeling quantitatively. |
format | Online Article Text |
id | pubmed-9781670 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97816702022-12-24 Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells Chen, Jian-Hua Vanslembrouck, Bieke Ekman, Axel Aho, Vesa Larabell, Carolyn A. Le Gros, Mark A. Vihinen-Ranta, Maija Weinhardt, Venera Viruses Article Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporospatial remodeling of cells during the infection and observed changes in cellular structures, such as the presence of cytoplasmic stress granules and multivesicular structures, formation of nuclear virus-induced dense bodies, and aggregates of capsids. Our results demonstrate the power of SXT imaging for scouting virus-induced changes in infected cells and understanding the orchestration of virus-host remodeling quantitatively. MDPI 2022-11-27 /pmc/articles/PMC9781670/ /pubmed/36560654 http://dx.doi.org/10.3390/v14122651 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Jian-Hua Vanslembrouck, Bieke Ekman, Axel Aho, Vesa Larabell, Carolyn A. Le Gros, Mark A. Vihinen-Ranta, Maija Weinhardt, Venera Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells |
title | Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells |
title_full | Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells |
title_fullStr | Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells |
title_full_unstemmed | Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells |
title_short | Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells |
title_sort | soft x-ray tomography reveals hsv-1-induced remodeling of human b cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781670/ https://www.ncbi.nlm.nih.gov/pubmed/36560654 http://dx.doi.org/10.3390/v14122651 |
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