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Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages

In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens...

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Autores principales: Han, Dong-Geun, Bae, Min-Jun, An, Bong-Jeon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781689/
https://www.ncbi.nlm.nih.gov/pubmed/36557931
http://dx.doi.org/10.3390/molecules27248797
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author Han, Dong-Geun
Bae, Min-Jun
An, Bong-Jeon
author_facet Han, Dong-Geun
Bae, Min-Jun
An, Bong-Jeon
author_sort Han, Dong-Geun
collection PubMed
description In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in M. pruriens through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that M. pruriens extracts have the potential for use as an anti-inflammatory material.
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spelling pubmed-97816892022-12-24 Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages Han, Dong-Geun Bae, Min-Jun An, Bong-Jeon Molecules Article In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in M. pruriens through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that M. pruriens extracts have the potential for use as an anti-inflammatory material. MDPI 2022-12-12 /pmc/articles/PMC9781689/ /pubmed/36557931 http://dx.doi.org/10.3390/molecules27248797 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Dong-Geun
Bae, Min-Jun
An, Bong-Jeon
Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
title Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
title_full Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
title_fullStr Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
title_full_unstemmed Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
title_short Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
title_sort anti-inflammatory activity of velvet bean (mucuna pruriens) substances in lps−stimulated raw 264.7 macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781689/
https://www.ncbi.nlm.nih.gov/pubmed/36557931
http://dx.doi.org/10.3390/molecules27248797
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