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Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages
In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781689/ https://www.ncbi.nlm.nih.gov/pubmed/36557931 http://dx.doi.org/10.3390/molecules27248797 |
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author | Han, Dong-Geun Bae, Min-Jun An, Bong-Jeon |
author_facet | Han, Dong-Geun Bae, Min-Jun An, Bong-Jeon |
author_sort | Han, Dong-Geun |
collection | PubMed |
description | In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in M. pruriens through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that M. pruriens extracts have the potential for use as an anti-inflammatory material. |
format | Online Article Text |
id | pubmed-9781689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97816892022-12-24 Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages Han, Dong-Geun Bae, Min-Jun An, Bong-Jeon Molecules Article In this study, Mucuna pruriens extracts were used to verify their application as a natural-based raw material with anti-inflammatory function. A nitric oxide inhibition activity assay showed that M. pruriens extracted with hot water (MW), M. pruriens extracted with 70% ethanol (ME), and M. pruriens extracted with 70% acetone (MA) presented NO inhibition activity; among them, MW and ME demonstrated the best activity and were selected for Western blot analysis. After identifying the expression patterns of inflammation-related proteins, such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), through Western blots, both MW and ME showed inhibition patterns. As a result of analyzing L-DOPA contained in M. pruriens through ultra-performance liquid chromatography (UPLC), high L-DOPA content was detected in MW, ME, and MA. Therefore, it can be concluded that M. pruriens extracts have the potential for use as an anti-inflammatory material. MDPI 2022-12-12 /pmc/articles/PMC9781689/ /pubmed/36557931 http://dx.doi.org/10.3390/molecules27248797 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Han, Dong-Geun Bae, Min-Jun An, Bong-Jeon Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages |
title | Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages |
title_full | Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages |
title_fullStr | Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages |
title_full_unstemmed | Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages |
title_short | Anti-Inflammatory Activity of Velvet Bean (Mucuna pruriens) Substances in LPS−Stimulated RAW 264.7 Macrophages |
title_sort | anti-inflammatory activity of velvet bean (mucuna pruriens) substances in lps−stimulated raw 264.7 macrophages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9781689/ https://www.ncbi.nlm.nih.gov/pubmed/36557931 http://dx.doi.org/10.3390/molecules27248797 |
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